公开(公告)号：US09169482B2

公开(公告)日：2015-10-27

申请号：US13697665

申请日：2011-05-09

申请人： Yuko Nakabayashi ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

发明人： Yuko Nakabayashi ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

IPC分类号： C12P19/34 ,  C12N15/10

CPC分类号： C12N15/1096 ,  C12P19/34

摘要： A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.

摘要翻译： 一种合成cDNA的方法，其特征在于制备不允许内脱氧核糖核酸酶显示其活性的反应溶液，不脱氧内脱氧核糖核酸酶或去除内切脱氧核糖核酸酶，并进行逆转录反应，其中反应溶液含有处理样品 和逆转录酶，通过用内切核糖核酸酶处理包含RNA和DNA的样品来降解样品中的DNA来形成经处理的样品。 用于合成本发明的cDNA的方法和试剂盒可广泛用于遗传工程领域。

公开(公告)号：US20130065281A1

公开(公告)日：2013-03-14

申请号：US13697665

申请日：2011-05-09

申请人： Yuko Nakabayashi ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

发明人： Yuko Nakabayashi ,  Takashi Uemori ,  Hiroyuki Mukai ,  Kiyozo Asada

IPC分类号： C12P19/34 ,  C12N9/12

CPC分类号： C12N15/1096 ,  C12P19/34

摘要： A method for synthesizing cDNA characterized by preparing a reaction solution that does not allow an endodeoxyribonuclease to show its activity, without thermal deactivation of the endodeoxyribonuclease or removal of the endodeoxyribonuclease, and carrying out a reverse transcription reaction, wherein the reaction solution contains a treated sample and a reverse transcriptase, the treated sample being formed by treating a sample comprising RNA and DNA with the endodeoxyribonuclease to degrade DNA in the sample. The method and the kit for synthesizing cDNA of the present invention are widely useful in genetic engineering fields.

摘要翻译： 一种合成cDNA的方法，其特征在于制备不允许内脱氧核糖核酸酶显示其活性的反应溶液，不脱氧内脱氧核糖核酸酶或去除内切脱氧核糖核酸酶，并进行逆转录反应，其中反应溶液含有处理样品 和逆转录酶，通过用内切核糖核酸酶处理包含RNA和DNA的样品来降解样品中的DNA来形成经处理的样品。 用于合成本发明的cDNA的方法和试剂盒可广泛用于遗传工程领域。

公开(公告)号：US20120164654A1

公开(公告)日：2012-06-28

申请号：US13393302

申请日：2010-08-27

申请人： Yuko Nakabayashi ,  Yoshimi Sato ,  Takashi Uemori ,  Hiroyuki Mukai

发明人： Yuko Nakabayashi ,  Yoshimi Sato ,  Takashi Uemori ,  Hiroyuki Mukai

IPC分类号： G01N27/26 ,  C12P19/34 ,  C12N9/12

CPC分类号： C12Q1/686 ,  C12N15/1096 ,  C12Q2527/125 ,  C12Q2521/107

摘要： A composition for a reverse transcription polymerase chain reaction, which comprises a thermostable DNA polymerase, a reverse transcriptase, a dye marker and a specific gravity-increasing agent; and a premix reagent for a one-step RT-PCR, which comprises the composition, is not frozen under usual storage conditions at −20 to −30° C. and has excellent handleability.

摘要翻译： 用于逆转录聚合酶链反应的组合物，其包含热稳定性DNA聚合酶，逆转录酶，染料标记物和比重增加剂; 并且包含该组合物的用于一步RT-PCR的预混合试剂在-20至-30℃的通常储存条件下不冷冻，并且具有优异的可操作性。