公开(公告)号：US10150992B2

公开(公告)日：2018-12-11

申请号：US15202487

申请日：2016-07-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Guobing Xiang ,  Prasanna Krishnan Thwar ,  Jeffrey Frediani ,  Allen Wong ,  Alfred Lui ,  Lily Lu

IPC: C12Q1/686 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.

公开(公告)号：US10724094B2

公开(公告)日：2020-07-28

申请号：US15945348

申请日：2018-04-04

Applicant: Life Technologies Corporation

Inventor： Steven M. Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer H. Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US09868826B2

公开(公告)日：2018-01-16

申请号：US15200533

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Steven M. Menchen ,  Talha M. Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08J3/075 ,  C08F220/58 ,  C08F8/12 ,  C08F220/70 ,  C08F230/08

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US10189956B2

公开(公告)日：2019-01-29

申请号：US15871863

申请日：2018-01-15

Applicant: Life Technologies AS ,  Life Technologies Corporation

Inventor： Geir Fonnum ,  Steven M. Menchen ,  M. Talha Gokmen ,  Pontus Lundberg ,  Prasanna Krishnan Thwar ,  Alfred Lui ,  Lily Lu ,  Wolfgang Hinz ,  Lene Husaboe ,  Elisabeth Breivold ,  Astrid Evenroed Molteberg ,  Synne Larsen

IPC: C08F8/12 ,  C08J3/075 ,  C08F220/58 ,  C08F220/70 ,  C08F230/08

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

公开(公告)号：US09139667B2

公开(公告)日：2015-09-22

申请号：US13763066

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C08F8/40 ,  C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

Abstract translation: 缀合基质的方法包括将与生物分子相关的抗衡离子与亲脂抗衡离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在非水溶剂存在下将生物分子复合物偶联到底物 。

公开(公告)号：US09938577B2

公开(公告)日：2018-04-10

申请号：US14855705

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer H. Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C12Q1/68 ,  C08F8/40

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US09458485B2

公开(公告)日：2016-10-04

申请号：US14799259

申请日：2015-07-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Krishnan Thwar ,  Todd Roswech ,  John Andrew Sheridan

IPC: C12P19/34 ,  C12M1/26 ,  B01F15/00 ,  B04B11/02 ,  B01F5/04 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  B04B5/04 ,  B04B7/02 ,  B01F3/08 ,  C12Q1/68

CPC classification number: B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F15/00 ,  B01F2215/0037 ,  B01L3/5021 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0409 ,  B04B5/0414 ,  B04B7/02 ,  B04B11/02 ,  B04B2007/025 ,  C12M33/10 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2523/32 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

公开(公告)号：US20160002723A1

公开(公告)日：2016-01-07

申请号：US14855705

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. MENCHEN ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer H. Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US11702696B2

公开(公告)日：2023-07-18

申请号：US16938222

申请日：2020-07-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Steven M. Menchen ,  Alan Blanchard ,  Luisa Andruzzi ,  Shaheer Khan ,  Dmitriy Gremyachinskiy ,  Alfred Lui ,  Craig Stolarczyk ,  Tanya Sokolsky ,  Prasanna Krishnan Thwar

IPC: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834

CPC classification number: C12Q1/6876 ,  C08F8/40 ,  C12Q1/6834 ,  C12Q1/6834 ,  C12Q2523/101 ,  C12Q2563/149 ,  C12Q2565/119

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

公开(公告)号：US09776146B2

公开(公告)日：2017-10-03

申请号：US14696642

申请日：2015-04-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Schultz ,  John Nobile ,  Brian Reed ,  Prasanna Krishnan Thwar ,  Todd Roswech

IPC: B01F5/06 ,  C12M1/34 ,  C12M3/00 ,  B01F3/08 ,  B01F5/04 ,  B01F13/00 ,  B04B5/04 ,  B04B7/02 ,  B01L3/00

CPC classification number: B01F5/0602 ,  B01F3/0807 ,  B01F5/0485 ,  B01F13/0059 ,  B01F2215/0037 ,  B01L3/502784 ,  B04B5/0414 ,  B04B7/02

Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.