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1.发明授权公开(公告)号：US07811758B2
公开(公告)日：2010-10-12
申请号：US11547093
申请日：2005-03-28
申请人： Toshihiro Mori , Yoshihiko Makino
发明人： Toshihiro Mori , Yoshihiko Makino
CPC分类号： C12N15/1006 , B01D15/14 , B01D15/163 , B01J20/26 , B01J20/262 , B01J20/265 , B01J20/28014 , B01J20/28033 , B01J20/28054 , C12N15/1017 , G01N30/32
摘要： A method of automatically isolating and purifying nucleic acid from a nucleic acid-containing specimen is provided, the method comprising: injecting a liquid into a cartridge for isolation and purification of a nucleic acid including at least two openings from one opening of the at least two openings, in which the cartridge includes a container having the at least two openings and containing a nucleic acid-adsorbent solid phase; passing the liquid through the nucleic acid-adsorbent solid phase by a pressure difference generated by a pressure generation means for generating a pressure difference between the inside and outside of the container; and discharging the liquid from the other opening of the container to the outside of the container by a pressure difference generated by the pressure generation means, wherein a pressure generated in the inside of the container by the pressure generation means is measured, a pressure change velocity and a pressure change acceleration are calculated on the basis of the value of the measured pressure, and the timing of completion of discharge of the liquid from the container is determined by use of a temporal change pattern of at least one of the measured pressure, the pressure change velocity and the pressure change acceleration.
摘要翻译： 提供了一种从含核酸样品自动分离和纯化核酸的方法，所述方法包括：将液体注入用于分离和纯化核酸的液体，所述核酸包括至少两个开口的至少两个开口 开口，其中所述盒包括具有所述至少两个开口并且包含核酸 - 吸附剂固相的容器; 通过由压力产生装置产生的压力差使液体通过核酸 - 吸附剂固相，用于产生容器内部和外部之间的压力差; 并且通过由压力产生装置产生的压力差将液体从容器的另一个开口排出到容器的外部，其中测量由压力产生装置在容器内部产生的压力，压力变化速度 并且基于测量的压力的值来计算压力变化加速度，并且通过使用测量压力中的至少一个的时间变化模式来确定来自容器的液体的排出的完成时间， 压力变化速度和压力变化加速度。
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公开(公告)号：US07321831B2
公开(公告)日：2008-01-22
申请号：US10231242
申请日：2002-08-30
申请人： Yoshihiko Makino , Yoshihiko Abe
发明人： Yoshihiko Makino , Yoshihiko Abe
摘要： It is an object of the present invention to provide a nucleic acid fragment-fixed electrode wherein a probe nucleic acid fragment is fixed on the electrode stably and in an amount-controlled manner. The present invention provides a nucleic acid fragment-fixed electrode wherein a nucleic acid fragment is fixed on the surface of a multi-component self-assembled monolayer of two or more different components which is formed on the electrode, by a covalent bond via a bifunctional linking molecule.
摘要翻译： 本发明的目的是提供一种核酸片段固定电极，其中探针核酸片段稳定地并以量控制的方式固定在电极上。 本发明提供了一种核酸片段固定电极，其中核酸片段固定在多组分自组装单层的两个或多个不同组分的表面上，该组分形成在电极上，通过共价键通过双功能 连接分子。
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3.发明申请公开(公告)号：US20060068491A1
公开(公告)日：2006-03-30
申请号：US11227245
申请日：2005-09-16
申请人： Yoshihiko Makino , Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
发明人： Yoshihiko Makino , Yoshiki Sakaino , Yukio Sudo , Yoshihiko Abe
CPC分类号： B01L3/502753 , B01L3/502707 , B01L3/502715 , B01L2200/0631 , B01L2300/0816 , B01L2300/0867 , B01L2400/0406 , B01L2400/0418 , B01L2400/0487
摘要： A microdevice for performing a method for separating and purifying a nucleic acid, the microdevice comprising: at least one opening; and at least one channel for passing a sample solution, wherein the method comprises: (A) a step of bringing a nucleic acid-containing sample solution into contact with a nucleic acid-adsorbing support having a function of adsorbing a nucleic acid; (B) a step of washing the nucleic acid-adsorbing support with a washing solution in a state of a nucleic acid being adsorbed to the support; and (C) a step of desorbing the nucleic acid from the nucleic acid-adsorbing support by a recovering solution, thereby purifying the nucleic acid; an apparatus for utilizing the microdevice; and a reagent kit for use in the microdevice.
摘要翻译： 一种用于进行分离和纯化核酸的方法的微型装置，所述微型装置包括：至少一个开口; 和用于通过样品溶液的至少一个通道，其中所述方法包括：（A）使含核酸样品溶液与具有吸附核酸功能的核酸吸附载体接触的步骤; （B）在被吸附在所述载体上的核酸的状态下用洗涤液洗涤所述核酸吸附载体的工序; 和（C）通过回收溶液从核酸吸附载体解吸核酸的步骤，从而纯化核酸; 一种利用微型装置的装置; 和用于微型装置的试剂盒。
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公开(公告)号：US20050112658A1
公开(公告)日：2005-05-26
申请号：US10975469
申请日：2004-10-29
申请人： Yoshihiko Makino
发明人： Yoshihiko Makino
CPC分类号： C12Q1/6806 , C07H21/04
摘要： A method of isolating and purifying a nucleic acid, comprises the steps of: (1a) passing a sample solution containing a nucleic acid through a nucleic acid-adsorptive porous membrane to adsorb the nucleic acid to the nucleic acid-adsorptive porous membrane under a specific condition; (2a) passing a wash solution through the nucleic acid-adsorptive porous membrane to wash the nucleic acid-adsorptive porous membrane while adsorbing the nucleic acid under a specific condition; and (3a) passing a elution solution through the nucleic acid-adsorptive porous membrane to desorb the nucleic acid from the nucleic acid-adsorptive porous membrane under a specific condition.
摘要翻译： 分离和纯化核酸的方法包括以下步骤：（1a）使含有核酸的样品溶液通过核酸吸附多孔膜，以将特定的核酸吸附多孔膜吸附到核酸吸附多孔膜上 条件; （2a）使洗涤液通过所述核酸吸附多孔膜，以在特定条件下吸附所述核酸的同时洗涤所述核酸吸附性多孔膜; 和（3a）使洗脱溶液通过核酸吸附多孔膜，以在特定条件下从核酸吸附多孔膜解吸核酸。
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公开(公告)号：US20050095626A1
公开(公告)日：2005-05-05
申请号：US10932138
申请日：2004-09-02
CPC分类号： C12Q1/6806 , B01J2219/00423 , B01J2219/00641 , B01L3/50255 , C12N15/1017 , C12Q1/6837 , G01N1/34 , C12Q2565/501 , C12Q2563/107 , C12Q2531/113 , C12Q2523/308
摘要： The invention provides a rapid, convenient, and automatable method for extracting a highly pure nucleic acid in order to carry out nucleic acid analysis smoothly with high accuracy in an array method. An analyzing method includes analyzing a nucleic acid by an array method, the nucleic acid being separated and purified by a separation and purification method which includes the steps of (a) to (f) identified in the specification.
摘要翻译： 本发明提供一种用于提取高纯度核酸的快速，方便和自动化的方法，以便在阵列方法中以高精度平滑地进行核酸分析。 分析方法包括通过阵列法分析核酸，所述核酸通过分离和纯化方法分离和纯化，所述分离和纯化方法包括说明书中确定的步骤（a）至（f）。
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公开(公告)号：US20050032080A1
公开(公告)日：2005-02-10
申请号：US10732312
申请日：2003-12-11
申请人： Yoshihide Iwaki , Yoshihiko Makino
发明人： Yoshihide Iwaki , Yoshihiko Makino
CPC分类号： C12Q1/6851 , C12Q1/686 , C12Q2537/161 , C12Q2545/107
摘要： An object of the present invention is to provide a method for quantifying a target nucleic acid by PCR. The present invention provides a method for quantifying a target nucleic acid by the polymerase chain reaction utilizing a template nucleic acid comprising a sequence of the target nucleic acid, a pair of primers for amplifying the target nucleic acid, and polymerase, wherein the polymerase chain reaction is conducted in the presence of a pair of competitive primers having a sequence complementary to the sequence of the amplification primer.
摘要翻译： 本发明的目的是提供一种通过PCR定量靶核酸的方法。 本发明提供了使用包含靶核酸序列的模板核酸，用于扩增靶核酸的一对引物和聚合酶通过聚合酶链式反应来定量靶核酸的方法，其中聚合酶链反应 在具有与扩增引物的序列互补的序列的一对竞争性引物的存在下进行。
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公开(公告)号：US06589754B1
公开(公告)日：2003-07-08
申请号：US08946685
申请日：1997-10-08
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
IPC分类号： G01N3353
CPC分类号： G01N33/54386
摘要： An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.
摘要翻译： 一种用于通过测定由高分子聚合物抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析大分子抗原的免疫测定元件。 免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中的大分子抗原的方法。
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公开(公告)号：US06506567B2
公开(公告)日：2003-01-14
申请号：US09774895
申请日：2001-01-31
IPC分类号： C12Q168
CPC分类号： C07D471/06 , C12Q1/6816 , Y10T436/143333 , C12Q2563/173
摘要： A water-soluble fluorescent intercalator compound having the formula: F—La—X F is a fluorescent moiety, X is a divalent cyclic group, and La is a linking group, and at least one of X and La has a site imparting water solubility to the compound or a site that is convertible into a site imparting water solubility to the compound is favorably employable as a fluorescent intercalator in a method for fluorometrically detecting complementary DNA fragments.
摘要翻译： 具有式：F的水溶性荧光嵌入剂化合物是荧光部分，X是二价环状基团，La是连接基团，X和La中的至少一个具有赋予化合物水溶性的部位或 可以转化为赋予化合物水溶性的部位的部位在荧光测定互补DNA片段的方法中有利地用作荧光嵌入剂。
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公开(公告)号：US06368807B2
公开(公告)日：2002-04-09
申请号：US09733365
申请日：2000-12-08
IPC分类号： C12Q168
CPC分类号： C07F17/02
摘要： A compound of the following formula: Ea—La—X—Lb—Eb in which each of Ea and Eb independently is a group having oxidation-reduction activity and having a conjugated system in its group; X is a divalent cyclic group; and each of La and Lb independently is a group which does not form a conjugated system in combination with the conjugated system of each of Ea and Eb and at least one of which has a site imparting water solubility to the compound or a site that is convertible into a site imparting water solubility to the compound, is favorably employable as an electroconductive threading intercalator in an electrochemical method for detecting complementary DNA fragments.
摘要翻译： 下式的化合物：其中Ea和Eb各自独立地是具有氧化还原活性的基团并且在其组中具有共轭体系; X是二价环状基团; 独立地，La和Lb各自是不与Ea和Eb各自的共轭体系结合形成共轭体系的基团，并且其中至少一个具有赋予化合物或可转化位点水溶性的位点 成为赋予化合物水溶性的部位有利地用作检测互补DNA片段的电化学方法中的导电穿线嵌入剂。
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10.发明授权Method of determining calibration curve and analysis method and apparatus using the same 失效确定校准曲线的方法及其分析方法和装置
公开(公告)号：US5948368A
公开(公告)日：1999-09-07
申请号：US947658
申请日：1997-10-09
申请人： Kikuo Hirai , Yoshihiko Makino
发明人： Kikuo Hirai , Yoshihiko Makino
CPC分类号： G01N33/96 , G01N33/48 , Y10S435/967
摘要： One sigmoid calibration curve is split into three parts of low concentration region represented by a high degree function, intermediate concentration region represented by an exponential function and high concentration region represented by a high degree function according to the present invention. The boundary condition of the adjacent two functions is set so that the two functions have an equal slope at the boundary point; thereby, regression functions of the calibration curves in respective regions are found. The number of standard samples for finding a calibration curve can be reduced while the calibration curve found is of high accuracy.
摘要翻译： 一个S形校准曲线被分成由高度函数表示的低浓度区域三个部分，由指数函数表示的中间浓度区域和根据本发明的高度函数表示的高浓度区域。 相邻两个函数的边界条件被设置为使得两个函数在边界点具有相等的斜率; 从而发现各个区域的校准曲线的回归函数。 可以减少用于找到校准曲线的标准样品的数量，同时发现校准曲线具有高精度。
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