公开(公告)号：US20130122503A1

公开(公告)日：2013-05-16

申请号：US13812242

申请日：2011-07-28

申请人： Junko Yamamoto ,  Tomoo Inden ,  Toshiharu Ohba ,  Tooru Suzuki ,  Hiroyuki Mukai ,  Kiyozo Asada

发明人： Junko Yamamoto ,  Tomoo Inden ,  Toshiharu Ohba ,  Tooru Suzuki ,  Hiroyuki Mukai ,  Kiyozo Asada

IPC分类号： G06F19/12 ,  C12Q1/68

CPC分类号： G06F19/12 ,  C12Q1/6816 ,  C12Q1/6876 ,  C12Q2527/107 ,  G06F19/20

摘要： An object of the present invention is to provide a simple and useful method for producing an RNA-containing probe for detecting a target nucleotide, a simple and useful method, device, and system for processing nucleotide sequence information, and a simple and useful method for detecting a target nucleotide. The present invention provides a method for processing nucleotide sequence information, the method comprising the step of generating partial nucleotide sequences which has 7 to 14 nucleotides and a Tm value of 25 to 40° C. and in which a target nucleotide or a nucleotide adjacent to the target nucleotide is located at a position between 3 and 5 nucleotides from the 3′ or 5′ end. The method according to the present invention is useful for simply and efficiently producing an RNA-containing probe for detecting a target nucleic acid, without the basis of researchers' experiences or guess, and are extremely useful not only in the field of genetic engineering, but also in the field of medical research.

摘要翻译： 本发明的目的在于提供一种用于检测靶核苷酸的含RNA探针的简单且有用的方法，用于处理核苷酸序列信息的简单有用的方法，装置和系统，以及简单有用的方法 检测靶核苷酸。 本发明提供了一种处理核苷酸序列信息的方法，该方法包括产生具有7-14个核苷酸并且Tm值为25至40℃的部分核苷酸序列的步骤，其中靶核苷酸或邻近 靶核苷酸位于3'或5'端3〜5个核苷酸的位置。 根据本发明的方法可用于简单且有效地制备用于检测靶核酸的含RNA探针，而无需基于研究人员的经验或猜测，并且不仅在遗传工程领域中非常有用，而且 也在医学研究领域。

公开(公告)号：US20130065281A1

公开(公告)日：2013-03-14

申请号：US13697665

申请日：2011-05-09

申请人： Yuko Nakabayashi ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

发明人： Yuko Nakabayashi ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

IPC分类号： C12P19/34 ,  C12N9/12

CPC分类号： C12N15/1096 ,  C12P19/34

摘要： A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.

摘要翻译： 一种合成cDNA的方法，其特征在于制备不允许内脱氧核糖核酸酶显示其活性的反应溶液，不脱氧内脱氧核糖核酸酶或去除内切脱氧核糖核酸酶，并进行逆转录反应，其中反应溶液含有处理样品 和逆转录酶，通过用内切核糖核酸酶处理包含RNA和DNA的样品来降解样品中的DNA来形成经处理的样品。 用于合成本发明的cDNA的方法和试剂盒可广泛用于遗传工程领域。

公开(公告)号：US07790849B2

公开(公告)日：2010-09-07

申请号：US12241581

申请日：2008-09-30

申请人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

发明人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

IPC分类号： C07K14/00 ,  C07H21/04

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/505 ,  C07K14/78 ,  C07K2319/00 ,  C12N9/1088 ,  C12N2740/13043 ,  C12N2740/13045

摘要： An isolated polypeptide includes the amino acid sequence shown in SEQ. ID No. 13 and an isolated nucleic acid encoding the polypeptide, such as an isolated nucleic acid including the nucleic acid sequence shown in SEQ. ID No. 17. The isolated polypeptide includes two heparin binding polypeptides connected in tandem and the isolated nucleic acid encodes these. The polypeptide and nucleic acid sequences can improve retroviral vector mediated gene transfer efficiency into target cells.

摘要翻译： 分离的多肽包括SEQ ID NO：1所示的氨基酸序列。 ID号13和编码该多肽的分离的核酸，例如分离的核酸，包括SEQ ID NO：1所示的核酸序列。 分离的多肽包括串联连接的两个肝素结合多肽，分离的核酸编码这些。 多肽和核酸序列可以改进逆转录病毒载体介导的靶细胞的基因转移效率。

公开(公告)号：US20060269999A1

公开(公告)日：2006-11-30

申请号：US11500480

申请日：2006-08-08

申请人： Tomoko Shimojo ,  Hikaru Takakura ,  Kazuyori Ochiai ,  Kiyozo Asada ,  Ikunoshin Kato

发明人： Tomoko Shimojo ,  Hikaru Takakura ,  Kazuyori Ochiai ,  Kiyozo Asada ,  Ikunoshin Kato

IPC分类号： C12P21/06 ,  C07K14/195 ,  C12N15/74 ,  C07H21/04 ,  C12N1/21

CPC分类号： C07K14/195

摘要： Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS:1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in gram positive bacteria.

公开(公告)号：US20060030046A1

公开(公告)日：2006-02-09

申请号：US11181091

申请日：2005-07-14

申请人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

发明人： Kiyozo Asada ,  Takashi Uemori ,  Takashi Ueno ,  Nobuto Koyama ,  Kimikazu Hashino ,  Ikunoshin Kato

IPC分类号： C12N15/867 ,  C07K14/50

CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/505 ,  C07K14/78 ,  C07K2319/00 ,  C12N9/1088 ,  C12N2740/13043 ,  C12N2740/13045

摘要： A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17.

摘要翻译： SEQ ID NO： SEQ ID NO：13，SEQ ID NO： SEQ ID No 30或其功能等同物和SEQ ID NO： ID号17。

公开(公告)号：US06924097B1

公开(公告)日：2005-08-02

申请号：US09368572

申请日：1999-08-05

申请人： Toshiharu Ohba ,  Shuichi Takahashi ,  Yoshiko Anma ,  Kiyozo Asada ,  Ikunoshin Kato

发明人： Toshiharu Ohba ,  Shuichi Takahashi ,  Yoshiko Anma ,  Kiyozo Asada ,  Ikunoshin Kato

IPC分类号： A01H1/00 ,  C12N9/10 ,  C12N15/29 ,  C12N15/82 ,  C12P21/06 ,  C12Q1/68

CPC分类号： C12N15/8223 ,  C12N9/1051

摘要： A plant promoter capable of inducing the expression specifically at the site and stage wherein the reconstitution of plant cell wall xyloglucan is necessary, namely, a plant promoter originating in a gene which encodes an endo-xyloglucan transferase or a gene which encodes a substance having a function equivalent thereto; and a method for modifying the function of a plant with the use of the plant promoter and a method for cloning the plant promoter.

摘要翻译： 需要在植物细胞壁木葡聚糖重构的位点和阶段特异性诱导表达的植物启动子，即来源于编码木糖葡聚糖转移酶的基因的植物启动子或编码具有 功能等同于 以及使用植物启动子修饰植物功能的方法和用于克隆植物启动子的方法。

公开(公告)号：US20050048480A1

公开(公告)日：2005-03-03

申请号：US10333015

申请日：2001-07-18

申请人： Yasuhhiro Tsubosa ,  Kazuhiko Aoyagi ,  Hiroki Sasaki ,  Masaaki Terada ,  Junichi Mineno ,  Kiyozo Asada ,  Ikunoshin Kato

发明人： Yasuhhiro Tsubosa ,  Kazuhiko Aoyagi ,  Hiroki Sasaki ,  Masaaki Terada ,  Junichi Mineno ,  Kiyozo Asada ,  Ikunoshin Kato

IPC分类号： C12N15/12 ,  C12Q1/68 ,  C12Q1/6837 ,  C12Q1/6886

CPC分类号： C12Q1/6886 ,  C12Q1/6837 ,  C12Q2600/158

摘要： To provide a method for selecting a marker gene useful for cancer classification; a method for classifying cancer using the gene; a method for detecting cancer; a kit usable for the classification method or detection method; and a DNA array carrying the gene. According to the present invention, there can be obtained a gene, wherein expression of the above gene is altered independently from genes each of which expression is altered specifically during cell proliferation and expression level of the above gene is specifically altered depending on every type of cancer samples to be tested, whereby the classification or detection of cancer can be carried out conveniently and quickly without giving surgical treatment. Therefore, the present invention is useful for the diagnosis, the treatment, and the like of cancer.

摘要翻译： 提供选择用于癌症分类的标记基因的方法; 使用该基因分类癌症的方法; 一种检测癌症的方法; 可用于分类方法或检测方法的试剂盒; 和携带该基因的DNA阵列。 根据本发明，可以获得上述基因的表达独立于在细胞增殖期间特异性改变的基因的各基因的变化，并且根据每种类型的癌症特异性地改变上述基因的表达水平 待测试的样品，从而可以方便快捷地进行癌症的分类或检测，而无需外科治疗。 因此，本发明对癌症的诊断，治疗等有用。

公开(公告)号：US5744345A

公开(公告)日：1998-04-28

申请号：US489733

申请日：1995-06-14

申请人： Atsushi Shimada ,  Miki Odate ,  Nobuto Koyama ,  Kimikazu Hashino ,  Kiyozo Asada ,  Ikunoshin Kato

发明人： Atsushi Shimada ,  Miki Odate ,  Nobuto Koyama ,  Kimikazu Hashino ,  Kiyozo Asada ,  Ikunoshin Kato

IPC分类号： C12N9/38 ,  C12N15/56

CPC分类号： C12Y302/01023 ,  C12N9/2471

摘要： An isolated hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus is described. Examples include DNA sequences shown in SEQ ID NO. 2 and SEQ ID NO. 4 and DNAs hybridizable with these DNAs. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above sequences or portions thereof is used as a probe or primer is described. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above genes has been introduced is described. A particularly preferred gene encodes an SDS-resistant hyperthermostable .beta.-galactosidase.

摘要翻译： 描述了源自激烈热球菌的分离的超热稳定性β-半乳糖苷酶基因。 实例包括SEQ ID NO： 2和SEQ ID NO。 4和DNA可与这些DNA杂交。 描述了将上述每个上述序列或其部分用作探针或引物的克隆超热稳定性β-半乳糖苷酶基因的方法。 描述了通过培养其中已经引入了含有上述每种基因的质粒的转化体来生产超热稳定性β-半乳糖苷酶的方法。 特别优选的基因编码SDS-抗性超热稳定性β-半乳糖苷酶。

公开(公告)号：US5532337A

公开(公告)日：1996-07-02

申请号：US285440

申请日：1994-08-04

申请人： Ken'ichiro Hayashi ,  Takashi Hashida ,  Kiyozo Asada ,  Hirokazu Kotani ,  Ikunoshin Kato ,  Kenji Sobue

发明人： Ken'ichiro Hayashi ,  Takashi Hashida ,  Kiyozo Asada ,  Hirokazu Kotani ,  Ikunoshin Kato ,  Kenji Sobue

IPC分类号： A61K38/00 ,  A61K31/00 ,  A61P35/00 ,  C07K14/435 ,  C07K14/47 ,  C12N15/09 ,  C12N15/12 ,  C12P21/02 ,  C12R1/19 ,  G01N33/53 ,  C07K14/46 ,  C07K19/00

CPC分类号： C07K14/47

摘要： Provided are functional peptide fragments of human caldesmon having calmodulin and actin-binding activity, the amino acid sequences and nucleic acid sequences therefor.

摘要翻译： 提供具有钙调素和肌动蛋白结合活性的人钙调蛋白的功能性肽片段，其氨基酸序列及其核酸序列。

公开(公告)号：US5516694A

公开(公告)日：1996-05-14

申请号：US381280

申请日：1995-01-31

申请人： Kazuhiko Nishitani ,  Kazuhide Okazawa ,  Kiyozo Asada ,  Ikunoshin Kato

发明人： Kazuhiko Nishitani ,  Kazuhide Okazawa ,  Kiyozo Asada ,  Ikunoshin Kato

IPC分类号： C12N9/10 ,  C12N9/24 ,  C12N9/42 ,  C12N15/82 ,  C12N15/14 ,  C07H21/04

CPC分类号： C12N9/1051 ,  C12N15/8241 ,  C12N15/8261 ,  C12Y204/01207

摘要： Endo-xyloglucan transferases responsible for growth of plant cell wall, genes coding for the enzymes, a method of transferring xyloglucan molecules by using the enzyme, and methods of using the gene are described.