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1.发明授权Cultured high-fidelity three-dimensional human urogenital tract carcinomas and process 失效培养高保真三维人体泌尿生殖道癌及其过程
公开(公告)号：US5851816A
公开(公告)日：1998-12-22
申请号：US172962
申请日：1993-12-27
CPC分类号： C12N5/0693 , C12N5/0018 , C12N5/0062 , C12N2500/25 , C12N2500/34 , C12N2500/72 , C12N2525/00 , C12N2531/00
摘要： Artificial high-fidelity three-dimensional human urogenital tract carcinomas are propagated under in vitro-microgravity conditions from carcinoma cells. Artificial high-fidelity three-dimensional human urogenital tract carcinomas are also propagated from a coculture of normal urogenital tract cells inoculated with carcinoma cells. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.
摘要翻译： 人造高保真三维人类泌尿生殖道癌在癌细胞的体外 - 微重力条件下繁殖。 人造高保真三维人类泌尿生殖道癌也从接种癌细胞的正常泌尿生殖道细胞的共培养物繁殖。 微重力培养条件可能是在水平旋转壁培养容器中产生的微重力或模拟微重力。
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2.发明授权
公开(公告)号：US5523228A
公开(公告)日：1996-06-04
申请号：US499348
申请日：1995-07-07
申请人： Marylou Ingram , Glenn F. Spaulding , James J. G. Craft , Chuen P. Ng , Ramez Saroufeem , Geza B. Techy , Ozkan Yazan
发明人： Marylou Ingram , Glenn F. Spaulding , James J. G. Craft , Chuen P. Ng , Ramez Saroufeem , Geza B. Techy , Ozkan Yazan
CPC分类号： C12M27/10 , C12M23/14 , C12M23/24 , Y10S435/818
摘要： A cell culture chamber with a novel hydrodynamic environment, constructed with gas permeable membranes is disclosed. The culture chamber is horizontally rotated, wall movement suspends cells and enhances oxygen mass transfer as cells aggregate and grow into 3-dimensional tissue.
摘要翻译： 公开了一种具有新型流体动力学环境的细胞培养室，由气体渗透膜构成。 培养室水平旋转，壁运动悬浮细胞，增强细胞聚集并生长成3维组织的氧传质。
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3.发明授权
公开(公告)号：US5346990A
公开(公告)日：1994-09-13
申请号：US667974
申请日：1991-03-12
申请人： Glenn F. Spaulding
发明人： Glenn F. Spaulding
CPC分类号： G01N33/689 , A61K35/52 , C07K14/705 , C07K16/28 , G01N2800/367 , Y10S424/811 , Y10S435/806 , Y10S436/906 , Y10S530/852
摘要： A method of sorting living cells based on DNA content. Mammalian sperm subpopulations enriched in X- or Y-sperm. X- and Y-enriched sperm-plasma-membrane vesicles. Substantially pure sex-associated membrane (SAM) proteins. Antibodies binding to X-or Y-SAM proteins, essentially free of antibodies binding to Y- or X-SAM proteins respectively, or to the H-Y antigen. Semen samples enriched for X- or Y-sperm. Methods for increasing the probability that offspring will be male or female comprising the step of allowing as penn from an enriched semen sample to fertilize an ovum. Methods for increasing the probability that offspring will be male or female comprising the step of immunizing a female with X- or Y-SAM proteins. Methods of decreasing fertility comprising the step of immunizing a female with both X- and Y-sperm. Methods of increasing the probability that mammalian offspring will carry a gene for a particular sex-chromosome linked trait.
摘要翻译： 基于DNA含量分选活细胞的方法。 哺乳动物精子亚群富含X或Y精子。 X和Y富集的精子 - 质膜囊泡。 基本纯粹的性相关膜（SAM）蛋白。 与X或Y-SAM蛋白结合的抗体，基本上不含分别与Y-或X-SAM蛋白结合的抗体或H-Y抗原。 精液样品富集X或Y精子。 用于增加后代将是男性或女性的概率的方法包括允许来自富集的精液样品的皮下施用卵子的步骤。 增加后代将是雄性或雌性的概率的方法包括用X或Y-SAM蛋白免疫雌性的步骤。 降低生殖力的方法包括用X和Y精子免疫雌性的步骤。 增加哺乳动物后代携带特定性染色体连锁性状基因的可能性的方法。
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公开(公告)号：US20110015494A1
公开(公告)日：2011-01-20
申请号：US12835386
申请日：2010-07-13
申请人： Glenn F. Spaulding
发明人： Glenn F. Spaulding
CPC分类号： A61B5/02055 , A61B5/0006 , A61B5/0008 , G02B21/0004 , G06F19/00 , G06F19/3418 , G06Q50/22
摘要： A home healthcare management system wherein a patient conducts self diagnoses and self testing, and manages their own medical records at home. A digital microscope is utilized as part of the system that is smaller, of lower cost, faster, of a higher dynamic range, and has a higher resolution than conventional microscopes. The microscope consists of an illumination source, a spatial sub-sampling device and a detector device. The digital microscope provides a vectored method of collecting images from a digital microscope that is independent of the optical resolution, and a slide based coordinate system, and a method of displaying images and communicating such images over the Internet in a file format that does not require a header or prior knowledge of magnification, coordinate system, or tiling structure. The system further includes an interface for physiological monitoring devices and a connection to the Internet for more comprehensive services.
摘要翻译： 家庭医疗管理系统，其中患者进行自我诊断和自检，并在家中管理他们自己的医疗记录。 使用数字显微镜作为系统的一部分，其具有较低的成本，更快的动态范围，并且具有比常规显微镜更高的分辨率。 显微镜由照明源，空间子采样装置和检测器装置组成。 数字显微镜提供了从独立于光学分辨率的数字显微镜收集图像的矢量方法，以及基于幻灯片的坐标系，以及以不需要的文件格式通过因特网显示图像和传送这些图像的方法 标题或放大，坐标系或平铺结构的先前知识。 该系统还包括用于生理监测设备的接口和与互联网的连接以用于更全面的服务。
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公开(公告)号：US5589112A
公开(公告)日：1996-12-31
申请号：US227827
申请日：1994-04-13
申请人： Glenn F. Spaulding
发明人： Glenn F. Spaulding
CPC分类号： C12M27/12 , C12M23/24 , C12M25/16 , Y10S435/818
摘要： An annular culture vessel for growing mammalian cells is constructed in a one piece integral and annular configuration with an open end which is closed by an endcap. The culture vessel is rotatable about a horizontal axis by use of conventional roller systems commonly used in culture laboratories. The end wall of the endcap has tapered access ports to frictionally and sealingly receive the ends of hypodermic syringes. The syringes permit the introduction of fresh nutrient and withdrawal of spent nutrients. The walls are made of conventional polymeric cell culture material and are subjected to neutron bombardment to form minute gas permeable perforations in the walls.
摘要翻译： 用于生长哺乳动物细胞的环形培养容器被构造成一体的整体和环形构造，其开口端由端盖封闭。 培养容器可以通过使用通常用于培养实验室的常规辊系统围绕水平轴线旋转。 端盖的端壁具有锥形进入口，以摩擦和密封地接收皮下注射器的端部。 注射器允许引入新鲜的营养物质和撤回用过的营养物质。 壁由常规的聚合物细胞培养材料制成，并经受中子轰击以在壁中形成微小的透气穿孔。
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公开(公告)号：US08891851B2
公开(公告)日：2014-11-18
申请号：US12835386
申请日：2010-07-13
申请人： Glenn F. Spaulding
发明人： Glenn F. Spaulding
CPC分类号： A61B5/02055 , A61B5/0006 , A61B5/0008 , G02B21/0004 , G06F19/00 , G06F19/3418 , G06Q50/22
摘要： A home healthcare management system wherein a patient conducts self diagnoses and self testing, and manages their own medical records at home. A digital microscope is utilized as part of the system that is smaller, of lower cost, faster, of a higher dynamic range, and has a higher resolution than conventional microscopes. The microscope consists of an illumination source, a spatial sub-sampling device and a detector device. The digital microscope provides a vectored method of collecting images from a digital microscope that is independent of the optical resolution, and a slide based coordinate system, and a method of displaying images and communicating such images over the Internet in a file format that does not require a header or prior knowledge of magnification, coordinate system, or tiling structure. The system further includes an interface for physiological monitoring devices and a connection to the Internet for more comprehensive services.
摘要翻译： 家庭医疗管理系统，其中患者进行自我诊断和自检，并在家中管理他们自己的医疗记录。 使用数字显微镜作为系统的一部分，其具有较低的成本，更快的动态范围，并且具有比常规显微镜更高的分辨率。 显微镜由照明源，空间子采样装置和检测器装置组成。 数字显微镜提供了从独立于光学分辨率的数字显微镜收集图像的矢量方法，以及基于幻灯片的坐标系，以及以不需要的文件格式通过因特网显示图像和传送这些图像的方法 标题或放大，坐标系或平铺结构的先前知识。 该系统还包括用于生理监测设备的接口和与互联网的连接以用于更全面的服务。
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公开(公告)号：US6117674A
公开(公告)日：2000-09-12
申请号：US366065
申请日：1994-12-29
CPC分类号： C12N7/00 , C12N5/0062 , C12N2500/25 , C12N2500/34 , C12N2500/72 , C12N2531/00 , C12N2770/36011
摘要： A process for propagating a pathogen in a three-dimensional tissue mass cultured at microgravity conditions in a culture vessel containing culture media and a culture matrix is provided. The three-dimensional tissue mass is inoculated with a pathogen and pathogen replication in the cells of the tissue mass achieved.
摘要翻译： 提供了在含有培养基和培养基质的培养容器中在微重力条件下培养的三维组织块中繁殖病原体的方法。 在组织块的细胞中用病原体和病原体复制物接种三维组织块。
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8.发明授权Method for producing non-neoplastic, three dimensional, mammalian tissue and cell aggregates under microgravity culture conditions and the products produced therefrom 失效在微重力培养条件下生产非肿瘤，三维哺乳动物组织和细胞聚集体的方法及由其产生的产物
公开(公告)号：US5496722A
公开(公告)日：1996-03-05
申请号：US66292
申请日：1993-05-25
IPC分类号： C12M1/12 , C12M3/04 , C12M3/06 , C12N5/00 , C12N5/02 , A01N1/02 , C12M1/10 , C12M3/02 , C12N5/06 , C12N5/08
CPC分类号： C12N5/0062 , C12M23/24 , C12M27/10 , C12N5/0018 , C12N5/0693 , C12N2500/25 , C12N2500/34 , C12N2500/72 , C12N2525/00 , C12N2531/00
摘要： Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under microgravity culture conditions and form three dimensional cells aggregates with normal cell function. The microgravity culture conditions may be microgravity or simulated microgravity created in a horizontal rotating wall culture vessel.
摘要翻译： 正常的哺乳动物组织和培养过程已经针对三组器官，结构和血液组织开发。 细胞在微重力培养条件下体外培养并形成具有正常细胞功能的三维细胞聚集体。 微重力培养条件可能是在水平旋转墙培养容器中产生的微重力或模拟微重力。
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9.发明授权Method of making a membrane having hydrophilic and hydrophobic surfaces for adhering cells or antibodies by using atomic oxygen or hydroxyl radicals 失效制备具有通过使用原子氧或羟基自由基来粘附细胞或抗体的亲水和疏水表面的膜的方法
公开(公告)号：US5369012A
公开(公告)日：1994-11-29
申请号：US857901
申请日：1992-03-26
IPC分类号： A61L27/28 , A61L33/00 , B01D15/08 , B01D67/00 , B01J20/08 , B01J20/10 , B01J20/32 , B29C59/14 , C12M1/24 , C12M3/04 , C12M3/06 , C12N5/00 , C12P21/00 , G01N30/52 , G01N33/543 , G01N33/545 , C12N11/08 , G01N33/535
CPC分类号： B01D67/009 , A61L27/28 , A61L33/0076 , A61L33/0094 , B01D15/3809 , B01D67/0093 , B01J20/08 , B01J20/103 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/328 , B01J20/3285 , B01J20/3291 , B29C59/14 , C12N5/0068 , C12P21/00 , G01N33/54306 , G01N33/545 , B01D2323/38 , B29L2031/755 , C12N2533/30 , G01N2030/525 , G01N2030/528 , Y10S530/815 , Y10S530/816
摘要： A portion of an organic polymer article such as a membrane is made hydrophilic by exposing a hydrophobic surface of the article to a depth of about 50 to about 5000 angstroms to atomic oxygen or hydroxyl radicals at a temperature below 100.degree. C., preferably below 40.degree. C., to form a hydrophilic uniform surface layer of hydrophilic hydroxyl groups. The atomic oxygen and hydroxyl radicals are generated by a flowing afterglow microwave discharge, and the surface is outside of a plasma produced by the discharge. A membrane having both hydrophilic and hydrophobic surfaces can be used in an immunoassay by adhering antibodies to the hydrophobic surface. In another embodiment, the membrane is used in cell culturing where cells adhere to the hydrophilic surface. Prior to adhering cells, the hydrophilic surface may be grafted with a compatibilizing compound. A plurality of hydrophilic regions bounded by adjacent hydrophobic regions can be produced such that a maximum of one cell per each hydrophilic region adheres.
摘要翻译： 通过在低于100℃，优选低于40℃的温度下将制品的疏水表面暴露于原子氧或羟基自由基的约50至约5000埃的深度，将部分有机聚合物制品（例如膜）制成亲水性 以形成亲水性均匀的亲水羟基表面层。 原子氧和羟基自由基通过流动的余辉微波放电产生，并且表面在通过放电产生的等离子体之外。 具有亲水和疏水表面的膜可以通过将抗体粘附到疏水性表面上而用于免疫测定。 在另一个实施方案中，膜用于细胞培养，其中细胞粘附到亲水表面。 在粘附细胞之前，亲水表面可以用相容化合物接枝。 可以产生由相邻的疏水区域限定的多个亲水区域，使得每个亲水区域最多一个细胞粘附。
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公开(公告)号：US07355698B2
公开(公告)日：2008-04-08
申请号：US11379845
申请日：2006-04-24
申请人： Shishir Shah , Glenn F. Spaulding
发明人： Shishir Shah , Glenn F. Spaulding
IPC分类号： G01N21/03
CPC分类号： G01N21/253 , G01N35/025 , G01N35/028
摘要： An imaging device and system for the high throughput imaging of multi-sample containers is described. Generally speaking, one or more containers capable of holding multiple sample volumes are fixed to a platform which is rotated during image acquisition operations. Images of sample volumes are obtained by moving an imaging device (e.g., a camera) from a first position to a second position across the one or more sample volumes. Because the platform is continuously rotated there is no lost time due to moving the samples (e.g., acceleration, deceleration and backlash compensation). Movement artifacts associated with motion of the imaging device occur in those times when the imaging device is “between” sample volumes, thereby eliminating any lost time due to this motion. Rather than, or in combination with, the aforementioned lights sources, additional light sources may be added to provide epi-illumination.
摘要翻译： 描述了用于多样品容器的高通量成像的成像装置和系统。 一般来说，能够保持多个样本体积的一个或多个容器被固定到在图像采集操作期间旋转的平台。 通过将成像装置（例如，相机）从一个或多个样本体积的第一位置移动到第二位置来获得样本体积的图像。 因为平台连续旋转，所以由于移动样品（例如加速度，减速度和间隙补偿）而不会丢失时间。 在成像装置处于“样品体积之间”的那些时刻，与成像装置的运动相关联的运动伪影发生，从而消除由于该运动引起的任何丢失的时间。 与上述光源不同或组合，可以添加额外的光源以提供外照明。
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