公开(公告)号：US20110045503A1

公开(公告)日：2011-02-24

申请号：US12666256

申请日：2008-07-24

Applicant: Kyle Gee ,  Michael Janes ,  Iain Johnson ,  Hee Chol Kang

Inventor： Kyle Gee ,  Michael Janes ,  Iain Johnson ,  Hee Chol Kang

IPC: G01N33/52 ,  C07D471/06

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

Abstract translation: 描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。 该化合物可用于检测活细胞中氧化应激和相关过程。

公开(公告)号：US20230333097A1

公开(公告)日：2023-10-19

申请号：US17855993

申请日：2022-07-01

Applicant: ZHEJIANG UNIVERSITY

Inventor： Qing YE ,  Jianhua MAO ,  Dandan TIAN

IPC: G01N33/543 ,  G01N33/544 ,  G01N33/68

CPC classification number: G01N33/54306 ,  G01N33/54326 ,  G01N33/544 ,  G01N33/6893 ,  G01N33/6854 ,  G01N2333/4701 ,  G01N2800/347

Abstract: The present disclosure provides a kit for detecting an anti-vinculin-immunoglobulin G (IgG) antibody, including the antigen protein vinculin, a solid phase carrier, a labeled antibody, an antigen diluent, a sample dilution buffer, an antibody diluent, a substrate color development reagent, a washing solution, a standard, a positive quality control, and negative quality control. In the present disclosure, the kit can detect the anti-Vinculin-IgG antibody in a sample to be tested by indirect reaction combined with magnetic particle-based chemiluminescence immunoassay. Autoantibodies against the target antigen vinculin are identified in the serum of a patient with autoimmune nephrotic syndrome for the first time, and a detection kit is provided for the autoantibodies. The present disclosure provides a basis for molecular mechanism research and clinical diagnosis and treatment of autoimmune nephrotic syndrome related to vinculin and vinculin-IgG autoantibodies at home and abroad.

公开(公告)号：US20150219634A1

公开(公告)日：2015-08-06

申请号：US14615818

申请日：2015-02-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Michael Janes ,  Iain Johnson ,  Hee Chol Kang

IPC: G01N33/52 ,  C07D491/06

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

Abstract translation: 描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。 该化合物可用于检测活细胞中氧化应激和相关过程。

公开(公告)号：US07507555B2

公开(公告)日：2009-03-24

申请号：US10554194

申请日：2004-03-15

Applicant: Yoshio Umezawa ,  Moritoshi Sato

Inventor： Yoshio Umezawa ,  Moritoshi Sato

IPC: C12N9/12 ,  C12Q1/48 ,  G01N33/52

CPC classification number: G01N33/542 ,  G01N33/92 ,  G01N2333/4701 ,  Y10T436/143333

Abstract: The present invention provides probes for detection and quantification of a lipid second messenger, which comprises: a polypeptide specifically bound to the lipid second messenger; two chromophores respectively having different fluorescence wavelengths, wherein each of the chromophores is linked to each end of the polypeptide through a rigid linker sequence; and a membrane localization sequence linked to one of the chromophores through a rigid linker sequence. According to the present invention, it is now possible to quantitatively detect when and in which site of a living cell the lipid second messengers are produced.

Abstract translation: 本发明提供了用于检测和定量脂质第二信使的探针，其包括：特异性结合于脂质第二信使的多肽; 两个发色团分别具有不同的荧光波长，其中每个发色团通过刚性接头序列连接到多肽的每个末端; 以及通过刚性接头序列与发色团之一连接的膜定位序列。 根据本发明，现在可以定量地检测生物体脂质第二信使的活细胞的何时和哪个部位。

公开(公告)号：US20150254421A1

公开(公告)日：2015-09-10

申请号：US14717453

申请日：2015-05-20

Applicant: Washington University

Inventor： Randall Bateman ,  Bruce W. Patterson ,  Donald L. Elbert

IPC: G06F19/00 ,  A61B5/00

CPC classification number: G16H50/50 ,  A61B5/4088 ,  G01N33/6896 ,  G01N2333/4701 ,  G01N2800/2821

Abstract: A method of diagnosing an amyloid pathology in the central nervous system of a patient using measurements of enrichment kinetics of at least one amyloid-β isoform is provided. In addition, a model to predict enrichment kinetics of at least one amyloid-β isoform, methods of calibrating the model, and methods of using the model to diagnosing an amyloid pathology in the central nervous system of a patient are provided.

Abstract translation: 使用测量至少一种淀粉样蛋白的富集动力学来诊断患者的中枢神经系统中的淀粉样蛋白病理学的方法; 提供同种型。 此外，一种预测至少一种淀粉样蛋白的富集动力学的模型 提供了校准模型的方法，以及使用该模型诊断患者中枢神经系统中的淀粉样蛋白病理学的方法。

公开(公告)号：US20120040389A1

公开(公告)日：2012-02-16

申请号：US13185774

申请日：2011-07-19

Applicant: François Ichas ,  Francesca De Giorgi Ichas ,  Pier-Vincenzo Piazza ,  Jean Dessolin ,  Laura Schembri ,  Flora Tomasello ,  Lydia Lartigue

Inventor： François Ichas ,  Francesca De Giorgi Ichas ,  Pier-Vincenzo Piazza ,  Jean Dessolin ,  Laura Schembri ,  Flora Tomasello ,  Lydia Lartigue

IPC: C12Q1/37

CPC classification number: G01N33/5008 ,  C07K14/4747 ,  C07K2319/60 ,  G01N33/5005 ,  G01N33/502 ,  G01N33/573 ,  G01N33/68 ,  G01N33/6845 ,  G01N2333/4701 ,  G01N2510/00

Abstract: The invention relates to a method for demonstration of the occurrence of a molecular event, particularly in a cell, characterised by the detection of the ‘solubilisation’ of a fixed protein marker (or the fixing of a solubilised protein marker) which is a direct or indirect marker for the occurrence of the particular molecular event. Said protein marker is present in the cell before the above detection, the cell being subjected to a permeabilisation of the plasma membrane before detection, which liberates the solubilised protein into the extracellular medium, the presence of the marker protein thus being detected in the cell or the extra-cellular medium by any appropriate means, which permits the detection of whether the solubilisation, or the fixing have taken place and, hence, the corresponding molecular event.

Abstract translation: 本发明涉及用于证明分子事件的发生，特别是在细胞中的发生的方法，其特征在于检测固定的蛋白质标记物（或溶解的蛋白质标记物的固定）的“溶解”，其是直接或 发生特定分子事件的间接标记。 所述蛋白质标记物在上述检测之前存在于细胞中，所述细胞在检测前进行质膜渗透，其将溶解的蛋白质释放到细胞外培养基中，因此在细胞中检测到标志蛋白的存在或 通过任何适当方式的细胞外介质，其允许检测是否发生溶解或固定，因此检测相应的分子事件。

公开(公告)号：US20070009882A1

公开(公告)日：2007-01-11

申请号：US10558191

申请日：2004-05-27

Applicant: Lawrence Rosenberg

Inventor： Lawrence Rosenberg

IPC: C12N5/08 ,  C12Q1/00

CPC classification number: C12N5/0676 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2506/22 ,  C12N2533/54 ,  G01N33/5008 ,  G01N33/5017 ,  G01N33/5023 ,  G01N33/5026 ,  G01N33/507 ,  G01N2333/4701 ,  G01N2400/36

Abstract: The present invention relates to an in vitro method for screening agents inducing islet cell neogenesis or duct-to-islet cell transdifferentiation, which comprises the steps of: a) expanding in vitro cells of a duct-like structure obtained by inducing cystic formation in cells in or associated with post-natal islets of Langerhans; b) treating said expanded cells of said duct-like structure with an agent screened; and c) determining potency of said agent of inducing islet cell differentiation of said duct-like structure in becoming insulin-producing cells.

Abstract translation: 本发明涉及一种诱导胰岛细胞新生或胰岛细胞转分化的筛选剂的体外方法，其包括以下步骤：a）通过在细胞中诱导囊性形成获得的管状结构扩增体外细胞 在兰格汉斯的产后胰岛内或与之相关; b）用筛选的试剂处理所述管状结构的所述膨胀的细胞; 以及c）确定所述诱导胰岛细胞分化的药剂在变成胰岛素产生细胞时的效力。

公开(公告)号：US20060265764A1

公开(公告)日：2006-11-23

申请号：US10554194

申请日：2004-03-15

Applicant: Yoshio Umezawa ,  Moritoshi Sato

Inventor： Yoshio Umezawa ,  Moritoshi Sato

IPC: A01K67/00 ,  C12Q1/68 ,  G01N33/567

CPC classification number: G01N33/542 ,  G01N33/92 ,  G01N2333/4701 ,  Y10T436/143333

Abstract: The present invention provides probes for detection and quantification of a lipid second messenger, which comprises: a polypeptide specifically bound to the lipid second messenger; two chromophores respectively having different fluorescence wavelengths, wherein each of the chromophores is linked to each end of the polypeptide through a rigid linker sequence; and a membrane localization sequence linked to one of the chromophores through a rigid linker sequence. According to the present invention, it is now possible to quantitatively detect when and in which site of a living cell the lipid second messengers are produced.

公开(公告)号：US20160334393A1

公开(公告)日：2016-11-17

申请号：US15221230

申请日：2016-07-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Michael JANES ,  Iain JOHNSON ,  Hee Chol KANG

IPC: G01N33/52 ,  G01N33/58 ,  C07F9/54 ,  C07D491/16 ,  C07K5/02

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

公开(公告)号：US08981100B2

公开(公告)日：2015-03-17

申请号：US12666256

申请日：2008-07-24

Applicant: Hee Chol Kang ,  Kyle Gee ,  Iain Johnson ,  Michael Janes

Inventor： Hee Chol Kang ,  Kyle Gee ,  Iain Johnson ,  Michael Janes

IPC: C07D491/16 ,  C07D491/00 ,  G01N33/52

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

Abstract translation: 描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。 该化合物可用于检测活细胞中氧化应激和相关过程。