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    Pharmaceutical compositions containing pyrylium compounds, pyrylium salts and process for manufacturing a medicament containing the aforesaid compounds
    2.
    发明授权
    Pharmaceutical compositions containing pyrylium compounds, pyrylium salts and process for manufacturing a medicament containing the aforesaid compounds 失效
    含有吡喃鎓化合物，吡喃鎓盐的药物组合物和制备含有上述化合物的药物的方法

    公开(公告)号：US6124342A

    公开(公告)日：2000-09-26

    申请号：US878125

    申请日：1997-06-18

    申请人： Tadashi Okamoto ,  Nobuko Yamamoto ,  Masahiro Kawaguchi

    发明人： Tadashi Okamoto ,  Nobuko Yamamoto ,  Masahiro Kawaguchi

    IPC分类号： C07D335/02 ,  A61K31/33 ,  A61K31/35 ,  A61K31/351 ,  A61K31/38 ,  A61K31/382 ,  A61K41/00 ,  A61P35/00 ,  A61P43/00 ,  C07D309/34 ,  C07D345/00

    CPC分类号： A61K31/382 ,  A61K31/33 ,  A61K31/35 ,  A61K31/38 ,  A61K41/0057

    摘要： The present invention provide pharmaceutical compositions containing a pyrylium compound, a thiopyrylium compound, a selenopyrylium compound or a telluropyrylium compound or a salt of any of the aforesaid compounds as active ingredient. The compounds are selectively absorbed by cancer cells or similar growths in the human or animal body and can be used to bring about distruction of the unwanted growth on irradiation with light of wavelength 600 nm to 1000 nm. The invention also provides a method for the treatment of the human or animal body which comprises administering the compound to a human or animal and irradiating a locus in said animal where the compound is absorbed in order to kill cells at that locus. It further comprises the use of the aforesaid compound for the making of a medicament for use in the treatment of cancer in humans and animals.

    摘要翻译： 本发明提供药物组合物，其含有吡喃鎓化合物，硫代吡喃鎓化合物，硒代吡喃鎓化合物或替硝基吡喃鎓化合物或任何上述化合物的盐作为活性成分。 这些化合物被人类或动物体中的癌细胞或类似的生长选择性地吸收，并且可以用于在600nm至1000nm的波长的光照射下引起不想要的生长的破坏。 本发明还提供了一种治疗人或动物体的方法，其包括将化合物给予人或动物，并照射所述动物中吸收化合物的位点，以杀死该位点处的细胞。 它还包括使用上述化合物制备用于治疗人和动物中的癌症的药物。

    Fluorescent stain containing pyrylium salt and fluorescent staining method of biological sample
    3.
    发明授权
    Fluorescent stain containing pyrylium salt and fluorescent staining method of biological sample 失效
    荧光染料含有吡喃鎓盐和荧光染色法的生物样品

    公开(公告)号：US6022961A

    公开(公告)日：2000-02-08

    申请号：US782798

    申请日：1997-01-13

    申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Yoshinori Tomida ,  Takeshi Miyazaki ,  Masahiro Kawaguchi

    发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Yoshinori Tomida ,  Takeshi Miyazaki ,  Masahiro Kawaguchi

    IPC分类号： G01N33/48 ,  C07D309/34 ,  C07D335/02 ,  C07D345/00 ,  C09B57/00 ,  C09K11/06 ,  C12Q1/68 ,  G01N1/30 ,  G01N33/50 ,  G01N33/52 ,  C07H21/04 ,  C07D335/00

    CPC分类号： G01N33/52 ,  C09B57/00 ,  C12Q1/6816 ,  G01N1/30 ,  C12Q2304/10 ,  C12Q2563/173

    摘要： A stain for a nucleic acid characterized by containing, as an effective component, a pyrylium salt compound having Y as a negative portion and a pyrylium ring or a pyrylium-similar ring, wherein the ring is comprised of X selected from O, S, Se and Te as a hetero atom and has substituents R.sup.1, R.sup.2 and R.sup.3, wherein each of R.sup.1 and R.sup.2 is independently a hydrogen atom, sulfonate group, amino group, substituted or unsubstituted aryl group, etc., R.sup.3 is -A or -L-A, L is -L.sup.1 -, -L.sup.2 -L.sup.3 - or -L.sup.4 -L.sup.5 -L.sup.6 -, and each of L.sup.1 to L.sup.6 is independently --(CH.dbd.CH)--, a divalent group derived from the substituted or unsubstituted aryl group, etc., A is a substituted or unsubstituted aryl group, etc., and Y.sup.- is an anion. A method for detecting a nucleic acid which comprises the steps of reacting a sample with the stain, and then detecting, by an optical means, the double-strand nucleic acid stained with the stain in the case that the double-strand nucleic acid is contained in the sample.

    摘要翻译： 一种核酸的污渍，其特征在于含有Y为负极的吡喃鎓盐化合物作为有效成分，以及吡喃鎓环或吡啶类似的环，其中该环由选自O，S，Se 和Te作为杂原子，并且具有取代基R 1，R 2和R 3，其中R 1和R 2各自独立地为氢原子，磺酸酯基，氨基，取代或未取代的芳基等，R 3为-A或-LA， L为-L1-，-L2-L3-或-L4-L5-L6-，L1至L6各自独立地为 - （CH = CH） - ，衍生自取代或未取代的芳基的二价基团等 A是取代或未取代的芳基等，Y-是阴离子。 一种检测核酸的方法，其包括以下步骤：使样品与染色剂反应，然后通过光学方法检测在含有双链核酸的情况下用染色剂染色的双链核酸 在样品中。

    Partially double-stranded oligonucleotide and method for forming oligonucleotide
    5.
    发明授权
    Partially double-stranded oligonucleotide and method for forming oligonucleotide 失效
    部分双链寡核苷酸和形成寡核苷酸的方法

    公开(公告)号：US5830643A

    公开(公告)日：1998-11-03

    申请号：US974303

    申请日：1992-11-10

    申请人： Nobuko Yamamoto ,  Kinya Kato ,  Harumi Iwashita ,  Masanori Sakuranaga

    发明人： Nobuko Yamamoto ,  Kinya Kato ,  Harumi Iwashita ,  Masanori Sakuranaga

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  C07H21/00 ,  C12P19/34

    CPC分类号： C12N15/10

    摘要： A method for forming an oligonucleotide comprises the steps of synthesizing a pair of oligonucleotides, one oligonucleotide having a base sequence portion comlementary to that of the other at a part of the terminal end region thereof; binding the pair of oligonucleotides at the complementary base sequence portions; and polymerizing nucleic acid bases onto the resulting pair of oligonuceotides. The method may further comprise the step of separating the oligonucleotides thus obtained into single strands.

    摘要翻译： 形成寡核苷酸的方法包括合成一对寡核苷酸的步骤，一个寡核苷酸在其末端区域的一部分具有与另一个寡核苷酸相同的碱基序列部分; 在互补碱基序列部分结合一对寡核苷酸; 并将核酸碱基聚合到所得到的寡核苷酸对上。 该方法还可以包括将由此获得的寡核苷酸分离成单链的步骤。

    Detection method of nucleic acid by use of fluorescent pyrylium stain in intercalation into nucleic acids
    6.
    发明授权
    Detection method of nucleic acid by use of fluorescent pyrylium stain in intercalation into nucleic acids 失效
    通过使用荧光吡啶染色在核酸插入中检测核酸的方法

    公开(公告)号：US5624798A

    公开(公告)日：1997-04-29

    申请号：US170689

    申请日：1993-12-21

    申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Yoshinori Tomida ,  Takeshi Miyazaki ,  Masahiro Kawaguchi

    发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Yoshinori Tomida ,  Takeshi Miyazaki ,  Masahiro Kawaguchi

    IPC分类号： G01N33/48 ,  C07D309/34 ,  C07D335/02 ,  C07D345/00 ,  C09B57/00 ,  C09K11/06 ,  C12Q1/68 ,  G01N1/30 ,  G01N33/50 ,  G01N33/52 ,  C07D309/00 ,  C07D335/00 ,  D06P5/00

    CPC分类号： G01N33/52 ,  C09B57/00 ,  C12Q1/6816 ,  G01N1/30 ,  C12Q2304/10 ,  C12Q2563/173

    摘要： A stain for a nucleic acid characterized by containing, as an effective component, a pyrylium salt compound having Y as a negative portion and a pyrylium ring or a pyrylium-similar ring, wherein the ring is comprised of X selected from O, S, Se and Te as a hetero atom and has substituents R.sup.1, R.sup.2 and R.sup.3, wherein each of R.sup.1 and R.sup.2 is independently a hydrogen atom, sulfonate group, amino group, substituted or unsubstituted aryl group, etc., R.sup.3 is -A or -L-A, L is -L.sup.1 -, -L.sup.2 -L.sup.3 - or -L.sup.4 -L.sup.5 -L.sup.6 -, and each of L.sup.1 to L.sup.6 is independently --(CH.dbd.CH)--, a divalent group derived from the substituted or unsubstituted aryl group, etc., A is a substituted or unsubstituted aryl group, etc., and Y.sup.- is an anion. A method for detecting a nucleic acid which comprises the steps of reacting a sample with the stain, and then detecting, by an optical means, the double-strand nucleic acid stained with the stain in the case that the double-strand nucleic acid is contained in the sample.

    摘要翻译： 一种核酸的污渍，其特征在于含有Y为负极的吡喃鎓盐化合物作为有效成分，以及吡喃鎓环或吡啶类似的环，其中该环由选自O，S，Se 和Te作为杂原子，并且具有取代基R 1，R 2和R 3，其中R 1和R 2各自独立地为氢原子，磺酸酯基，氨基，取代或未取代的芳基等，R 3为-A或-LA， L为-L1-，-L2-L3-或-L4-L5-L6-，L1至L6各自独立地为 - （CH = CH） - ，衍生自取代或未取代的芳基的二价基团等 A是取代或未取代的芳基等，Y-是阴离子。 一种检测核酸的方法，其包括以下步骤：使样品与染色剂反应，然后通过光学方法检测在含有双链核酸的情况下用染色剂染色的双链核酸 在样品中。

    METHOD FOR PREPARING PROTEIN, DNA, AND RNA FROM CELL
    9.
    发明申请
    METHOD FOR PREPARING PROTEIN, DNA, AND RNA FROM CELL 有权
    从细胞中制备蛋白质，DNA和RNA的方法

    公开(公告)号：US20120053329A1

    公开(公告)日：2012-03-01

    申请号：US13318337

    申请日：2010-05-26

    申请人： Nobuko Yamamoto

    发明人： Nobuko Yamamoto

    IPC分类号： C07K1/34 ,  C07H21/04 ,  C07K1/14

    CPC分类号： C12N15/1017 ,  C07K1/34

    摘要： An object of the present invention is to prepare DNA, RNA, and a protein from one cell and to provide a convenient preparation method with high reproducibility. To prepare a protein, DNA, and RNA from a cell, nuclear and cytoplasmic separation is performed, and a protein, DNA, and RNA are then extracted.

    摘要翻译： 本发明的目的是从一个细胞制备DNA，RNA和蛋白质，并提供方便的制备方法，具有高的再现性。 为了从细胞中制备蛋白质，DNA和RNA，进行核和细胞质分离，然后提取蛋白质，DNA和RNA。