公开(公告)号：US20090211240A1

公开(公告)日：2009-08-27

申请号：US12379578

申请日：2009-02-25

申请人： Roland Wayne Patton

发明人： Roland Wayne Patton

IPC分类号： F03B13/18

CPC分类号： F03B13/262 ,  F03B13/1865 ,  F05B2240/93 ,  F05B2260/505 ,  Y02E10/38 ,  Y02E60/17

摘要： Three bodies interact to drive a transmission having a pair of one-way clutches coupling a single length of drive chain or other elongate flexible member to an intermeshed pair of counter rotatable gears so as to drive a primary shaft in a single rotational direction. The three bodies are a fixed body, a floating body, and a suspended and usually submerged weighted body. The three bodies are interconnected by the single length of the elongate flexible member and are spaced apart there along. The weighted body is mounted at one end of the elongate flexible member. The primary shaft is mounted above the surface of a moving fluid mass to either the fixed or floating body. The end of the flexible member opposite to the weight is mounted to the other of the fixed or floating body.

摘要翻译： 三个物体相互作用以驱动具有一对单向离合器的传动装置，该单向离合器将单个长度的驱动链或其它细长的柔性构件连接到一对相互对置的可逆齿轮的啮合，从而沿一个旋转方向驱动主轴。 这三个身体是一个固定的身体，一个浮体，一个悬浮的，通常被淹没的加重体。 三个主体通过细长柔性构件的单个长度相互连接并沿其间隔开。 加重体安装在细长柔性构件的一端。 主轴安装在移动的流体块的表面上方到固定或浮动体上。 柔性构件的与重物相对的端部安装到固定或浮体中的另一个。

公开(公告)号：US07102005B2

公开(公告)日：2006-09-05

申请号：US10428192

申请日：2003-05-02

申请人： Brian Agnew ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Jixiang Liu ,  Vladimir Martin ,  Wayne Patton ,  Thomas Steinberg

发明人： Brian Agnew ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Jixiang Liu ,  Vladimir Martin ,  Wayne Patton ,  Thomas Steinberg

IPC分类号： C07D239/88 ,  C07D277/62 ,  C07D311/82 ,  C07D417/02 ,  C07D495/04

CPC分类号： G01N33/50 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709

摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

公开(公告)号：US20050250152A1

公开(公告)日：2005-11-10

申请号：US11182122

申请日：2005-07-15

申请人： Richard Haugland ,  Thomas Steinberg ,  Wayne Patton ,  Zhenjun Diwu

发明人： Richard Haugland ,  Thomas Steinberg ,  Wayne Patton ,  Zhenjun Diwu

IPC分类号： G01N33/532 ,  C07D239/91 ,  C07D239/94 ,  C07D249/08 ,  C07D263/60 ,  C07D265/22 ,  C07D277/66 ,  C07D401/04 ,  G01N33/58 ,  C12Q1/68 ,  C07H21/04 ,  C08B37/00

CPC分类号： C07D265/22 ,  C07D239/91 ,  Y10T436/143333 ,  Y10T436/200833

摘要： Novel fluorescent derivitization reagents are described that are suitable for coupling to biomolecules that contain aldehyde or ketone functional groups. In one embodiment is provided reagents that have the following formula: wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5 is -L-Z; L is arylene, or a C1-6 perfluoroalkylene, or a single covalent bond; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14 are independently H, C1-6alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-21-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, or sulfo; and R21-R24 are independently H, C1-6 alkyl, C1-6 alkoxy, C1-6 perfluoroalkyl, C1-6 alklyamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The method of treating a sample with the derivativization reagents is described. The reagents are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.

摘要翻译： 描述了适用于与含有醛或酮官能团的生物分子偶联的新型荧光衍生试剂。 在一个实施方案中，提供具有下式的试剂：其中Q是羰基，硫代羰基或磺酰基，R 5是-L-Z; L是亚芳基或C 1-6个全氟亚烷基，或单个共价键; Z是羰基酰肼，酰肼，磺酰肼或硫代羰基酰肼; R 11 -R 14独立地为H，C 1-6烷基，C 1-6烷氧基，C 全氟烷基，C 1-6烷基氨基，二（C 1 -C 21 - 亚烷基）氨基，氨基，羧基，氰基，卤素 ，羟基，硝基，苯基或磺基; R 21和R 24独立地是H，C 1-6烷基，C 1-6烷氧基， C 1-6烷基全氟烷基，C 1-6烷基氨基，二（C 2-12-12 - 烷基）氨基，氨基，羧基，氰基， 卤素，羟基，硝基，苯基，磺基，或-LZ。 描述了用衍生化试剂处理样品的方法。 这些试剂特别可用于在电泳凝胶中标记糖蛋白或糖肽，核酸和脂多糖。

公开(公告)号：US6144415A

公开(公告)日：2000-11-07

申请号：US125320

申请日：1998-08-17

申请人： Steven Wayne Patton ,  Mark Francis Rumreich ,  Donald Henry Willis

发明人： Steven Wayne Patton ,  Mark Francis Rumreich ,  Donald Henry Willis

IPC分类号： H04N5/45 ,  H04N5/44

CPC分类号： H04N5/45

摘要： Apparatus for combining an auxiliary image and a main image includes a source of a main image video signal and a source of samples representing an auxiliary image video signal having successive fields. A quincunx subsampler is coupled to the auxiliary image sample source and subsamples the auxiliary image samples in either a first sample pattern in which samples are taken at a first set of horizontal locations, or a second sample pattern in which samples are taken at a second set of horizontal locations substantially midway between the first set, all in response to a control signal. A signal combiner is coupled to the quincunx subsampler and the main image signal source, and combines the main image signal and a signal representing the quincunx subsampled auxiliary image samples to generate a signal representing a combined image of the main and auxiliary images. A control circuit, generates the quincunx subsampler control signal so that the quincunx subsampler takes samples in one of the sample patterns at the beginning of each auxiliary image video signal field, and in the other sample pattern after a switch time calculated so that the quincunx subsampled auxiliary image samples in the combined image all are taken by the same sample pattern.

摘要翻译： PCT No.PCT / US97 / 04783 Sec。 371日期1998年8月17日第 102（e）日期1998年8月17日PCT 1997年3月6日PCT公布。 第WO97 / 33429号公报 日期1997年9月12日用于组合辅助图像和主图像的装置包括主图像视频信号的源和表示具有连续场的辅助图像视频信号的样本源。 五分之一子采样器耦合到辅助图像采样源，并以辅助图像采样在第一采样图案中采样，其中样本在第一组水平位置拍摄，或第二采样图案，其中采样在第二组 大致位于第一组之间的水平位置，全部是响应于控制信号。 信号组合器耦合到五点二进制器和主图像信号源，并且组合主图像信号和表示五次子采样的辅助图像采样的信号以产生表示主图像和辅助图像的组合图像的信号。 控制电路产生五点二次采样器控制信号，使得五点二次采样器在每个辅助图像视频信号场的开始处采样样本中的一个采样图案，并且在切换时间计算之后的另一采样图案中， 组合图像中的辅助图像样本全部采用相同的样本图案。

公开(公告)号：US5764739A

公开(公告)日：1998-06-09

申请号：US718752

申请日：1996-09-23

申请人： Mark Wayne Patton ,  Douglas Wallace Todd ,  Glenn Albert Davis

发明人： Mark Wayne Patton ,  Douglas Wallace Todd ,  Glenn Albert Davis

IPC分类号： H04M11/00

CPC分类号： H04M11/005 ,  H04M11/002

摘要： A method and apparatus for providing information, such as power consumption, to a subscriber over an electronic network in response to commands provided by the subscriber. The apparatus includes a remote unit which detects and stores the power consumption information, a subscriber device, i.e., a telephone, for generating request commands and a control device which tracks the information. The subscriber using the subscriber device, feeds the request commands via the network to the control device. The control device responds to the commands by directing the remote unit to feed its stored power consumption information onto the network to the control device. The control device then processes the information and indicates the result back to the subscriber.

公开(公告)号：US20110021372A1

公开(公告)日：2011-01-27

申请号：US12821003

申请日：2010-06-22

申请人： Brian AGNEW ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

发明人： Brian AGNEW ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

IPC分类号： C40B30/04 ,  C12Q1/68 ,  G01N33/68 ,  G01N33/566

CPC分类号： G01N33/5008 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709 ,  Y10T436/142222 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/201666

摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

公开(公告)号：US20090162845A1

公开(公告)日：2009-06-25

申请号：US12004842

申请日：2007-12-20

申请人： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Wayne Patton ,  Juan Carcamo

发明人： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Wayne Patton ,  Juan Carcamo

IPC分类号： C12Q1/68 ,  G01N33/566 ,  C07K7/00 ,  C07K16/18 ,  C12P21/04 ,  C07H1/00

CPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6834 ,  G01N33/5308 ,  G01N33/58 ,  G01N33/6854 ,  G01N2458/10 ,  C12Q2565/518 ,  C12Q2563/119 ,  C12Q2565/101

摘要： The present invention concerns compositions and processes that use affinity tags for isolating, and detecting or quantifying analytes, including nucleic acids, proteins and polypeptides. Compositions include nucleic acid compositions and protein compositions with affinity binding pairs, including metal binding peptides and immobilized metals, or peptide affinity groups.

摘要翻译： 本发明涉及使用亲和标签分离和检测或定量分析物（包括核酸，蛋白质和多肽）的组合物和方法。 组合物包括核酸组合物和具有亲和力结合对的蛋白质组合物，包括金属结合肽和固定化金属或肽亲和基团。

公开(公告)号：US20090081722A1

公开(公告)日：2009-03-26

申请号：US12117689

申请日：2008-05-08

申请人： Kyle Gee ,  Courtenay Hart ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

发明人： Kyle Gee ,  Courtenay Hart ,  Wai-Yee Leung ,  Wayne Patton ,  Aleksey Rukavishnikov ,  Richard Haugland ,  Zhenjun Diwu

IPC分类号： G01N1/30 ,  C07F5/02 ,  C07D311/06

CPC分类号： G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

摘要： The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

摘要翻译： 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

公开(公告)号：US20080044804A1

公开(公告)日：2008-02-21

申请号：US11697103

申请日：2007-04-05

申请人： Brian AGNEW ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

发明人： Brian AGNEW ,  Joseph Beechem ,  Kyle Gee ,  Richard Haugland ,  Thomas Steinberg ,  Wayne Patton

IPC分类号： G01N33/00 ,  C12Q1/00 ,  G01N33/20

CPC分类号： G01N33/5008 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709 ,  Y10T436/142222 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/201666

摘要： The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

摘要翻译： 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。

公开(公告)号：US20080026408A1

公开(公告)日：2008-01-31

申请号：US11772739

申请日：2007-07-02

申请人： Wayne Patton ,  Thomas Miller ,  Nan Jiang ,  Michael O'Malley

发明人： Wayne Patton ,  Thomas Miller ,  Nan Jiang ,  Michael O'Malley

IPC分类号： G01N33/566

CPC分类号： G01N33/566 ,  G01N2500/00

摘要： The disclosure features, inter alia, methods and compositions for detecting, isolating, and/or analyzing one or more analytes such as, but not limited to, biomolecules (e.g., proteins, glycoproteins, lipids (e.g., sphingolipids), and carbohydrates). Such methods and compositions are useful in, e.g., the discovery of novel proteins, or the amino acid sequence of novel proteins, or identifying biomarkers for diseases such as cancer, immunological disorders, or microbial infections.

摘要翻译： 本公开尤其涉及用于检测，分离和/或分析一种或多种分析物（例如但不限于）生物分子（例如蛋白质，糖蛋白，脂质（例如鞘脂）和碳水化合物）的方法和组合物。 这些方法和组合物可用于例如新蛋白质的发现，或新蛋白质的氨基酸序列，或识别癌症，免疫学病症或微生物感染等疾病的生物标志物。