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公开(公告)号：US08740994B2
公开(公告)日：2014-06-03
申请号：US14116757
申请日：2012-05-10
Applicant: Yoshihiko Hirose , Yukihide Sato
Inventor： Yoshihiko Hirose , Yukihide Sato
CPC classification number: A61K8/99 , A61K8/66 , A61Q5/10 , C12N9/001 , C12N9/0057 , C12N9/0059 , C12N9/0061 , C12N9/0063 , C12N9/0071 , C12P13/00 , C12Y103/03005 , C12Y110/03001 , C12Y110/03002 , C12Y110/03003 , C12Y110/03004 , C12Y114/18001 , D06P1/32 , D06P3/14
Abstract: The object is to provide a process for dyeing that can provide an excellent dyeing effect while utilizing an oxidase, and a dyeing agent for use in the process. Provided is a dyeing agent comprising a modified enzyme obtained by adding positive charge by a chemical modification with an amine to an enzyme selected from the group consisting of an enzyme specified as EC 1.10.3.1, an enzyme specified as EC 1.10.3.2, an enzyme specified as EC 1.3.3.5, an enzyme specified as EC 1.10.3.4, an enzyme specified as EC 1.10.3.3 and an enzyme specified as EC 1.14.18.1. A process for dyeing using the dyeing agent is also provided.
Abstract translation: 本发明的目的是提供一种可以在使用氧化酶时提供优异的染色效果的染色方法和用于该方法的染色剂。 提供了一种染色剂，其包含通过化学修饰将正电荷与胺相加的酶所选择的酶所选择的酶，所述酶选自指定为EC1.10.3.1的酶，规定为EC1.10.3.2的酶，酶 规定为EC 1.3.3.5，规定为EC 1.10.3.4的酶，指定为EC 1.10.3.3的酶和指定为EC 1.14.18.1的酶。 还提供了使用染色剂进行染色的方法。
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公开(公告)号：US20120295324A1
公开(公告)日：2012-11-22
申请号：US13464681
申请日：2012-05-04
Applicant: Vladimir Elisashvili , Eva Kachlishvili , Tamas Torok
Inventor： Vladimir Elisashvili , Eva Kachlishvili , Tamas Torok
CPC classification number: C12N9/248 , C12N9/0061 , C12N9/0065 , C12N9/2437 , C12P21/00 , C12P39/00 , C12P2203/00 , C12Y110/03002 , C12Y111/01013
Abstract: Methods, compositions, and systems for overproducing ligninolytic enzymes from the basidiomycetous fungus are described herein. As described, the method can include incubating a fungal strain of Cerrena unicolor IB303 in a fermentation system having growth medium which includes lignocellulosic material and then cultivating the fungal strain in the fermentation system under conditions wherein the fungus expresses the ligninolytic enzymes. In some cases, the lignocellulosic material is mandarin peel, ethanol production residue, walnut pericarp, wheat bran, wheat straw, or banana peel.
Abstract translation: 本文描述了用于从basidiomycetous真菌过度产生木质素分解酶的方法，组合物和系统。 如上所述，该方法可以包括在具有包含木质纤维素材料的生长培养基的发酵系统中温育真菌菌株Cerrena unicolor IB303，然后在真菌表达木质素分解酶的条件下培养发酵系统中的真菌菌株。 在一些情况下，木质纤维素材料是普通话剥离，乙醇生产残留物，核桃果皮，麦麸，小麦秸秆或香蕉皮。
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公开(公告)号：US20110091934A1
公开(公告)日：2011-04-21
申请号：US12935644
申请日：2009-04-16
Applicant: Lars Henrik Oestergaard , Leonardo De Maria , Miguel Duarte Toscano
Inventor： Lars Henrik Oestergaard , Leonardo De Maria , Miguel Duarte Toscano
CPC classification number: D21H21/32 , C11D3/38636 , C11D3/38681 , C12N9/0061 , C12Y110/03002 , D21H11/00 , D21H17/005
Abstract: The present invention relates to variants of a parent laccase. The present invention also relates to polynucleotides encoding the variant laccases and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes.
Abstract translation: 本发明涉及母漆酶的变体。 本发明还涉及编码变体lacCases的多核苷酸以及包含多核苷酸的核酸构建体，载体和宿主细胞，以及使用变体酶的方法。
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公开(公告)号：US20110027381A1
公开(公告)日：2011-02-03
申请号：US12445635
申请日：2007-10-16
Applicant: Georg Gradl , Detlef Behrend , Klaus-Peter Schmitz , Katrin Sternberg , Kathleen Schmohl , Sven Kramer
Inventor： Georg Gradl , Detlef Behrend , Klaus-Peter Schmitz , Katrin Sternberg , Kathleen Schmohl , Sven Kramer
CPC classification number: A61K38/44 , A61K33/06 , A61K38/39 , A61K45/06 , A61L24/0084 , A61L27/46 , A61L2430/02 , C12Y110/03002 , C08L89/06 , A61K2300/00
Abstract: The present invention relates to a combination for the treatment of osteoporosis and/or the prophylaxis and treatment of bone fractures, said combination comprising collagen, an additional peptide, a calcium-containing substance and a wetting agent with a terminally functionalized oligolactone. The invention also relates to artificial bones and implants produced by the combination and to the use of said combination for fixing implants and treating osteoporosis and/or the prophylaxis and treatment of bone fractures. The invention further relates to a method for producing artificial bones and implants.
Abstract translation: 本发明涉及用于治疗骨质疏松症和/或预防和治疗骨折的组合，所述组合包括胶原，附加肽，含钙物质和具有末端官能化寡聚内酯的润湿剂。 本发明还涉及通过组合产生的人造骨和植入物，以及用于固定植入物和治疗骨质疏松症和/或骨折的预防和治疗的所述组合的使用。 本发明还涉及一种用于生产人造骨和植入物的方法。
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55.发明授权Laccases, nucleic acids encoding them and methods for making and using them 有权漆酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US07741089B2
公开(公告)日：2010-06-22
申请号：US10567536
申请日：2004-08-11
Applicant: Tim Hitchman , Dan E. Robertson , Masao Hiraiwa , Yoko Phillips , Kevin A. Gray
Inventor： Tim Hitchman , Dan E. Robertson , Masao Hiraiwa , Yoko Phillips , Kevin A. Gray
CPC classification number: C12H1/14 , A21D8/042 , A23C9/1213 , A23C9/152 , A23K20/189 , A23K40/20 , A23K40/25 , A23L27/29 , A23L29/06 , A24B15/20 , A61L2/186 , A61L2202/24 , C11D3/38636 , C12N9/0057 , C12N9/0061 , C12N15/1137 , C12N15/8243 , C12N15/8246 , C12N15/8255 , C12N2310/11 , C12N2310/14 , C12P7/26 , C12Y110/03002 , D21C5/005 , D21C9/1036 , G06F19/22
Abstract: The invention provides laccases, polynucleotides encoding these enzymes, the use of such polynucleotides and polypeptides. In one aspect, the invention relates to the enzymatic production of nootkatone by way of the conversion of valencene using proteins having a laccase activity, e.g., a novel laccase of the invention. In one aspect, the invention provides methods of depolymerizing lignin, e.g., in a pulp or paper manufacturing process, using a polypeptide of the invention. In another aspect, the invention provides methods for oxidizing products that can be mediators of laccase-catalyzed oxidation reactions, e.g., 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 1-hydroxybenzotriazole (HBT), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), dimethoxyphenol, and the like.
Abstract translation: 本发明提供漆酶，编码这些酶的多核苷酸，使用这种多核苷酸和多肽。 一方面，本发明涉及通过使用具有漆酶活性的蛋白质（例如本发明的新型漆酶）转化戊烯的酶促生产。 在一个方面，本发明提供使用本发明的多肽来解聚木质素的方法，例如在纸浆或造纸方法中。 另一方面，本发明提供氧化可能是漆酶催化氧化反应介质的产物的方法，例如2,2-二氮杂双（3-乙基苯并噻唑啉-6-磺酸酯）（ABTS），1-羟基苯并三唑（HBT）， 2,2,6,6-四甲基哌啶-1-基氧基（TEMPO），二甲氧基苯酚等。
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Patent Agency Ranking
公开(公告)号：US07622287B2
公开(公告)日：2009-11-24
申请号：US09732350
申请日：2000-12-07
Applicant: Allan Svendsen , Feng Xu
Inventor： Allan Svendsen , Feng Xu
CPC classification number: C12Y110/03002 , C11D3/38636 , C12N9/0061
Abstract: The present invention relates to a method of designing laccase mutants with increased oxidation potential and/or changed pH optimum and/or altered mediator pathway and/or altered O2/OH−-pathway, which method is based on the hitherto unknown three dimensional structure of laccases, wherein the parent laccase is a Myceliophthora thermophila, Polyporus pinsitus, or Coprinus cinereus laccase.
Abstract translation: 本发明涉及一种设计具有增加的氧化电位和/或改变的pH最佳和/或改变的介体途径和/或改变的O 2 / OH途径的漆酶突变体的方法，该方法基于迄今为止未知的三维结构 漆酶，其中母漆酶是嗜热丝孢杆菌，多孔菌或灰霉菌漆酶。
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57.发明申请FUSION PROTEINS BETWEEN PLANT CELL-WALL DEGRADING ENZYMES AND A SWOLLENIN, AND THEIR USES 审中-公开植物细胞壁降解酶和螺环素之间的融合蛋白及其用途公开(公告)号：US20090221039A1
公开(公告)日：2009-09-03
申请号：US12295870
申请日：2007-04-02
Applicant: Eric Record , Anthony Levasseur , Markku Soloheimo , David Navarro , Martina Andgerg-Blomster , Frederic Monot , Tiina Nakari-Setala , Marcel Asther
Inventor： Eric Record , Anthony Levasseur , Markku Soloheimo , David Navarro , Martina Andgerg-Blomster , Frederic Monot , Tiina Nakari-Setala , Marcel Asther
CPC classification number: C12N9/248 , A23L33/185 , C07K2319/01 , C12N9/0006 , C12N9/0061 , C12N9/0065 , C12N9/18 , C12N9/2405 , C12N9/2437 , C12N9/2477 , C12Y110/03002 , C12Y301/01073 , C12Y302/01091 , D21C5/005 , D21C9/10 , D21H17/005
Abstract: The invention relates to fusion proteins including at least a swollenin and at least a plant cell-wall degrading enzyme, the swollenin, and plant cell-wall degrading enzyme, being recombinant proteins corresponding to native proteins in fungi, or mutated forms thereof. The invention also relates to the use of fusion proteins as defined above, for carrying out processes of plant cell-wall degradation in the frame of the preparation, from plants or vegetal by-products, of compounds of interest located in plant cell-wall, or in the frame of the bleaching of pulp and paper, or for biofuel production, or food industries.
Abstract translation: 本发明涉及至少包含膨胀素和至少植物细胞壁降解酶，溶胀素和植物细胞壁降解酶的融合蛋白，其是对应于真菌中天然蛋白质的重组蛋白或其突变形式。 本发明还涉及如上定义的融合蛋白的用途，用于在植物或植物副产物的框架中进行植物细胞壁降解的方法，所述植物或植物副产物位于植物细胞壁中， 或在纸浆和纸张的漂白或生物燃料生产或食品工业的框架内。
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58.发明申请Laccases, nucleic acids encoding them and methods for making and using them 有权漆酶，编码它们的核酸和制备和使用它们的方法公开(公告)号：US20070105112A1
公开(公告)日：2007-05-10
申请号：US10567536
申请日：2004-08-11
Applicant: Tim Hitchman , Dan Robertson , Masao Hiraiwa , Yoko Phillips , Kevin Gray
Inventor： Tim Hitchman , Dan Robertson , Masao Hiraiwa , Yoko Phillips , Kevin Gray
CPC classification number: C12H1/14 , A21D8/042 , A23C9/1213 , A23C9/152 , A23K20/189 , A23K40/20 , A23K40/25 , A23L27/29 , A23L29/06 , A24B15/20 , A61L2/186 , A61L2202/24 , C11D3/38636 , C12N9/0057 , C12N9/0061 , C12N15/1137 , C12N15/8243 , C12N15/8246 , C12N15/8255 , C12N2310/11 , C12N2310/14 , C12P7/26 , C12Y110/03002 , D21C5/005 , D21C9/1036 , G06F19/22
Abstract: The invention provides laccases, polynucleotides encoding these enzymes, the use of such polynucleotides and polypeptides. In one aspect, the invention relates to the enzymatic production of nootkatone by way of the conversion of valencene using proteins having a laccase activity, e.g., a novel laccase of the invention. In one aspect, the invention provides methods of depolymerizing lignin, e.g., in a pulp or paper manufacturing process, using a polypeptide of the invention. In another aspect, the invention provides methods for oxidizing products that can be mediators of laccase-catalyzed oxidation reactions, e.g., 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 1-hydroxybenzotriazole (HBT), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), dimethoxyphenol, and the like.
Abstract translation: 本发明提供漆酶，编码这些酶的多核苷酸，使用这种多核苷酸和多肽。 一方面，本发明涉及通过使用具有漆酶活性的蛋白质（例如本发明的新型漆酶）转化戊烯的酶促生产。 在一个方面，本发明提供使用本发明的多肽来解聚木质素的方法，例如在纸浆或造纸方法中。 另一方面，本发明提供氧化可能是漆酶催化氧化反应介质的产物的方法，例如2,2-二氮杂双（3-乙基苯并噻唑啉-6-磺酸酯）（ABTS），1-羟基苯并三唑（HBT）， 2,2,6,6-四甲基哌啶-1-基氧基（TEMPO），二甲氧基苯酚等。
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公开(公告)号：US20060246545A1
公开(公告)日：2006-11-02
申请号：US11401696
申请日：2006-04-11
Applicant: Huaming Wang
Inventor： Huaming Wang
CPC classification number: C12N1/14 , C12N9/0061 , C12N9/48 , C12N9/62 , C12N9/88 , C12N15/52 , C12P21/00 , C12P21/02 , C12Y110/03002
Abstract: A recombinant filamentous fungal cell (e.g. Aspergillus) having one or more inactivated chromosomal genes is provided. The chromosomal genes in some embodiments correspond to derA, derB, htmA, mnn9, mnn10, ochA, dpp4, dpp5, pepAa, pepAb, pepAc, pepAd, pepF and combinations thereof. The recombinant fungal cells may include further inactivated chromosomal genes which correspond to pepA, pepB, pepC and pepD. The recombinant filamentous fungal cells may include a heterologous nucleic acid encoding a protein of interest. Also provided are methods of producing a protein of interest in said recombinant filamentous fungal cell.
Abstract translation: 提供了具有一个或多个失活的染色体基因的重组丝状真菌细胞（例如曲霉属）。 一些实施方案中的染色体基因对应于derA，derB，htmA，mnn9，mnn10，ochA，dpp4，dpp5，pepAa，pepAb，pepAc，pepAd，pepF及其组合。 重组真菌细胞可以包括对应于pepA，pepB，pepC和pepD的进一步灭活的染色体基因。 重组丝状真菌细胞可以包括编码目的蛋白质的异源核酸。 还提供了在所述重组丝状真菌细胞中产生感兴趣的蛋白质的方法。
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公开(公告)号：US20060183895A1
公开(公告)日：2006-08-17
申请号：US11397533
申请日：2006-04-03
Applicant: Leonard Bloksberg , Iikka Havukkala
Inventor： Leonard Bloksberg , Iikka Havukkala
IPC: C07H21/04
CPC classification number: C12N9/0004 , C12N9/0008 , C12N9/001 , C12N9/0059 , C12N9/0061 , C12N9/0065 , C12N9/0071 , C12N9/0083 , C12N9/1007 , C12N9/1011 , C12N9/1051 , C12N9/2422 , C12N9/2445 , C12N9/86 , C12N9/93 , C12N15/8255 , C12Y110/03001 , C12Y110/03002 , C12Y114/13011 , C12Y201/01006 , C12Y302/01001 , C12Y302/01021
Abstract: Novel isolated polynucleotides and polypeptides associated with the lignin biosynthetic pathway are provided, together with genetic constructs including such sequences. Methods for the modulation of lignin content, lignin structure and lignin composition in target organisms are also disclosed, the methods comprising incorporating one or more of the polynucleotides of the present invention into the genome of a target organism.
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