公开(公告)号：US20110045503A1

公开(公告)日：2011-02-24

申请号：US12666256

申请日：2008-07-24

Applicant: Kyle Gee ,  Michael Janes ,  Iain Johnson ,  Hee Chol Kang

Inventor： Kyle Gee ,  Michael Janes ,  Iain Johnson ,  Hee Chol Kang

IPC: G01N33/52 ,  C07D471/06

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

Abstract translation: 描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。 该化合物可用于检测活细胞中氧化应激和相关过程。

公开(公告)号：US08981100B2

公开(公告)日：2015-03-17

申请号：US12666256

申请日：2008-07-24

Applicant: Hee Chol Kang ,  Kyle Gee ,  Iain Johnson ,  Michael Janes

Inventor： Hee Chol Kang ,  Kyle Gee ,  Iain Johnson ,  Michael Janes

IPC: C07D491/16 ,  C07D491/00 ,  G01N33/52

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.

Abstract translation: 描述了与结合硫醇化合物的亲电子反应性基团取代的荧光喹诺酮香豆素化合物。 该化合物可用于检测活细胞中氧化应激和相关过程。

公开(公告)号：US08772487B2

公开(公告)日：2014-07-08

申请号：US12864038

申请日：2009-01-23

Applicant: Aimei Chen ,  Kyle Gee ,  Hee Chol Kang

Inventor： Aimei Chen ,  Kyle Gee ,  Hee Chol Kang

IPC: C07D471/06 ,  C07F9/655

CPC classification number: G01N33/64 ,  C07D311/82 ,  C07D471/06 ,  C07F5/022 ,  C07F9/5442 ,  C07F9/65522 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/10 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1033 ,  C09K2211/1055 ,  C09K2211/1088 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/50 ,  G01N33/52 ,  G01N2021/6439 ,  Y10T436/143333

Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.

Abstract translation: 本公开涉及能够检测含有醛和酮基的分析物的荧光席夫碱形成染料。 染料含有亲核肼基附属物，并能够原位结合和检测分析物。

公开(公告)号：US20130287689A1

公开(公告)日：2013-10-31

申请号：US13881672

申请日：2011-11-01

Applicant: Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli ,  Lai-Qiang Ying ,  Bruce Branchaud

Inventor： Hee Chol Kang ,  Kyle Gee ,  Bhaskar Mandavilli ,  Lai-Qiang Ying ,  Bruce Branchaud

IPC: G01N21/64 ,  C09B57/00

CPC classification number: G01N21/6486 ,  C09B23/06 ,  C09B23/08 ,  C09B23/083 ,  C09B57/00

Abstract: The present invention is directed to compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced dyes, including Cy-based hydrocyanine dyes and Cy-based deuterocyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and/or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

Abstract translation: 本发明涉及通过常规荧光显微镜，荧光光谱，流式细胞术和/或高含量成像检测活性氧（ROS）的化合物，组合物，方法和试剂盒。 本文公开的化合物是新的还原染料，包括基于Cy的氢化花青染料和Cy-基氘异氰染料，其染料是用于检测ROS的探针和测量体外和/或体内细胞中的氧化应激。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的新型还原染料即ROS探针的方法。

公开(公告)号：US09145361B2

公开(公告)日：2015-09-29

申请号：US14006129

申请日：2012-03-22

Applicant: Kyle Gee ,  Aimei Chen ,  Hee Chol Kang

Inventor： Kyle Gee ,  Aimei Chen ,  Hee Chol Kang

IPC: G01N33/532 ,  C07C309/42 ,  C07D225/02 ,  C07D225/08 ,  C07K16/18 ,  C07D223/08 ,  C07D223/26 ,  C07K1/13

CPC classification number: C07D225/08 ,  C07C309/42 ,  C07C2603/36 ,  C07D223/08 ,  C07D223/26 ,  C07D225/02 ,  C07K1/13 ,  C07K16/18 ,  G01N33/532

Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.

Abstract translation: 本公开涉及用于将点击反应性基团与载体分子或固体支持物缀合的反应性酯试剂。 反应性酯剂具有通式IA，其中在整个申请中描述了变量R1，R2，R3，Ra和L。

公开(公告)号：US20100291547A1

公开(公告)日：2010-11-18

申请号：US12864038

申请日：2009-01-23

Applicant: Aimei Chen ,  Kyle Gee ,  Hee Chol Kang

Inventor： Aimei Chen ,  Kyle Gee ,  Hee Chol Kang

IPC: C12Q1/02 ,  C07D311/82 ,  C07F9/54 ,  C07K14/00 ,  C07H19/04 ,  C07H21/00 ,  G01N21/76 ,  G01N33/50 ,  G01N33/68 ,  C12Q1/70 ,  C12Q1/68

CPC classification number: G01N33/64 ,  C07D311/82 ,  C07D471/06 ,  C07F5/022 ,  C07F9/5442 ,  C07F9/65522 ,  C09B11/24 ,  C09K11/06 ,  C09K2211/10 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1033 ,  C09K2211/1055 ,  C09K2211/1088 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/50 ,  G01N33/52 ,  G01N2021/6439 ,  Y10T436/143333

Abstract: The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.

Abstract translation: 本公开涉及能够检测含有醛和酮基的分析物的荧光席夫碱形成染料。 染料含有亲核肼基附属物，并能够原位结合和检测分析物。

公开(公告)号：US20140107323A1

公开(公告)日：2014-04-17

申请号：US14006129

申请日：2012-03-22

Applicant: Kyle Gee ,  Aimei Chen ,  Hee Chol Kang

Inventor： Kyle Gee ,  Aimei Chen ,  Hee Chol Kang

IPC: C07C309/42 ,  C07D223/08 ,  C07K1/13 ,  C07D223/26

CPC classification number: C07D225/08 ,  C07C309/42 ,  C07C2603/36 ,  C07D223/08 ,  C07D223/26 ,  C07D225/02 ,  C07K1/13 ,  C07K16/18 ,  G01N33/532

Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.

Abstract translation: 本公开涉及用于将点击反应性基团与载体分子或固体支持物缀合的反应性酯试剂。 反应性酯剂具有通式IA，其中在整个申请中描述了变量R1，R2，R3，Ra和L。

公开(公告)号：US5846737A

公开(公告)日：1998-12-08

申请号：US686858

申请日：1996-07-26

Applicant: Hee Chol Kang

Inventor： Hee Chol Kang

IPC: G01N33/533 ,  C07D311/88 ,  C07K16/00

CPC classification number: G01N33/533 ,  Y10S436/80

Abstract: The invention describes useful conjugates of sulforhodamine, wherein the conjugated substance and the fluorophore are separated by an alkanoic acid spacer that is attached to the fluorophore via a sulfonamide bond. The increased length of the covalent linkage due to the spacer results in dye-conjugates having a number of surprisingly advantageous properties relative to previous sulforhodamine-labeled conjugates, including increased fluorescence.

Abstract translation: 本发明描述了磺酰罗丹胺的有用缀合物，其中共轭物质和荧光团通过经由磺酰胺键连接到荧光团上的链烷酸间隔物分离。 由间隔物引起的共价连接的长度增加导致染料缀合物相对于先前的磺酰罗丹明标记的缀合物具有许多令人惊奇的有利性质，包括增加的荧光。

公开(公告)号：US20110313129A1

公开(公告)日：2011-12-22

申请号：US13148913

申请日：2010-02-10

Applicant: Yi-Zhen Hu ,  Hee Chol Kang

Inventor： Yi-Zhen Hu ,  Hee Chol Kang

IPC: C07D403/08 ,  C07D403/14 ,  C07K2/00 ,  C07H21/02 ,  C07H21/04 ,  C07K16/00 ,  C07D401/14 ,  C07H19/207

CPC classification number: C07H19/207 ,  C07D209/14 ,  C07D209/26 ,  C07D401/10 ,  C07D401/14 ,  C09B23/0041 ,  C09B23/0066 ,  C09B23/086 ,  C09B23/164 ,  C09K11/06 ,  C09K2211/1007 ,  C09K2211/1014 ,  C09K2211/1022 ,  C09K2211/1044 ,  C09K2211/1059 ,  C09K2211/1074 ,  G01N33/52

Abstract: Provided herein are heptamethine cyanine dyes having a large Stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large Stokes shift dyes as fluorescence resonance energy transfer (FRET) acceptors or donors.

Abstract translation: 本文提供具有大的斯托克斯位移的七甲基氰花青染料及其盐和共轭物。 还提供了使用和制备这种大的斯托克斯位移染料作为荧光共振能量转移（FRET）受体或供体的方法。

公开(公告)号：US6005113A

公开(公告)日：1999-12-21

申请号：US856422

申请日：1997-05-14

Applicant: Yexin Wu ,  Dieter H. Klaubert ,  Hee Chol Kang ,  Yu-Zhong Zhang

Inventor： Yexin Wu ,  Dieter H. Klaubert ,  Hee Chol Kang ,  Yu-Zhong Zhang

IPC: C09B57/10 ,  C09K11/06 ,  C09K3/00 ,  C09K11/02 ,  G01N33/546

CPC classification number: C09B57/10 ,  C09K11/06 ,  Y10S436/805

Abstract: The invention relates to fluorescent dyes that are substituted or unsubstituted derivatives of 1-(isoindolyl)methylene-isoindole that are bound through both isoindole nitrogens to a boron difluoride moiety, forming a fluorescent dibenzopyrrometheneboron difluoride compound ##STR1## that is further substituted by bathochromic substituents that are aryl or heteroaryl moieties further substituted by an additional aryl or heteroaryl, that is itself optionally further substituted by an additional aryl or heteroaryl. These aryl and heteroaryl groups are separated by a covalent bond, or by an ethenyl, butadienyl or hexatrienyl linkage. The dyes of the invention are particularly useful as labels for carriers, particularly polymeric microparticles. The resulting microparticles have a long-wavelength fluorescence emission, and possess utility for tracing flow in biological systems, particularly in tracing blood flow.

Abstract translation: 本发明涉及通过两个异吲哚氮结合到二氟化硼部分的1-（异吲哚基）亚甲基 - 异吲哚的取代或未取代的衍生物，形成荧光二苯并吡咯并噻唑二氟化物化合物，其进一步被作为芳基的红移取代基取代 或进一步被另外的芳基或杂芳基取代的杂芳基，其本身任选进一步被另外的芳基或杂芳基取代。 这些芳基和杂芳基通过共价键或乙烯基，丁二烯基或己三烯基键分离。 本发明的染料特别可用作载体，特别是聚合物微粒的标签。 所得微粒具有长波长荧光发射，并且具有用于追踪生物系统中的流动的效用，特别是在追踪血流中。