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公开(公告)号：US09593368B2
公开(公告)日：2017-03-14
申请号：US14321235
申请日：2014-07-01
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Christopher Michael Puleo , Patrick McCoy Spooner
CPC classification number: C12Q1/6848 , C12Q2531/119 , C12Q2531/125 , C12Q2565/518
Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; applying an aqueous buffer to the sample application zone of the substrate to washes away one or more inhibitors present on the sample application zone; and applying an isothermal nucleic acid amplification reaction mixture to the sample application zone to amplify the target nucleic acid to form a nucleic acid amplification product. The target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight.
Abstract translation: 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 将水性缓冲液施加到基材的样品施加区域以洗涤存在于样品施加区上的一种或多种抑制剂; 以及将等温核酸扩增反应混合物应用于样品施用区域以扩增靶核酸以形成核酸扩增产物。 具有第一分子量的靶核酸基本上固定在样品施用区，并且其中扩增产物具有第二分子量。
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公开(公告)号：US09587263B2
公开(公告)日：2017-03-07
申请号：US14225887
申请日：2014-03-26
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson , Paresh Lakhubhai Patel , Alison Myfanwy Wakefield , Stephen James Capper
CPC classification number: C12P19/34 , C12Q1/6844 , C12Q2527/107 , C12Q2527/125 , C12Q2527/137 , C12Q2531/119
Abstract: Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
Abstract translation: 本文提供了使用靶核酸模板的等温核酸扩增的方法和试剂盒; 包含具有链置换活性的DNA聚合酶，脱氧核糖核苷三磷酸（dNTP）混合物，具有3'端和5'末端的引物，分子拥塞试剂和用于扩增靶核酸模板的缓冲溶液的反应混合物 。 缓冲溶液维持反应混合物的低盐浓度，其中盐浓度导致底漆的熔融温度（Tm）低于反应温度至少10℃。 在等温条件下进行扩增。
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公开(公告)号：US20160153868A1
公开(公告)日：2016-06-02
申请号：US14557169
申请日：2014-12-01
Applicant: GENERAL ELECTRIC COMPANY
Inventor： John Richard Nelson , Wei Gao , Christopher Michael Puleo , Todd Frederick Miller , Christine Lynne Pitner , David Andrew Shoudy , Alex David Corwin
IPC: G01N1/10
CPC classification number: C12N15/1013 , B01L3/502 , B01L2200/025 , B01L2200/0689 , B01L2300/06 , B01L2300/0654 , B01L2300/0861 , C12Q1/6806 , G01N1/02 , G01N1/10 , G01N1/40 , G01N2001/028 , G01N2001/4038
Abstract: A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base; a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.
Abstract translation: 用于从感兴趣区域提取材料的系统包括流体输送基底，其包括形成在流体输送基底内的入口通道和出口通道; 固定到流体输送基座上的垫圈，其中垫圈包括暴露入口通道的开口端和出口通道的开口端的至少一个开口; 支撑件，其包括面向所述垫圈的样品支撑表面和相对表面; 以及对准构件，其在固定位置联接到所述相对表面，并且使得所述支撑件定位在所述流体输送基座和所述对准构件之间，其中所述对准构件或所述流体输送基座中的一个或两个被朝向彼此偏置 力（例如，磁体或弹簧力），并且其中流体输送基座可与支撑件分离并且构造成沿着样品支撑表面的平面移动以与对准构件对准。
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公开(公告)号：US09333463B2
公开(公告)日：2016-05-10
申请号：US13951929
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
IPC: G01N27/447 , B01D57/02
CPC classification number: B01D57/02 , G01N27/4473
Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.
Abstract translation: 提供了一种通过电洗脱从生物样品中洗脱生物分子的装置和系统。 用于从生物样品电洗脱生物分子的装置由构造成接收电解质和生物样品的壳体构成，至少两个电极包括可操作地耦合到壳体的导电氧化还原聚合物，以及生物分子不可渗透层，其设置在至少一个 电极。 生物分子不渗透层设置在至少一个电极上以防止生物分子到达电极。 提供了一种系统，其中系统包括样品采集端口，一个或多个包含缓冲液，溶剂，试剂或其组合的储存器，用于电洗脱的装置和控制器。
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35.发明申请公开(公告)号：US20160123926A1
公开(公告)日：2016-05-05
申请号：US14865478
申请日：2015-09-25
Applicant: General Electric Company
Inventor： John Richard Nelson , Christopher Michael Puleo , Erin Jean Finehout , Patrick McCoy Spooner , Kashan Ali Shaikh , Xiaohui Chen , Li Zhu
IPC: G01N27/447
CPC classification number: G01N27/44791 , C07H1/08 , C07H21/00 , C12N15/1017 , G01N27/44747
Abstract: A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
Abstract translation: 从生物材料中分离核酸的方法包括将生物材料应用于包含其中浸渍的一种或多种细胞裂解试剂的底物; 将液体施加到施加在基底上的生物材料; 从施加在基底上的生物材料中提取核酸; 并以基本上完整的形式收集所提取的核酸，其中所收集的核酸具有大于或等于20kb的分子量。
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Patent Agency Ranking
公开(公告)号：US20160053307A1
公开(公告)日：2016-02-25
申请号：US14933275
申请日：2015-11-05
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Ryan Charles Heller , Erik Leeming Kvam , John Richard Nelson
IPC: C12Q1/68
CPC classification number: C12Q1/6844 , C12Q1/6869 , C12Q2521/501 , C12Q2525/307 , C12Q2527/137 , C12Q2531/125
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.
Abstract translation: 本文提供了在单个反应容器中从线性DNA产生和扩增单链DNA环的方法，而没有任何介入的纯化步骤。 通过不依赖模板的单链DNA连接产生单链DNA环。 还提供了使用连接辅助的DNA扩增在单个管中的全基因组扩增线性染色体DNA。
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公开(公告)号：US09217167B2
公开(公告)日：2015-12-22
申请号：US13952040
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Ryan Charles Heller , John Richard Nelson , Erik Leeming Kvam
CPC classification number: C12P19/34 , C12Q1/6869 , C12Q2521/501 , C12Q2525/307 , C12Q2527/137
Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided.
Abstract translation: 本文提供了在单个反应容器中从线性DNA产生和扩增单链DNA环的方法，而没有任何介入的纯化步骤。 通过不依赖模板的单链DNA连接产生单链DNA环。 提供了从血液中提取的循环核酸的全基因组扩增。 还提供了用于执行所公开的方法的套件。
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公开(公告)号：US20150027891A1
公开(公告)日：2015-01-29
申请号：US13951881
申请日：2013-07-26
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nichole Lea Wood , Li Zhu , Craig Patrick Galligan
IPC: B01D57/02
CPC classification number: B01D57/02 , C12N15/101
Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
Abstract translation: 提供了通过电洗脱来从生物样品中洗脱生物分子如核酸的方法。 方法的实例包括各种步骤，例如将生物样品装载到包括壳体的装置中，至少两个可操作地耦合到壳体的导电氧化还原聚合物电极和设置在至少一个电极上的生物分子不可渗透层。 样品的负载之后是引发电连接以产生足以从生物样品中洗脱生物分子的电场强度; 并从生物样品中洗脱生物分子。
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公开(公告)号：US20140093878A1
公开(公告)日：2014-04-03
申请号：US13840062
申请日：2013-03-15
Applicant: General Electric Company
Inventor： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossman , Anuradha Sekher
CPC classification number: C12N9/22 , C07K1/00 , C07K14/00 , C12Q1/6806 , C12Q1/6844 , C12Q1/6858 , C12Y301/26 , C12Q2521/301 , C12Q2525/101 , C12Q2537/1376 , C12Q2527/101
Abstract: Provided herein are mutant endonuclease V enzymes that are capable of nicking an inosine-containing DNA sequence. Nucleic acid assays and agents that employ such mutant endonuclease V enzymes to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of a target DNA are also described.
Abstract translation: 本文提供了能够切割含有肌苷的DNA序列的突变体内切核酸酶V酶。 还描述了使用这种突变体内切核酸酶V酶将切口引入到包括一种或多种肌苷的靶DNA中并使用DNA聚合酶产生靶DNA扩增子的核酸测定法和试剂。
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40.发明申请公开(公告)号：US20190187142A1
公开(公告)日：2019-06-20
申请号：US15848301
申请日：2017-12-20
Applicant: General Electric Company
Inventor： Matthew Jeremiah Misner , Gregory Andrew Grossmann , Cathryn Ellen Olsen , John Richard Nelson , Brian Christopher Bales , David Roger Moore , Paul Michael Smigelski, JR.
IPC: G01N33/569 , G01N1/40 , G01N33/543 , B01L3/00 , B01D15/36
CPC classification number: G01N33/5695 , B01D15/363 , B01L3/5023 , B01L2200/0631 , B01L2200/10 , G01N1/4005 , G01N33/54366 , G01N2001/4011 , G01N2333/35 , G01N2405/06 , G01N2469/10
Abstract: A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.
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