公开(公告)号：US20050214940A1

公开(公告)日：2005-09-29

申请号：US10502224

申请日：2003-01-23

Applicant: Mahendra Rao ,  Tahmina Mujtaba ,  Yuan Wu ,  Ying Liu

Inventor： Mahendra Rao ,  Tahmina Mujtaba ,  Yuan Wu ,  Ying Liu

IPC: A61K35/12 ,  C12N5/0797 ,  A61K45/00 ,  C12N5/08

CPC classification number: C12N5/0622 ,  A61K35/12 ,  A61K35/30 ,  C12N5/0623 ,  G01N33/56966

Abstract: An isolated, pure homogeneous population of mammalian astrocyte retricted precursor cells which is CD44 immunoreactive and which generate astrocytes but not oligodendrocytes is provided. Methods for isolating and using these mammalian astrocyte restricted precursor cells are also provided.

Abstract translation: 提供了分离的，纯均质的哺乳动物星形胶质细胞前体细胞，其是CD44免疫反应性的并且产生星形胶质细胞而不是少突胶质细胞。 还提供了分离和使用这些哺乳动物星形胶质细胞限制性前体细胞的方法。

公开(公告)号：US20050125848A1

公开(公告)日：2005-06-09

申请号：US11036004

申请日：2005-01-14

Applicant: Margot Mayer-Proschel ,  Mahendra Rao ,  Patrick Tresco ,  Darin Messina

Inventor： Margot Mayer-Proschel ,  Mahendra Rao ,  Patrick Tresco ,  Darin Messina

IPC: A61K35/12 ,  C12N5/0797 ,  A01K67/033 ,  C12N5/08 ,  A01K67/00

CPC classification number: A61K35/54 ,  A61K35/12 ,  C12N5/0623

Abstract: A method for isolating human neuroepithelial precursor cells from human fetal tissue by culturing the human fetal cells in fibroblast growth factor and chick embryo extract and immunodepleting from the cultured human fetal cells any cells expressing A2B5, NG2 and eNCAM is provided. In addition, methods for transplanting these cells into an animal are provided. Animals models transplanted with these human neuroepithelial precursor cells and methods for monitoring survival, proliferation, differentiation and migration of the cells in the animal model via detection of human specific markers are also provided.

公开(公告)号：US20050049706A1

公开(公告)日：2005-03-03

申请号：US10941620

申请日：2004-09-14

Applicant: Darrel Brodke ,  Bret Berry ,  Ashok Khandkar ,  Ramaswamy Lakshminarayanan ,  Mahendra Rao

Inventor： Darrel Brodke ,  Bret Berry ,  Ashok Khandkar ,  Ramaswamy Lakshminarayanan ,  Mahendra Rao

IPC: A61F2/00 ,  A61F2/28 ,  A61F2/30 ,  A61F2/44 ,  A61F2/46 ,  A61L27/10 ,  A61L27/30 ,  A61L27/32 ,  A61L27/38 ,  A61L27/56

CPC classification number: A61L27/3804 ,  A61F2/30767 ,  A61F2/442 ,  A61F2/446 ,  A61F2/447 ,  A61F2/4611 ,  A61F2002/2835 ,  A61F2002/30011 ,  A61F2002/30016 ,  A61F2002/30064 ,  A61F2002/30153 ,  A61F2002/30158 ,  A61F2002/30166 ,  A61F2002/30179 ,  A61F2002/30224 ,  A61F2002/30235 ,  A61F2002/30261 ,  A61F2002/30266 ,  A61F2002/30271 ,  A61F2002/3028 ,  A61F2002/30329 ,  A61F2002/30616 ,  A61F2002/30677 ,  A61F2002/30733 ,  A61F2002/30774 ,  A61F2002/30777 ,  A61F2002/30789 ,  A61F2002/3082 ,  A61F2002/30822 ,  A61F2002/30827 ,  A61F2002/3085 ,  A61F2002/30881 ,  A61F2002/30891 ,  A61F2002/30892 ,  A61F2002/30896 ,  A61F2002/3092 ,  A61F2002/30968 ,  A61F2002/4475 ,  A61F2002/4623 ,  A61F2002/4627 ,  A61F2002/4629 ,  A61F2002/4648 ,  A61F2220/0025 ,  A61F2230/0019 ,  A61F2230/0026 ,  A61F2230/0028 ,  A61F2230/0058 ,  A61F2230/0063 ,  A61F2230/0069 ,  A61F2230/0082 ,  A61F2250/0019 ,  A61F2250/0023 ,  A61F2310/00203 ,  A61F2310/00239 ,  A61F2310/00796 ,  A61F2310/00928 ,  A61F2310/0097 ,  A61F2310/00976 ,  A61L27/10 ,  A61L27/30 ,  A61L27/32 ,  A61L27/3608 ,  A61L27/365 ,  A61L27/3847 ,  A61L27/56 ,  A61L2430/02 ,  A61L2430/38

Abstract: An improved bone graft is provided for human implantation, particularly such as a spinal fusion cage for implantation into the inter-vertebral space between two adjacent vertebrae. The improved spinal fusion cage includes a substrate block of high strength biocompatible material having a selected size and shape to fit the anatomical space, and a controlled porosity analogous to natural bone. The substrate block may be coated with a bio-active surface coating material such as hydroxyapatite or a calcium phosphate to promote bone in growth and enhanced bone fusion. Upon implantation, the fusion cage provides a spacer element having a desired combination of mechanical strength together with osteoconductivity and osteoinductivity to promote bone ingrowth and fusion, as well as radiolucency for facilitated post-operative monitoring. The fusion cage may additionally carry one or more natural or synthetic therapeutic agents for further promoting bone ingrowth and fusion.

Abstract translation: 提供改进的骨移植物用于人类植入，特别是例如用于植入到两个相邻椎骨之间的椎间空间中的脊柱融合器。 改进的脊柱融合器包括具有选定尺寸和形状以适应解剖空间的高强度生物相容性材料的基底块，以及类似于天然骨的受控孔隙。 衬底块可以涂覆有生物活性表面涂层材料，例如羟基磷灰石或磷酸钙，以促进骨骼生长和增强骨融合。 植入后，融合笼提供了具有机械强度与骨传导性和骨诱导性的期望组合的间隔元件，以促进骨向内生长和融合，以及便于手术后监测的放射性。 融合笼可另外携带一种或多种天然或合成治疗剂，用于进一步促进骨向内生长和融合。

公开(公告)号：US09334523B2

公开(公告)日：2016-05-10

申请号：US13985559

申请日：2012-02-14

Applicant: Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

Inventor： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

IPC: C12Q1/42 ,  G01N33/50 ,  G01N33/58

CPC classification number: G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

Abstract translation: 本发明涉及用于染色活的干细胞的新的底物和方法。 染色可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

公开(公告)号：US20080216185A1

公开(公告)日：2008-09-04

申请号：US12016415

申请日：2008-01-18

Applicant: Jonathan CHESNUT ,  Antje Taliana ,  Bhaskar Thyagarajan ,  Mahendra Rao ,  Pauline Lieu ,  Robert Bennett ,  Robert Burrier

Inventor： Jonathan CHESNUT ,  Antje Taliana ,  Bhaskar Thyagarajan ,  Mahendra Rao ,  Pauline Lieu ,  Robert Bennett ,  Robert Burrier

IPC: C12N15/00 ,  C12N15/87 ,  C12Q1/68

CPC classification number: C12N15/1086 ,  C12N15/1082

Abstract: The disclosure relates generally to stem cell biology and more specifically to genetic manipulation of stem cells. Methods and compositions using recombinational cloning techniques are disclosed which allow the construction and insertion of complex genetic constructs into embryonic and adult stem cells and progenitor cells. The methods disclosed will allow the harvesting of adult stem cells pre-engineered with integration sites to facilitate early passage genetic modification.

Abstract translation: 本公开一般涉及干细胞生物学，更具体地涉及干细胞的遗传操作。 公开了使用重组克隆技术的方法和组合物，其允许将复杂遗传构建体构建和插入胚胎和成体干细胞和祖细胞中。 所公开的方法将允许收获用整合位点预先设计的成体干细胞，以促进早期遗传修饰。

公开(公告)号：US20070037222A1

公开(公告)日：2007-02-15

申请号：US11487105

申请日：2006-07-14

Applicant: Mahendra Rao ,  Tahmina Naqvi

Inventor： Mahendra Rao ,  Tahmina Naqvi

IPC: G01N33/567 ,  C12N5/08 ,  G01N33/53

CPC classification number: G01N33/5058 ,  A61K35/12 ,  C12N5/0622 ,  C12N5/0623 ,  C12N2501/135 ,  C12N2501/395 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2510/00

Abstract: Glial precursor cell populations from mammalian central nervous system and embryonic stem cells has been isolated. These A2B5+ E-NCAM− glial-restricted precursor (GRP) cells are capable of differentiating into oligodendrocytes, A2B5+ process-bearing astrocytes, and A2B5− fibroblast-like astrocytes, but not into neurons. GRP cells can be maintained by regeneration in culture. GRP cells differ from oligodendrocyte-type-2 astrocyte (0-2A) progenitor cells in growth factor requirements, morphology, and progeny. Methods of use of GRP cells are also disclosed.

Abstract translation: 已经分离了哺乳动物中枢神经系统和胚胎干细胞的胶质前体细胞群。 这些A2B5 +神经胶质细胞前体（GRP）细胞能够分化为少突胶质细胞，携带A2B5 +的星形胶质细胞， 和A2B5 成纤维细胞样星形胶质细胞，但不能进入神经元。 GRP细胞可通过培养中的再生来维持。 GRP细胞不同于少突胶质细胞-2型星形胶质细胞（0-2A）祖细胞的生长因子要求，形态和后代。 还公开了使用GRP细胞的方法。

公开(公告)号：US20050177238A1

公开(公告)日：2005-08-11

申请号：US11040477

申请日：2005-01-20

Applicant: Ashok Khandkar ,  Bret Berry ,  Darrel Brodke ,  Ramaswamy Lakshminarayanan ,  Mahendra Rao

Inventor： Ashok Khandkar ,  Bret Berry ,  Darrel Brodke ,  Ramaswamy Lakshminarayanan ,  Mahendra Rao

IPC: A61F2/00 ,  A61F2/28 ,  A61F2/30 ,  A61F2/44 ,  A61F2/46 ,  A61L27/10 ,  A61L27/30 ,  A61L27/32 ,  A61L27/38 ,  A61L27/56

CPC classification number: A61F2/30767 ,  A61F2/442 ,  A61F2/446 ,  A61F2/447 ,  A61F2/4611 ,  A61F2002/2835 ,  A61F2002/30011 ,  A61F2002/30016 ,  A61F2002/30064 ,  A61F2002/30153 ,  A61F2002/30158 ,  A61F2002/30166 ,  A61F2002/30179 ,  A61F2002/30224 ,  A61F2002/30235 ,  A61F2002/30261 ,  A61F2002/30266 ,  A61F2002/30271 ,  A61F2002/3028 ,  A61F2002/30329 ,  A61F2002/30616 ,  A61F2002/30677 ,  A61F2002/30733 ,  A61F2002/30774 ,  A61F2002/30777 ,  A61F2002/30789 ,  A61F2002/3082 ,  A61F2002/30822 ,  A61F2002/30827 ,  A61F2002/3085 ,  A61F2002/30881 ,  A61F2002/30891 ,  A61F2002/30892 ,  A61F2002/30896 ,  A61F2002/3092 ,  A61F2002/30968 ,  A61F2002/4475 ,  A61F2002/4623 ,  A61F2002/4629 ,  A61F2002/4648 ,  A61F2220/0025 ,  A61F2230/0019 ,  A61F2230/0026 ,  A61F2230/0028 ,  A61F2230/0058 ,  A61F2230/0063 ,  A61F2230/0069 ,  A61F2230/0082 ,  A61F2250/0019 ,  A61F2250/0023 ,  A61F2310/00203 ,  A61F2310/00239 ,  A61F2310/00796 ,  A61F2310/00928 ,  A61F2310/0097 ,  A61F2310/00976 ,  A61L27/10 ,  A61L27/30 ,  A61L27/32 ,  A61L27/3608 ,  A61L27/3695 ,  A61L27/3856 ,  A61L27/56 ,  A61L2430/02 ,  A61L2430/38

Abstract: An improved bone graft is provided for human implantation, bone graft includes a substrate block of high strength biocompatible material having a selected size and shape to fit the anatomical space, and a controlled porosity analogous to natural bone. The substrate block may be coated with a bio-active surface coating material such as hydroxyapatite or a calcium phosphate to promote bone ingrowth and enhanced bone fusion. Upon implantation, the bone graft provides a spacer element having a desired combination of mechanical strength together with osteoconductivity and osteoinductivity to promote bone ingrowth and fusion, as well as radiolucency for facilitated post-operative monitoring. The bone graft may additionally carry one or more natural or synthetic therapeutic agents for further promoting bone ingrowth and fusion.

Abstract translation: 提供改进的骨移植物用于人类植入，骨移植物包括具有选定尺寸和形状以适应解剖空间的高强度生物相容性材料的基底块，以及类似于天然骨的受控孔隙。 衬底块可以涂覆有生物活性表面涂层材料，例如羟基磷灰石或磷酸钙，以促进骨向内生长和增强骨融合。 植入后，骨移植物提供了具有机械强度与骨传导性和骨诱导性的期望组合的间隔元件，以促进骨向内生长和融合，以及便于术后监测的放射性。 骨移植物还可携带一种或多种天然或合成治疗剂，用于进一步促进骨向内生长和融合。

公开(公告)号：US20050003531A1

公开(公告)日：2005-01-06

申请号：US10911374

申请日：2004-08-04

Applicant: Mahendra Rao ,  Margot Mayer-Proschel ,  Anjali Kalyani

Inventor： Mahendra Rao ,  Margot Mayer-Proschel ,  Anjali Kalyani

IPC: C12N5/02 ,  A61K35/12 ,  A61K35/30 ,  A61K48/00 ,  A61P25/00 ,  A61P25/28 ,  C12N5/0797 ,  C12N5/08

CPC classification number: C12N5/0623 ,  A61K35/12 ,  A61K48/00 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/385

Abstract: A self-renewing restricted stem cell population has been identified in developing (embryonic day 13.5) spinal cords that can differentiate into multiple neuronal phenotypes, but cannot differentiate into glial phenotypes. This neuronal-restricted precursor (NRP) expresses highly polysialated or embryonic neural cell adhesion molecule (E-NCAM) and is morphologically distinct from neuroepithelial stem cells (NEP cells) and spinal glial progenitors derived from embryonic day 10.5 spinal cord. NRP cells self renew over multiple passages in the presence of fibroblast growth factor (FGF) and neurotrophin 3 (NT-3) and express a characteristic subset of neuronal epitopes. When cultured in the presence of RA and the absence of FGF, NRP cells differentiate into GABAergic, glutaminergic, and cholinergic immunoreactive neurons. NRP cells can also be generated from multipotent NEP cells cultured from embryonic day 10.5 neural tubes. Clonal-analysis shows that E-NCAM immunoreactive NRP cells arise from an NEP progenitor cell that generates other restricted CNS precursors. The NEP-derived E-NCAM immunoreactive cells undergo self renewal in defined medium and differentiate into multiple neuronal phenotypes in mass and clonal culture. Thus, a direct lineal relationship exists between multipotential NEP cells and more restricted neuronal precursor cells present in vivo at embryonic day 13.5 in the spinal cord. Methods for treating neurological diseases are also disclosed.

Abstract translation: 在发育（胚胎期13.5）脊髓中已经鉴定出自我更新的限制性干细胞群，其可以分化成多个神经元表型，但不能分化成胶质表型。 这种神经元限制性前体（NRP）表达高度多聚化或胚胎神经细胞粘附分子（E-NCAM），并且在形态学上与神经上皮干细胞（NEP细胞）和源自胚胎第10.5天脊髓的脊髓神经祖细胞不同。 NRP细胞在成纤维细胞生长因子（FGF）和神经营养因子3（NT-3）的存在下自我更新多次，并表达神经元表位的特征亚群。 当在RA和无FGF的存在下培养时，NRP细胞分化为GABA能，谷氨酸能和胆碱能免疫反应性神经元。 NRP细胞还可以从从胚胎第10.5天神经管培养的多能NEP细胞产生。 克隆分析表明，E-NCAM免疫反应性NRP细胞来自产生其他受限制的CNS前体的NEP祖细胞。 NEP衍生的E-NCAM免疫反应性细胞在限定的培养基中进行自我更新，并在大量和克隆培养中分化成多个神经元表型。 因此，多潜能NEP细胞与脊髓中第13.5天体内存在的更多受限的神经元前体细胞之间存在直接的直线关系。 还公开了治疗神经疾病的方法。

公开(公告)号：US20130323770A1

公开(公告)日：2013-12-05

申请号：US13985559

申请日：2012-02-14

Applicant: Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

Inventor： Uma Lakshmipathy ,  Upinder Singh ,  Scott Grecian ,  Rene Quintanilla ,  Kyle Gee ,  Mahendra Rao

IPC: C12Q1/42

CPC classification number: G01N33/5005 ,  C09B11/24 ,  C09B19/00 ,  C09B21/00 ,  C09B57/00 ,  C09B57/02 ,  C12Q1/42 ,  C12Y301/03001 ,  G01N1/30 ,  G01N33/5073 ,  G01N33/581 ,  G01N33/582 ,  G01N33/586 ,  G01N2001/302 ,  G01N2333/916

Abstract: The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.

Abstract translation: 本发明涉及用于染色活的干细胞的新的底物和方法。 染色可用于在体细胞重编程过程中鉴定诱导的多能干细胞集落。

公开(公告)号：US20130004946A1

公开(公告)日：2013-01-03

申请号：US13448290

申请日：2012-04-16

Applicant: Jonathan Chesnut ,  Bhaskar Thyagarajan ,  Antje Taliana ,  Pauline Lieu ,  Mahendra Rao ,  Robert Bennett ,  Robert Burrier ,  Uma Lakshmipathy ,  Ying Liu

Inventor： Jonathan Chesnut ,  Bhaskar Thyagarajan ,  Antje Taliana ,  Pauline Lieu ,  Mahendra Rao ,  Robert Bennett ,  Robert Burrier ,  Uma Lakshmipathy ,  Ying Liu

IPC: C12N15/90 ,  C12Q1/68

CPC classification number: C12N15/1086 ,  C12N15/1082

Abstract: The disclosure relates generally to stem cell biology and more specifically to genetic manipulation of stem cells. Methods and compositions using recombinational cloning techniques are disclosed which allow the construction and insertion of complex genetic constructs into embryonic and adult stem cells and progenitor cells. The methods disclosed will allow the harvesting of adult stem cells pre-engineered with integration sites to facilitate early passage genetic modification.

Abstract translation: 本公开一般涉及干细胞生物学，更具体地涉及干细胞的遗传操作。 公开了使用重组克隆技术的方法和组合物，其允许将复杂遗传构建体构建和插入胚胎和成体干细胞和祖细胞中。 所公开的方法将允许收获用整合位点预先设计的成体干细胞，以促进早期遗传修饰。