公开(公告)号：US11175204B1

公开(公告)日：2021-11-16

申请号：US15192107

申请日：2016-06-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jixiang Liu ,  Hee Chol Kang ,  Kyle Gee ,  Christopher Langsdorf ,  Jolene Bradford ,  Gayle Buller

IPC: C07D311/00 ,  G01N1/30 ,  C07D405/12 ,  C07D405/14 ,  G01N33/50

Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

公开(公告)号：US10246410B2

公开(公告)日：2019-04-02

申请号：US15529437

申请日：2015-12-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wenjun Zhou ,  Kyle Gee ,  Yolanda Tennico ,  Peter Slade ,  Hee Chol Kang ,  Shaheer Khan ,  Brian Evans ,  James Stray

IPC: C07C309/47 ,  G01N33/58 ,  C07D311/84 ,  C07C309/51

Abstract: The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.

公开(公告)号：US10087280B2

公开(公告)日：2018-10-02

申请号：US15491650

申请日：2015-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Xin Wang ,  Hee Chol Kang ,  Aimei Chen ,  Yexin Wu ,  Yi-Zhen Hu ,  Robert Aggeler ,  Quentin Low

IPC: C08G61/12 ,  C07F7/08 ,  C08L65/00 ,  G01N33/533 ,  G01N33/58 ,  H01L51/00 ,  C09K11/06 ,  G01N33/545

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US09624424B2

公开(公告)日：2017-04-18

申请号：US14566166

申请日：2014-12-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen Hu ,  Hee Chol Kang

IPC: C07D403/06 ,  C07D413/06 ,  C07D417/06 ,  C09K11/06 ,  C07D209/14 ,  C07D401/14 ,  C09B23/01 ,  C09B23/08 ,  G01N33/52 ,  C07D209/26 ,  C07D401/10 ,  C07H19/207

CPC classification number: C07H19/207 ,  C07D209/14 ,  C07D209/26 ,  C07D401/10 ,  C07D401/14 ,  C09B23/0041 ,  C09B23/0066 ,  C09B23/086 ,  C09B23/164 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1044 ,  C09K2211/1059 ,  C09K2211/1074 ,  G01N33/52

Abstract: Provided herein are heptamethine cyanine dyes having a large Stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large Stokes shift dyes as fluorescence resonance energy transfer (FRET) acceptors or donors.

公开(公告)号：US20150219634A1

公开(公告)日：2015-08-06

申请号：US14615818

申请日：2015-02-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Michael Janes ,  Iain Johnson ,  Hee Chol Kang

IPC: G01N33/52 ,  C07D491/06

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

Abstract translation: 描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。 该化合物可用于检测活细胞中氧化应激和相关过程。

公开(公告)号：US20130288911A1

公开(公告)日：2013-10-31

申请号：US13793517

申请日：2013-03-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Aimei Chen ,  Kyle Gee ,  Hee Chol Kang

IPC: G01N21/64

CPC classification number: G01N33/64 ,  C07D311/82 ,  C07D471/06 ,  C07F5/022 ,  C07F9/5442 ,  C07F9/65522 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/10 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1033 ,  C09K2211/1055 ,  C09K2211/1088 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/50 ,  G01N33/52 ,  G01N2021/6439 ,  Y10T436/143333

Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.

Abstract translation: 本公开涉及能够检测含有醛和酮基的分析物的荧光席夫碱形成染料。 染料含有亲核肼基附属物，并能够原位结合和检测分析物。

公开(公告)号：US10739330B2

公开(公告)日：2020-08-11

申请号：US16002991

申请日：2018-06-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle Gee ,  Hee Chol Kang ,  Wenjun Zhou ,  Bradley Dubbels ,  Michael Olszowy ,  Michael O'Grady

IPC: G01N33/50 ,  C09B11/28 ,  C07K16/28 ,  C07C309/61 ,  C07D471/04

Abstract: Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the UV/VIS spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.

公开(公告)号：US10006918B2

公开(公告)日：2018-06-26

申请号：US15176872

申请日：2016-06-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Aimei Chen ,  Kyle Gee ,  Hee Chol Kang

IPC: C07F5/02 ,  C09B11/24 ,  G01N21/64 ,  G01N33/50 ,  G01N33/64 ,  C07D311/82 ,  C07D471/06 ,  C07F9/54 ,  C07F9/655 ,  G01N33/52 ,  C09K11/06

CPC classification number: G01N33/64 ,  C07D311/82 ,  C07D471/06 ,  C07F5/022 ,  C07F9/5442 ,  C07F9/65522 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/10 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1033 ,  C09K2211/1055 ,  C09K2211/1088 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/50 ,  G01N33/52 ,  G01N2021/6439 ,  Y10T436/143333

Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.

公开(公告)号：US09518981B2

公开(公告)日：2016-12-13

申请号：US14384843

申请日：2013-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yi-Zhen Hu ,  Aimei Chen ,  Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli

IPC: C07D215/00 ,  G01N33/52 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/84 ,  C07D417/06

CPC classification number: G01N33/84 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C09B23/06 ,  G01N33/52

Abstract: Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

Abstract translation: 本文公开了通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原性核酸结合花青染料，其染料是用于检测ROS的探针，并测定体外和/或体内细胞中的氧化应激。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。

公开(公告)号：US09422384B2

公开(公告)日：2016-08-23

申请号：US14745815

申请日：2015-06-22

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Hofslokken Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven M. Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/10 ,  C08F230/08 ,  C07F7/18 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.