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    CHEMILUMINESCENT COMPOSITIONS, METHODS, ASSAYS AND KITS FOR OXIDATIVE ENZYMES
    2.
    发明申请
    CHEMILUMINESCENT COMPOSITIONS, METHODS, ASSAYS AND KITS FOR OXIDATIVE ENZYMES 有权
    氧化酶的显色组合物，方法，测定和试剂

    公开(公告)号：US20150284766A1

    公开(公告)日：2015-10-08

    申请号：US14694758

    申请日：2015-04-23

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Rouh-Rong Juo ,  Brooks Edwards ,  Melissa GEE ,  Zhixian WANG ,  Kathleen SKAARE

    IPC: C12Q1/26 ,  C07D409/04 ,  C07D323/00

    CPC classification number: C12Q1/26 ,  C07D305/14 ,  C07D321/00 ,  C07D323/00 ,  C07D407/04 ,  C07D409/04 ,  C07D493/04 ,  C07D493/10 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2333/90258

    Abstract: Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein R1 is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I.Kits, methods and assays are also disclosed that comprise the dioxetane compounds.

    Abstract translation: 描述了用于氧化酶的化学发光组合物，方法，测定和试剂盒。 进一步公开的是以下形式的二氧杂环丁烷化合物：其中R可以独立地为任何为二氧杂环丁烷提供稳定性的支链烷基或环烷基，或者其中两个R基团一起形成与二氧杂环丁烷环结合的环烷基或多环烷基部分，其中每个R基团或 螺结合部分可以是未取代的或被一个或多个吸电子基团或给电子基团或提供优先氧化同功酶底物识别的基团取代，并且其中R1是芳基或1-20个碳原子的烷基， 其可以任选地被1个或多个卤素原子取代，并且其中T是在二氧杂环丁烷I的酶活化分解时能够发光的芳基或杂芳基环。还公开了包含二恶丁环化合物的方法和测定法。

    Solid Phases Optimized for Chemiluminescent Detection
    4.
    发明申请
    Solid Phases Optimized for Chemiluminescent Detection 审中-公开
    固化相用于化学发光检测

    公开(公告)号：US20140186831A1

    公开(公告)日：2014-07-03

    申请号：US14070138

    申请日：2013-11-01

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brooks Edwards ,  Timothy Geiser ,  Steven Menchen ,  Alison Sparks ,  John Voyta

    IPC: C12Q1/68

    CPC classification number: C12Q1/6823 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  G01N33/54393 ,  Y10S436/805 ,  Y10S436/823

    Abstract: Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a cationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.

    Abstract translation: 提供了化学发光测定的固体支持物。 固体支持物包括共价或物理连接到支持物表面上的多个探针和结合到表面或载体的大部分中的化学发光增强部分。 固体支持物可以是包含邻近阳离子微凝胶层的上探针结合层（例如，吖内酯聚合物层或多孔功能性聚酰胺层）的多层载体。 吖内酯官能聚合物可以是与乙二胺交联的二甲基丙烯酰胺和乙烯基内酯的共聚物。 阳离子微凝胶层可以是交联的含季铵盐的聚合物。 还提供了使用固体支持物进行化学发光测定的方法和试剂盒。 试剂盒包括二氧杂环丁烷底物，生物聚合物探针 - 酶复合物和固体支持物。 固体支持物可以是与阳离子微凝胶层相邻的吖内酯官能聚合物层; 与阳离子微凝胶层相邻的多孔聚酰胺官能层; 或季铵化的吖内酯官能聚合物层。

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