SCAFFOLD-FREE SELF-ORGANIZED 3D SYNTHETIC TISSUE
    83.
    发明申请
    SCAFFOLD-FREE SELF-ORGANIZED 3D SYNTHETIC TISSUE 审中-公开
    无SCAFF自由组织的3D合成组织

    公开(公告)号:US20160256608A1

    公开(公告)日:2016-09-08

    申请号:US15166059

    申请日:2016-05-26

    摘要: The present invention can be used for actual implantation surgery without a scaffold. The present invention provides a synthetic tissue or complex which can be produced by culture and has a high level of differentiation ability. The present invention also provides a therapy and medicament for repairing and/or regenerating tissue using replacement and covering. By culturing cells under specific culture conditions such that medium contains an extracellular matrix synthesis promoting agent, the cells are organized and are easily detached from a culture dish. The present invention was achieved by finding such a phenomenon. In addition, the self contraction of the tissue can be regulated by culturing the tissue in a suspended manner. Therefore, it is possible to regulate the three-dimensional shape of the tissue. The present invention also provides a method for producing an implantable synthetic tissue which does not require a plurality of monolayer cell sheets assembled to form a three-dimensionally structured synthetic tissue. The present invention is characterized by richness in adhesion molecules, nonnecessity of additional fixation at an implantation site, and good biological integration.

    摘要翻译: 本发明可用于没有支架的实际植入手术。 本发明提供可通过培养产生并具有高水平分化能力的合成组织或复合物。 本发明还提供了使用替换和覆盖来修复和/或再生组织的治疗和药物。 通过在特定培养条件下培养细胞,使得培养基含有细胞外基质合成促进剂,细胞被组织并容易从培养皿中分离。 本发明是通过发现这种现象来实现的。 此外,可以通过以悬浮方式培养组织来调节组织的自收缩。 因此,可以调节组织的三维形状。 本发明还提供了一种用于生产可植入的合成组织的方法,其不需要组装成多个单层细胞片以形成三维结构的合成组织。 本发明的特征在于粘附分子的丰富性,植入部位的附加固定的不必要性以及良好的生物整合性。

    CELL CULTURE MEDIUM AND BIOPROCESS OPTIMIZATION
    84.
    发明申请
    CELL CULTURE MEDIUM AND BIOPROCESS OPTIMIZATION 审中-公开
    细胞培养基和生物学优化

    公开(公告)号:US20160251614A1

    公开(公告)日:2016-09-01

    申请号:US15033531

    申请日:2014-11-03

    摘要: The invention provides a chemically defined cell culture media and methods of using the media. The invention also provides an inverse screen to identify small molecules and synergies stimulating proliferation in a chemically defined medium. In this chemical-genetics approach, a compound-protein interaction data-base is used to systematically score genetic targets on a screen-wide scale to extract further information about cell growth. Validated factors were investigated for their ability to maintain cell growth over multiple passages in the chemically defined medium (CDM). Polyamines were identified as important components that enables the CDM to support the long-term maintenance of C1.8 cells and Kc cells (such as Kc167 cells). Our cumulative target scoring approach improves on traditional chemical-genetics methods and is extensible to biological processes in other species.

    摘要翻译: 本发明提供化学上确定的细胞培养基和使用该介质的方法。 本发明还提供了用于鉴定在化学上限定的培养基中刺激增殖的小分子和协同作用的反相屏幕。 在这种化学遗传学方法中,化合物 - 蛋​​白质相互作用数据库用于在屏幕范围内系统地评估基因靶标以提取有关细胞生长的进一步信息。 研究了在化学成分确定的培养基(CDM)中维持多次传代的细胞生长能力的验证因子。 多胺被鉴定为使CDM能够支持C1.8细胞和Kc细胞(如Kc167细胞)的长期维持的重要组成部分。 我们的累积目标评分方法改进了传统的化学遗传学方法,可扩展到其他物种的生物过程。

    CULTURE METHOD FOR PLURIPOTENT STEM CELLS AND KIT AND MEDIUM FOR CULTURE OF PLURIPOTENT STEM CELLS USED THEREIN
    86.
    发明申请
    CULTURE METHOD FOR PLURIPOTENT STEM CELLS AND KIT AND MEDIUM FOR CULTURE OF PLURIPOTENT STEM CELLS USED THEREIN 审中-公开
    用于大肠杆菌干细胞的培养方法和用于其中使用的多发性干细胞培养物的培养基

    公开(公告)号:US20160244728A1

    公开(公告)日:2016-08-25

    申请号:US15065217

    申请日:2016-03-09

    IPC分类号: C12N5/074

    摘要: A culture method for pluripotent stem cells includes obtaining a polypeptide-coated culture surface by applying a polypeptide to a cell culture surface of a support, and culturing pluripotent stem cells by seeding the pluripotent stem cells onto the polypeptide-coated culture surface by using a medium in which the content of an ascorbic acid derivative is equal to or greater than 1.5 mmol/L (mM), in which the polypeptide is (a) a polypeptide having an amino acid sequence represented by SEQ ID NO: 1, (b) a polypeptide having an amino acid sequence, which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID NO: 1, and having culture performance for pluripotent stem cells, or (c) a polypeptide having an amino acid sequence, which is formed by the deletion, substitution, or addition of one amino acid or several amino acids in SEQ ID NO: 1, and having culture performance for pluripotent stem cells.

    摘要翻译: 多能干细胞的培养方法包括通过将多肽施用于载体的细胞培养物表面获得多肽包被的培养物表面,并通过使用培养基将多能干细胞接种到多肽包被的培养物表面上来培养多能干细胞 其中抗坏血酸衍生物的含量等于或大于1.5mmol / L(mM),其中多肽是(a)具有SEQ ID NO:1所示氨基酸序列的多肽,(b)a 具有氨基酸序列的多肽,其与SEQ ID NO:1所示的氨基酸序列具有等于或高于80%的同一性,并具有多能干细胞的培养性能,或(c)具有氨基酸的多肽 序列,其通过SEQ ID NO:1中的一个氨基酸或几个氨基酸的缺失,取代或添加而形成,并具有多能干细胞的培养性能。

    Cell culture media and methods
    87.
    发明授权
    Cell culture media and methods 有权
    细胞培养基和方法

    公开(公告)号:US09410118B2

    公开(公告)日:2016-08-09

    申请号:US14367413

    申请日:2012-12-21

    IPC分类号: B29C67/24 B29L9/00 C12N5/00

    摘要: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

    摘要翻译: 描述了制备培养基,饲料和补品的组合物和方法。 这样的方法和介质可以显示不稳定组分的增加的稳定性,并且可以使用例如微悬浮和/或封装技术,螯合,以及任选地,将不稳定化合物与抗氧化剂包衣和/或混合。 组合物可以承受热和/或照射治疗并且具有减少的病毒数量。 这些技术可能导致产品具有延长的保质期,将其内部组分延长释放到培养物中,或可以无菌地添加到生物反应器中使用最小体积的产品中。 组合物和方法可以优化生物生产工作流程并提高效率。

    Mesenchymal stem cells produced from human pluripotent stem cells
    89.
    发明授权
    Mesenchymal stem cells produced from human pluripotent stem cells 有权
    由人多能干细胞产生的间充质干细胞

    公开(公告)号:US09388384B2

    公开(公告)日:2016-07-12

    申请号:US13504881

    申请日:2009-10-28

    IPC分类号: C12N5/00 C12N5/0775 A61K35/48

    摘要: Provided is a method for producing mesenchymal stem cells from human pluripotent stem cells, the method including: a) forming embryonic bodies from human pluripotent stem cells; b) attaching the embryonic bodies to a culture dish to induce natural differentiation of the embryonic bodies into mesenchymal stem cells; and c) performing continuous proliferative culturing of the mesenchymal stem cells while still maintaining the identity of the mesenchymal stem cells. Also, provided is a standardized method for inducing differentiation of mesenchymal stem cells, which can be broadly applied to all human pluripotent stem cells regardless of a difference in the genetic background thereof. Ultimately, the present invention can continuously mass-produces the mesenchymal stem cells necessary for regenerative medicine and cell therapy by using human pluripotent stem cells, thereby realizing practical uses of cell therapy products, and further the present invention is expected to highly contribute to treatments of incurable diseases, such as cardiovascular diseases and neurological disorders.

    摘要翻译: 提供一种从人多能干细胞产生间充质干细胞的方法,所述方法包括:a)从人多能干细胞形成胚体; b)将胚胎体附着到培养皿中以诱导胚胎体自然分化成间充质干细胞; 和c)对间充质干细胞进行连续增殖培养,同时仍保持间充质干细胞的特性。 此外,提供了诱导间充质干细胞分化的标准化方法,其可以广泛地应用于所有人多能干细胞,而与其遗传背景的差异无关。 最终,本发明可以通过使用人多能干细胞连续批量生产再生医学和细胞治疗所需的间充质干细胞,从而实现细胞治疗产品的实际应用,并且进一步预期本发明对 不治之症,如心血管疾病和神经系统疾病。