公开(公告)号：US20160033412A1

公开(公告)日：2016-02-04

申请号：US14813015

申请日：2015-07-29

Applicant: Ming TAN ,  Yun-Pei CHANG ,  Leyla SABET ,  Ashutosh SHASTRY ,  Christopher E. TODD ,  Reuven DUER

Inventor： Ming TAN ,  Yun-Pei CHANG ,  Leyla SABET ,  Ashutosh SHASTRY ,  Christopher E. TODD ,  Reuven DUER

IPC: G01N21/64 ,  G01N21/77

CPC classification number: G01N21/7703 ,  B01L3/502 ,  B01L2200/025 ,  B01L2200/0605 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/06 ,  G01N21/648 ,  G01N33/54373

Abstract: Optical readers and alignment tools for detecting the level of an analyte. Described herein are small, disposable partially-encapsulated sensing chips for detecting an analyte level from a fluid sample (e.g., a blood sample) having an edge of the integrated sensing chip exposed to directly expose a plurality of excitation and a collection waveguides, as well as optical readers and methods of operating them. A fluid sample maybe applied to a sensing surface of the sensing chip in the housing so that an analyte level can be optically detected. Also described are methods of sensing an analyte using these devices and systems including an optical detector.

Abstract translation: 用于检测分析物水平的光学读取器和对准工具。 这里描述的是用于从具有暴露于直接暴露多个激发和收集波导的集成感测芯片的边缘的流体样本（例如，血液样本）检测分析物水平的小的，一次性部分封装的感测芯片，以及 作为光学读取器和操作它们的方法。 流体样品可以施加到壳体中感测芯片的感测表面，使得可以光学地检测分析物水平。 还描述了使用包括光学检测器的这些装置和系统来感测分析物的方法。

公开(公告)号：US09249443B2

公开(公告)日：2016-02-02

申请号：US12867108

申请日：2008-11-20

Applicant: Aaron R. Wheeler ,  Irena Barbulovic-Nad

Inventor： Aaron R. Wheeler ,  Irena Barbulovic-Nad

IPC: C12Q1/02 ,  C12N5/02 ,  B01F13/00 ,  B01L3/00 ,  C12M3/06 ,  C12M1/12 ,  C12M1/26 ,  C12M1/34 ,  G01N33/543

CPC classification number: C12Q1/02 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/0688 ,  C12M23/16 ,  C12M25/01 ,  C12M25/08 ,  C12M33/00 ,  C12M41/00 ,  G01N33/54386

Abstract: Devices and methods for implementing cell-based assays and long-term cell culture. The device and method are based on digital microfluidics (DMF) which is used to actuate nanoliter droplets of reagents and cells on a planar array of electrodes. DMF method is suitable for assaying and culturing both cells in suspension and cells grown on surface (adherent cells). This method is advantageous for cell culture and assays due to the automated manipulation of multiple reagents in addition to reduced reagent use and analysis time. No adverse effects of actuation by DMF were observed in assays for cell viability, proliferation, and biochemistry. These results suggest that DMF has great potential as a simple yet versatile analytical tool for implementing cell-based assays and cell culture on the microscale.

Abstract translation: 用于实施基于细胞的测定和长期细胞培养的装置和方法。 该装置和方法基于数​​字微流体（DMF），其用于在平面电极阵列上致动纳升滴液滴的试剂和细胞。 DMF法适用于悬浮细胞和表面生长细胞（贴壁细胞）的测定和培养。 除了减少试剂使用和分析时间外，由于多种试剂的自动操作，该方法对于细胞培养和测定是有利的。 在细胞活力，增殖和生物化学的测定中没有观察到由DMF引起的不利影响。 这些结果表明，DMF具有巨大的潜力，作为一种简单而通用的分析工具，用于在微量级上实施基于细胞的测定和细胞培养。

公开(公告)号：US09248448B2

公开(公告)日：2016-02-02

申请号：US14258770

申请日：2014-04-22

Applicant: William Marsh Rice University

Inventor： John McDevitt ,  Nicolaos Christodoulidies ,  Pierre N. Floriano ,  Tim Abram

IPC: A61J1/06 ,  B01L3/00 ,  G01N33/574

CPC classification number: B01L3/5027 ,  B01L3/5025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0683 ,  G01N33/57407

Abstract: A bionanochip cartridge for analysis of multiple samples or analytes is provided herein, and the cartridge is dimensioned to take advantage of existing robotic microtiter plate handling equipment. Fluidics are specially designed to provide a small footprint and to prevent cross contamination.

Abstract translation: 本文提供了一种用于分析多种样品或分析物的生物纳米芯片盒，其尺寸设计为利用现有的机器人微量滴定板处理设备。 流体特别设计用于提供小的占地面积并防止交叉污染。

公开(公告)号：US20160023206A1

公开(公告)日：2016-01-28

申请号：US14341074

申请日：2014-07-25

Applicant: General Electric Company

Inventor： Ralf Lenigk ,  Erin Jean Finehout ,  Xuefeng Wang

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/5023 ,  A61B5/150343 ,  A61B5/150358 ,  B01L3/0258 ,  B01L3/0262 ,  B01L3/502715 ,  B01L3/561 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/16 ,  B01L2300/069 ,  B01L2300/0838 ,  B01L2300/0887 ,  B01L2300/161 ,  B01L2400/0406 ,  C12Q1/6806 ,  G01N1/14 ,  G01N2001/149

Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface and an outlet for driving out the sample fluid. The device further comprises an interface assembly comprising: a third layer, a fourth layer, a fifth layer, and a flow path. The interface assembly is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer of the interface assembly and the outlet is in contact with the flow path of the interface assembly for driving out the sample fluid from the integrated device.

Abstract translation: 提供了一种用于样品收集和转移的集成设备。 集成装置包括设置在第一层和第二层之间的毛细通道，其中第一层包括亲水层，该亲水层包括用于将样品流体接收到毛细通道的流体入口，其中毛细通道包括内表面和外表面 表面和用于排出样品流体的出口。 该装置还包括界面组件，其包括：第三层，第四层，第五层和流路。 接口组件设置在毛细管的外表面上，在相对于出口的确定位置处，使得毛细管与接口组件的第三层接触并且出口与界面的流动路径接触 用于从集成装置排出样品流体的组件。

公开(公告)号：US09216413B2

公开(公告)日：2015-12-22

申请号：US13381148

申请日：2010-06-08

Applicant: Gert Blankenstein ,  Christian Schoen

Inventor： Gert Blankenstein ,  Christian Schoen

IPC: B01D63/00 ,  B01D17/00 ,  B01D29/00 ,  B01D35/00 ,  G01N33/50 ,  G01N33/49 ,  B01L3/00 ,  E03B7/07 ,  G01N33/543 ,  G01N33/558

CPC classification number: B01L3/502738 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2200/0684 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/165 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L2400/088 ,  G01N33/491 ,  Y10T137/0318 ,  Y10T137/794

Abstract: The invention relates to a microfluidic apparatus for producing a volume flow that is uniform over time in a metering process. In various solution processes or reaction processes it is essential to have a specified given volume flow or mass flow of fluid available to ensure reliable dissolution of the reagent or ensure that the reaction takes place. In microfluidic apparatus in which separation of particles from a fluid, particularly blood, is carried out through a membrane, the volume flow through the membrane decreases continuously. In order to achieve a uniform volume flow during metering, it is envisaged that first of all a reservoir is filled from a first channel, so that the contents of the reservoir can then be fed to the metering process by opening a fluid stop. The emptying of the reservoir takes place with a uniform volume flow of 0.05 microliters per second to 10 microliters per second.

Abstract translation: 本发明涉及用于在计量过程中产生随时间均匀的体积流的微流体装置。 在各种溶液方法或反应过程中，必须具有指定的给定体积流量或质量流量的流体，以确保试剂的可靠溶解或确保反应发生。 在通过膜进行颗粒与流体特别是血液分离的微流体装置中，通过膜的体积流量连续减小。 为了在计量期间实现均匀的体积流量，设想首先从第一通道填充储存器，以便随后通过打开流体阻塞将储存器的内容物送入计量过程。 储存器的排空以0.05微升/秒至10微升/秒的均匀体积流量进行。

公开(公告)号：US09213043B2

公开(公告)日：2015-12-15

申请号：US14401275

申请日：2013-05-15

Applicant: Wellstat Diagnostics, LLC

Inventor： Richard Alan Cook ,  Sang Cho ,  Charles Quentin Davis ,  Kevin E Dorsey ,  Jason Charles Harley ,  Jonathan Leland ,  Rober Krikor Matikyan ,  Sjef Otten ,  Jeffrey Howard Peterman ,  Brian B Thomas

IPC: G01N21/00 ,  G01N35/10 ,  G01N35/00 ,  G01N33/58 ,  G01N21/76 ,  F04B49/06 ,  F04B49/10 ,  G01N27/327 ,  G01N33/536 ,  G01N33/543 ,  B01L3/00

CPC classification number: G01N35/1079 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/105 ,  B01L2400/0487 ,  B01L2400/049 ,  F04B49/065 ,  F04B49/106 ,  G01N21/76 ,  G01N27/3273 ,  G01N33/536 ,  G01N33/543 ,  G01N33/58 ,  G01N33/582 ,  G01N35/00722 ,  G01N35/0098 ,  G01N2035/0091 ,  Y10T436/119163 ,  Y10T436/25 ,  Y10T436/2575

Abstract: In embodiments disclosed herein, a diagnostic system is provided having a cartridge comprising at least one needle; at least one reservoir; at least one fluidic seal; and at least one fluidic channel of a fluidic pathway, wherein the cartridge is configured to store at least one reagent and at least one waste material on the cartridge. The diagnostic system is provided also having a diagnostic instrument comprising the fluidic pathway; an electrochemiluminescence (ECL) detection system; and a pump, wherein the fluidic pathway begins and ends in the cartridge and has a substantially single direction of flow in a pathway fluidically connecting the diagnostic instrument and the cartridge.

Abstract translation: 在本文公开的实施例中，提供了具有包括至少一个针的药筒的诊断系统; 至少一个水库; 至少一个流体密封; 以及至少一个流体通道的流体通道，其中所述筒被构造成在所述筒上存储至少一种试剂和至少一种废料。 该诊断系统还具有包括流体通路的诊断仪器; 电化学发光（ECL）检测系统; 和泵，其中流体通道在药筒中开始和结束，并且在流体连接诊断​​仪器和药筒的通路中具有基本上单一的流动方向。

公开(公告)号：US20150321192A1

公开(公告)日：2015-11-12

申请号：US14803161

申请日：2015-07-20

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Beom Seok LEE

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/086 ,  Y10T436/2575

Abstract: A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers.

Abstract translation: 微流体结构，其中布置在不同位置的多个腔室并联连接，并且固定量的流体可以在不使用单独的驱动源的情况下有效地分配到该微流体结构中，以及具有该微流体结构的微流体装置。 微流体装置包括具有旋转中心并包括至少一个微流体结构的平台。 微流体结构包括被配置为容纳样品的样品供应室，在与平台的旋转中心不同的距离处在平台的圆周方向上布置的多个第一室和多个虹吸通道，每个虹吸管 通道连接到相应的一个第一室。

公开(公告)号：US09182322B2

公开(公告)日：2015-11-10

申请号：US14091344

申请日：2013-11-27

Applicant: Fluidigm Corporation

Inventor： Tim Woudenberg ,  Jing Wang ,  Hou-Pu Chou

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N1/28 ,  H04W76/06 ,  H04W4/00

CPC classification number: B01L3/502715 ,  B01F13/0059 ,  B01F13/0094 ,  B01F13/1013 ,  B01F13/1022 ,  B01J2219/00317 ,  B01J2219/00479 ,  B01J2219/00585 ,  B01L3/5025 ,  B01L3/502723 ,  B01L3/502738 ,  B01L3/502746 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  B01L2400/082 ,  B33Y80/00 ,  G01N1/28 ,  H04W4/70 ,  H04W76/30

Abstract: Microfluidic devices are described that include a rigid base layer, and an elastomeric layer on the base layer. The elastomeric layer may include at least part of a fluid channel for transporting a liquid reagent, and a vent channel that accepts gas diffusing through the elastomeric layer from the flow channel and vents it out of the elastomeric layer. The devices may also include a mixing chamber fluidly connected to the fluid channel, and a control channel overlapping with a deflectable membrane that defines a portion of the flow channel, where the control channel may be operable to change a rate at which the liquid reagent flows through the fluid channel. The devices may further include a rigid plastic layer on the elastomeric layer.

Abstract translation: 描述了包括刚性基层和基层上的弹性体层的微流体装置。 弹性体层可以包括用于输送液体试剂的流体通道的至少一部分，以及排气通道，其接纳从流动通道穿过弹性体层扩散的气体并将其从弹性体层排出。 装置还可以包括流体连接到流体通道的混合室，以及与限定流动通道的一部分的可偏转膜重叠的控制通道，其中控制通道可操作以改变液体试剂流动的速率 通过流体通道。 所述装置还可包括弹性体层上的刚性塑料层。

公开(公告)号：US20150299768A1

公开(公告)日：2015-10-22

申请号：US14434069

申请日：2013-10-08

Applicant: JN MEDSYS PTE LTD

Inventor： Kian Kok Johnson NG ,  Koon Kiat TEU

IPC: C12Q1/68 ,  B01L3/00 ,  G01N1/14

CPC classification number: C12Q1/6806 ,  B01L3/50857 ,  B01L2200/0605 ,  B01L2300/0893 ,  B01L2400/0406 ,  B01L2400/0409 ,  C12Q1/686 ,  G01N1/14 ,  G01N2001/149 ,  G01N2035/1039 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: A device comprising: a capillary array of bundled micro-capillary wells; said bundle arranged into a close packed arrangement; a first end of said array forming a sample receiving surface; wherein said device is arranged to draw said sample into said capillary array through capillary action, so as to divide the sample into a plurality of sub-reactions in said wells.

Abstract translation: 一种装置，包括：捆绑的微毛细管孔的毛细管阵列; 所述捆包布置成紧密包装; 所述阵列的第一端形成样品接收表面; 其中所述装置被布置成通过毛细管作用将所述样品吸入所述毛细管阵列中，以便将样品分成所述孔中的多个亚反应。

公开(公告)号：US20150293097A1

公开(公告)日：2015-10-15

申请号：US14434273

申请日：2013-10-07

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Richard Douglas Godec ,  Paul Charles Melanson ,  Matthew Kaddeland Stonesmith ,  Hong Xu ,  Yan Huang

IPC: G01N33/579 ,  B01L3/00

CPC classification number: G01N33/56911 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/5085 ,  B01L2200/0605 ,  B01L2200/0684 ,  B01L2300/021 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/16 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0622 ,  B01L2400/0688 ,  B01L2400/0694 ,  G01N21/17 ,  G01N33/579 ,  G01N2400/10 ,  G01N2400/50 ,  Y10T436/2575

Abstract: A test substrate for detecting a LAL-reactive substance, wherein at least a portion of said test substrate has been pre-loaded with at least one LAL reagent and/or at least one LAL-reactive standard. Methods of use of the test substrate are disclosed. Methods of depositing test reagents on a test substrate are also disclosed.

Abstract translation: 一种用于检测LAL反应物质的测试基底，其中所述测试基底的至少一部分已经预加载了至少一种LAL试剂和/或至少一种LAL反应性标准物。 公开了使用测试基板的方法。 还公开了将测试试剂沉积在测试基底上的方法。