公开(公告)号：US20150247845A1

公开(公告)日：2015-09-03

申请号：US14409703

申请日：2013-06-21

申请人： SCANDINAVIAN MICRO BIODEVICES

发明人： Martin Bak Heller ,  Bent Overby ,  Niels Kristian Bau-Madsen

IPC分类号： G01N33/543 ,  B01L3/00 ,  G01N33/12 ,  G01N21/64 ,  G01N33/58 ,  G01N33/04

CPC分类号： G01N33/54333 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2300/123 ,  B01L2300/18 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/049 ,  B82Y15/00 ,  G01N21/6428 ,  G01N21/6489 ,  G01N33/04 ,  G01N33/12 ,  G01N33/5005 ,  G01N33/53 ,  G01N33/54326 ,  G01N33/54386 ,  G01N33/569 ,  G01N33/582 ,  G01N33/587 ,  G01N33/588 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6484 ,  G01N2033/0095

摘要： A method and a system for quantitative or qualitative determination of a target component in a liquid sample includes i) providing a plurality of magnetic particles including one or more capture sites for the target component on their respective surfaces; ii) providing a plurality of fluorophores configured to bind to the capture sites of the magnetic particles; iii) bringing the liquid sample into contact with the fluorophores and the magnetic particles in a flow channel of a micro fluidic device including a transparent window; and iv) at least temporally immobilizing the magnetic particles adjacent to the transparent window using a magnet, emitting exciting electromagnetic beam towards the immobilized magnetic particles, reading signals emitted from fluorophores captured by the immobilized magnetic particles and performing a quantitative or qualitative determination of the target component based on the read signal.

摘要翻译： 用于定量或定性地测定液体样品中的目标组分的方法和系统包括：i）在其各自的表面上提供多个磁性颗粒，包括用于靶组分的一个或多个捕获位点; ii）提供配置成结合到磁性颗粒的捕获位点的多个荧光团; iii）使液体样品与包括透明窗口的微流体装置的流动通道中的荧光团和磁性颗粒接触; 以及iv）使用磁体至少在时间上固定与透明窗口相邻的磁性颗粒，向固定的磁性颗粒发射激发电磁波，读取由固定的磁性颗粒捕获的荧光团发射的信号，并对目标进行定量或定性测定 基于读取信号的分量。

公开(公告)号：US20150241401A1

公开(公告)日：2015-08-27

申请号：US14625308

申请日：2015-02-18

申请人： AEMTEK, INC.

发明人： FLORENCE WU ,  YONGQING HUANG ,  TERRANCE ARTHUR ,  TOMMY WHEELER

IPC分类号： G01N33/12 ,  G01N1/04 ,  G01N35/02

摘要： Systems and methods for sampling within a conveyance system are provided herein, such systems and methods being particularly useful in batch sampling of food products for a targeted biological agent. In one aspect, the sampling system includes a conveyance system and one or more sampling devices positioned along a conveyance path such that at least one portion of the batch contacts a sampling medium of the one or more sampling devices. In another aspect, sampling devices are provided that allow a sampling member to be secured in a sampling position for batch testing and readily removed after sampling and tested. The systems, devices and methods herein provide improved sampling coverage of the entire batch and reduce waste and inefficiency as compared to conventional batch sampling methods.

摘要翻译： 本文提供了在输送系统内采样的系统和方法，这样的系统和方法特别适用于对目标生物制剂的食品的批量采样。 在一个方面，采样系统包括输送系统和沿着输送路径定位的一个或多个采样装置，使得批料的至少一部分接触一个或多个采样装置的采样介质。 在另一方面，提供了采样装置，其允许将取样构件固定在采样位置进行批量测试，并在采样和测试后容易地移除。 与常规批量采样方法相比，本文的系统，装置和方法提供整个批次的改进的采样覆盖并且减少浪费和低效率。

公开(公告)号：US09097672B2

公开(公告)日：2015-08-04

申请号：US13704719

申请日：2011-06-17

申请人： R. Stephen Brown ,  Eric Marcotte ,  Doug Wilton ,  Peter Gallant ,  David Dolphin ,  Lee Underwood

发明人： R. Stephen Brown ,  Eric Marcotte ,  Doug Wilton ,  Peter Gallant ,  David Dolphin ,  Lee Underwood

IPC分类号： G01N21/64 ,  G01N33/12 ,  G01N33/18 ,  B01L3/00

CPC分类号： G01N21/6486 ,  B01L3/50825 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/12 ,  G01N33/1826 ,  G01N2021/6417 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2201/062

摘要： A system for detecting presence of an organism having an enzyme in a sample, comprising: a cartridge for containing the sample and a substrate such that the enzyme can react with the substrate to produce a biological molecule; a partitioning element mounted in a recess in a base of the cartridge, the partitioning element allowing partitioning of the biological molecule thereinto; a light source for irradiating the biological molecule partitioned into the partitioning element; and, a detector for detecting fluorescence of the biological molecule partitioned into the partitioning element, the detected fluorescence being indicative of presence of the organism in the sample; wherein the light source is in a raised cartridge mount of the system that mates with the recess in the base of the cartridge.

摘要翻译： 一种用于检测样品中具有酶的生物体的存在的系统，包括：用于容纳样品和底物的筒，使得酶可以与底物反应以产生生物分子; 安装在所述盒的基部中的凹部中的分隔元件，所述分隔元件允许生物分子在其中分配; 用于照射被划分成分隔元件的生物分子的光源; 以及检测器，用于检测划分成分隔元件的生物分子的荧光，检测到的荧光指示样品中生物体的存在; 其中所述光源位于所述系统的凸起的盒安装件中，以与所述盒的所述底座中的凹部配合。

公开(公告)号：US09091632B2

公开(公告)日：2015-07-28

申请号：US14038900

申请日：2013-09-27

申请人： CEM Corp.

发明人： Michael J Collins, Sr. ,  Joseph J Lambert ,  Timothy A Zawatsky ,  David L Herman

IPC分类号： G01N33/00 ,  G01N1/00 ,  G01N21/17 ,  G01N33/68 ,  G01N21/25 ,  G01N1/28 ,  G01N35/10 ,  G01N33/12 ,  G01N1/40

CPC分类号： G01N33/6839 ,  G01N1/286 ,  G01N21/17 ,  G01N21/251 ,  G01N33/12 ,  G01N33/6803 ,  G01N35/1095 ,  G01N2001/4088 ,  Y10T436/10 ,  Y10T436/105831 ,  Y10T436/25

摘要： A dye binding method for protein analysis is disclosed. The method includes the steps of preparing an initial reference dye solution of unknown concentration from an initial reference dye concentrate and creating an electronic signal based upon the absorbance of the initial reference dye solution. Thereafter, an electronic signal is created based upon the absorbance of a dye filtrate solution prepared from the initial reference dye solution and an initial protein sample. The absorbance signals from the reference dye solution and the dye filtrate solution are sent to a processor that compares the respective absorbances and calculates the protein content of the protein sample based upon the difference between the absorbances. An electronic signal is created based upon the absorbance of a successive dye filtrate solution prepared from the reference dye solution and a successive protein sample, and the absorbance signal from the successive sample dye filtrate solution is sent to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate solution.

摘要翻译： 公开了用于蛋白质分析的染料结合方法。 该方法包括以下步骤：从初始参考染料浓缩物制备未知浓度的初始参考染料溶液，并基于初始参考染料溶液的吸光度产生电子信号。 此后，基于由初始参考染料溶液和初始蛋白质样品制备的染料滤液的吸光度，产生电子信号。 将来自参考染料溶液和染料滤液的吸光度信号送至处理器，该处理器根据吸光度之差来比较各吸光度，并计算蛋白质样品的蛋白质含量。 基于由参考染料溶液和连续蛋白质样品制备的连续染料滤液的吸光度产生电子信号，并将来自连续样品染料滤液的溶液的吸光度信号发送到处理器，以计算蛋白质含量 基于初始参考染料溶液的吸光度与连续的染料滤液的吸光度之间的差异的连续样品。

公开(公告)号：US08890089B2

公开(公告)日：2014-11-18

申请号：US13255288

申请日：2010-03-09

申请人： Michael Lee ,  Michael Theodorou ,  Helen Ougham ,  Howard Thomas

发明人： Michael Lee ,  Michael Theodorou ,  Helen Ougham ,  Howard Thomas

IPC分类号： G01N21/64 ,  G01N33/12 ,  C09B61/00

CPC分类号： C09B61/00 ,  G01N33/12

摘要： Described are methods for analyzing an animal carcass, meat obtained therefrom, or product produced by or obtained from an animal for the presence or absence of faecal matter. The methods comprise analyzing an animal carcass, meat obtained therefrom, or product produced by or obtained from the animal for the presence or absence of a detectable marker, the presence of the detectable marker being indicative of the presence of faecal matter and the absence of the detectable marker being indicative of the absence of faecal matter, wherein the animal carcass, meat obtained therefrom, or product produced by or obtained from the animal has been obtained from an animal fed a composition comprising a supplement of the detectable marker and/or a precursor thereof. Also described are compositions for feeding to an animal and for use in the methods.

摘要翻译： 描述了用于分析动物屠体，从其获得的肉，或由动物产生或获得的用于粪便物质的存在或不存在的产品的方法。 所述方法包括分析动物屠体，从其获得的肉，或由动物产生或获自动物的产物，以检测标记的存在或不存在，可检测标记物是否存在指示粪便物质的存在和不存在 可检测标记指示不存在粪便物质，其中动物尸体，由其获得的肉或由动物产生或从动物获得的产品已经从喂食包含可检测标记物和/或前体的补充物的组合物的动物获得 其中。 还描述了用于喂养动物并用于该方法的组合物。

公开(公告)号：US08390271B2

公开(公告)日：2013-03-05

申请号：US12599948

申请日：2008-04-23

申请人： Michele Cardone ,  Giovanni Cosmai ,  Roberto Faranda ,  Antonino Giglio

发明人： Michele Cardone ,  Giovanni Cosmai ,  Roberto Faranda ,  Antonino Giglio

IPC分类号： G01N33/12

CPC分类号： B23Q3/154 ,  B25B11/002 ,  B66C1/04 ,  B66C15/065 ,  H01F7/206 ,  H01F2007/208

摘要： The present invention relates to magnetic anchorage equipment comprising a frame able to contain a plurality of polar units, each of the plurality of polar units having a ferromagnetic polar element which identifies an anchorage surface and a self-testing unit for checking the magnetic equipment. The magnetic anchorage equipment is characterized in that the self-testing unit for checking the magnetic equipment is at least partly integrated in the frame.

摘要翻译： 本发明涉及磁性锚固设备，其包括能够容纳多个极性单元的框架，多个极性单元中的每一个具有识别锚固表面的铁磁极性元件和用于检查磁性设备的自检单元。 磁性锚固设备的特征在于，用于检查磁性设备的自检单元至少部分地集成在框架中。

公开(公告)号：US08225645B2

公开(公告)日：2012-07-24

申请号：US12442026

申请日：2007-09-20

申请人： Jean-Francois Charles Meullenet

发明人： Jean-Francois Charles Meullenet

IPC分类号： G01N33/12 ,  G01N3/24

CPC分类号： A22B5/0064

摘要： An apparatus and method for predicting meat tenderness, particularly with respect to raw meat, is disclosed. The invention does not require the removal or destruction of any cuts of meat from the carcass to which the method is applied. The method allows for the identification of tender meat product that might not be identified as tender using conventional United States Department of Agriculture quality grading methods. The method includes the insertion of one or more blunt, flat-tipped blades into a meat sample, measuring a value such as stress, force, or energy upon insertion of the blade, and calculating a tenderness factor therefrom based on a tenderness threshold.

摘要翻译： 公开了一种用于预测肉嫩的装置和方法，特别是对于生肉。 本发明不需要从应用该方法的屠体中去除或破坏任何切肉。 该方法允许使用美国农业部的质量分级方法鉴定可能不被识别为嫩的嫩肉产品。 该方法包括将一个或多个钝的平头叶片插入肉样中，在插入叶片时测量诸如应力，力或能量的值，并根据压痛阈值计算其触痛因子。

公开(公告)号：US20120040845A1

公开(公告)日：2012-02-16

申请号：US13046032

申请日：2011-03-11

申请人： Mansour Samadpour

发明人： Mansour Samadpour

IPC分类号： C40B30/00 ,  C12Q1/04 ,  G01N33/12 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： A22B5/201 ,  A22B5/0064

摘要： The invention provides methods for certification of carcasses, and for detecting a contaminated carcass and preventing its movement into or across a production area. The inventive methods comprise obtaining, early in the production process (pre-fabrication), a test sample from at least one test location of at least one split-portion of each carcass, wherein the test samples are obtained prior to or during chilling of the respective split portions, before entry thereof in the production chain. Composite test samples are assayed for pathogens or microbes, whereby certification is afforded, or whereby entry of the chilled split-carcass-Lot into the production area is precluded if the corresponding composite-Lot test sample is contaminated. Methods for remedial reconditioning of contaminated split-carcasses are provided, wherein essentially 100% of the carcasses are targeted to the production line. The inventive methods provide substantial public health benefit, and are efficient and economical to implement.

摘要翻译： 本发明提供了用于认证屠体的方法，以及用于检测被污染的胴体并防止其移动进入生产区域或穿过生产区域的方法。 本发明的方法包括在生产过程（预制造）的早期获得来自每个胎体的至少一个分裂部分的至少一个测试位置的测试样品，其中在冷却之前或之后获得测试样品 相应的分割部分，在其进入生产链之前。 测试复合试验样品中的病原体或微生物，从而获得认证，或者如果相应的复合批试样被污染，则排除冷冻的分裂胴体进入生产区域。 提供了污染分解胴体的补救性修复方法，其中基本上100％的屠体被靶向生产线。 本发明的方法提供了显着的公共卫生益处，并且是有效和经济的。

公开(公告)号：US20120028359A1

公开(公告)日：2012-02-02

申请号：US13263182

申请日：2010-04-06

申请人： In Ho Choi ,  Dong Mok Lee ,  Jong Soo Chang ,  Hyun Jeong Lee ,  Eun Ju Lee ,  Bajracharya Prati ,  Yu Mi Shin

发明人： In Ho Choi ,  Dong Mok Lee ,  Jong Soo Chang ,  Hyun Jeong Lee ,  Eun Ju Lee ,  Bajracharya Prati ,  Yu Mi Shin

IPC分类号： G01N33/12 ,  C07C57/03 ,  C07H21/02 ,  C07C53/00 ,  G01N33/92 ,  C07K16/18

CPC分类号： G01N33/92 ,  C12Q1/6883 ,  C12Q1/6888 ,  C12Q2600/124 ,  G01N33/743 ,  G01N2800/044

摘要： The present invention relates to a production process of a gender-specific serum and a biomarker using the serum. More specifically, the present invention uses a fatty acid that exhibits a specific expression pattern in a gender-specific serum as a biomarker not only for diagnosis of obesity or a disease related to obesity, but also for diagnosis of meat quality since the fatty acid promotes differentiation of muscle derived stem cells into adipose cells. In addition, the present invention can establish a research system studying the effects of steroid hormones on the cell culture by using sera separated from blood that is collected from individual mammal carcasses being disposed, and provide important clues for discovering a gene associated with the synthesis of steroid hormone and for developing treatments for human diseases. Further, the present invention may contribute to increased profits derived from producing high quality sera, a reduced cost with treatment for carcass wastes, and promotion of the eco-industry for reducing environmental hormones.

摘要翻译： 本发明涉及使用血清的性别特异性血清和生物标志物的制备方法。 更具体地，本发明使用在性别特异性血清中表现出特异性表达模式的脂肪酸作为生物标志物，不仅用于诊断肥胖症或与肥胖有关的疾病，而且还用于肉质量的诊断，因为脂肪酸促进 将肌肉干细胞分化为脂肪细胞。 此外，本发明可以建立研究系统，通过使用从被处置的各个哺乳动物尸体收集的血液分离的血清，研究类固醇激素对细胞培养物的作用，并提供发现与合成相关的基因的重要线索 类固醇激素和用于开发人类疾病的治疗。 此外，本发明可以有助于生产高质量血清的利润增加，屠体废物处理成本降低，促进生态工业减少环境激素。

公开(公告)号：US20100112149A1

公开(公告)日：2010-05-06

申请号：US12550692

申请日：2009-08-31

申请人： Eric A. Johnson ,  Marite Bradshaw ,  Kristin M. Marshall

发明人： Eric A. Johnson ,  Marite Bradshaw ,  Kristin M. Marshall

IPC分类号： G01N33/02 ,  G01N33/04 ,  G01N33/12 ,  G01N33/03 ,  C12N1/21

CPC分类号： C07K14/33 ,  C12N15/09

摘要： A mutant strain of the bacterium Clostridium botulinum having an inactivated botulinal neurotoxin gene is disclosed. The mutant strain contains an artificially created and inserted modified intron vector between nucleotides 580 and 581 of the sense strand of the gene. The mutant strain can be used in microbiological challenge testing of foods and food processing methods.

摘要翻译： 公开了具有失活的肉毒杆菌神经毒素基因的肉毒梭菌的突变菌株。 突变菌株在基因的有义链的核苷酸580和581之间含有人工产生和插入的修饰的内含子载体。 突变菌株可用于食品和食品加工方法的微生物攻毒检测。