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    Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture
    21.
    发明申请
    Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture 有权
    哺乳动物细胞系从细胞培养增加寿命和蛋白质产量

    公开(公告)号：US20120088299A1

    公开(公告)日：2012-04-12

    申请号：US13287395

    申请日：2011-11-02

    申请人： David M. Goldenberg ,  Zhengxing Qu ,  Chien-Hsing Chang ,  Edmund A. Rossi ,  Jeng-Dar Yang ,  Diane Nordstrom

    发明人： David M. Goldenberg ,  Zhengxing Qu ,  Chien-Hsing Chang ,  Edmund A. Rossi ,  Jeng-Dar Yang ,  Diane Nordstrom

    IPC分类号： C12N5/10

    CPC分类号： C07K16/2896 ,  A61K2039/505 ,  C07K14/005 ,  C07K14/4747 ,  C07K16/00 ,  C07K16/18 ,  C07K16/2803 ,  C07K16/3007 ,  C07K2317/24 ,  C07K2317/31 ,  C12N5/0694 ,  C12N15/85 ,  C12N15/86 ,  C12N2500/90 ,  C12N2501/48 ,  C12N2510/02 ,  C12N2710/20022 ,  C12N2740/10043 ,  C12P21/02

    摘要： Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hamiones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transformation under serum-free conditions. The method preferably involves eukaryotic cells, more preferably mammalian cells.

    摘要翻译： 公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白质，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，hamiones和疫苗。 用凋亡抑制基因或载体如三重突变体Bcl-2基因转染的细胞可以在培养物中更长时间地存活，导致蛋白质生物合成的状态和产量的延长。 这样的转染细胞表现出最大的细胞密度，其等于或超过由亲本细胞系实现的最大密度。 转染的细胞也可以预先适应于无血清培养基中生长，大大减少了在无血清培养基中获得蛋白质产生所需的时间。 在某些方法中，预先调节的细胞可以在无血清条件下转化后用于蛋白质生产。 该方法优选涉及真核细胞，更优选哺乳动物细胞。

    Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture
    24.
    发明申请
    Mammalian Cell Lines for Increasing Longevity and Protein Yield from a Cell Culture 有权
    哺乳动物细胞系从细胞培养增加寿命和蛋白质产量

    公开(公告)号：US20100311162A1

    公开(公告)日：2010-12-09

    申请号：US12819533

    申请日：2010-06-21

    申请人： David M. Goldenberg ,  Zhengxing Qu ,  Chien-Hsing Chang ,  Edmund A. Rossi ,  Jeng-Dar Yang ,  Diane Nordstrom

    发明人： David M. Goldenberg ,  Zhengxing Qu ,  Chien-Hsing Chang ,  Edmund A. Rossi ,  Jeng-Dar Yang ,  Diane Nordstrom

    IPC分类号： C12N5/10

    CPC分类号： C07K16/2896 ,  A61K2039/505 ,  C07K14/005 ,  C07K14/4747 ,  C07K16/00 ,  C07K16/18 ,  C07K16/2803 ,  C07K16/3007 ,  C07K2317/24 ,  C07K2317/31 ,  C12N5/0694 ,  C12N15/85 ,  C12N15/86 ,  C12N2500/90 ,  C12N2501/48 ,  C12N2510/02 ,  C12N2710/20022 ,  C12N2740/10043 ,  C12P21/02

    摘要： Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transformation under serum-free conditions. The method preferably involves eukaryotic cells, more preferably mammalian cells.

    摘要翻译： 公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，激素和疫苗。 用凋亡抑制基因或载体如三重突变体Bcl-2基因转染的细胞可以在培养物中更长时间地存活，导致蛋白质生物合成的状态和产量的延长。 这样的转染细胞表现出最大的细胞密度，其等于或超过由亲本细胞系实现的最大密度。 转染的细胞也可以预先适应于无血清培养基中生长，大大减少了在无血清培养基中获得蛋白质产生所需的时间。 在某些方法中，预先调节的细胞可以在无血清条件下转化后用于蛋白质生产。 该方法优选涉及真核细胞，更优选哺乳动物细胞。

    Method for recloning Chinese hamster ovary (CHO) cells
    26.
    发明授权
    Method for recloning Chinese hamster ovary (CHO) cells 有权
    重建中国仓鼠卵巢（CHO）细胞的方法

    公开(公告)号：US07833787B2

    公开(公告)日：2010-11-16

    申请号：US10922577

    申请日：2004-08-19

    申请人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

    发明人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

    IPC分类号： C12N5/07 ,  C12N5/10 ,  C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/0694 ,  C12N2500/90 ,  C12N2502/00 ,  C12N2502/30 ,  C12N2510/02

    摘要： A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

    摘要翻译： 用于选择克隆和重新克隆哺乳动物细胞的新方法，其对于生产生物药物，优选仓鼠或小鼠骨髓瘤细胞具有重要性，具有高度的自动化和生产能力。 本发明涉及沉积和复制所述细胞的单细胞克隆的方法。 本发明还涉及使用通过单细胞沉积获得和复制的细胞以及允许单细胞复制的组合物制备蛋白质的方法。

    Methods for protein expression in mammalian cells in serum-free medium
    27.
    发明授权
    Methods for protein expression in mammalian cells in serum-free medium 有权
    在无血清培养基中哺乳动物细胞中蛋白质表达的方法

    公开(公告)号：US07608425B2

    公开(公告)日：2009-10-27

    申请号：US11877728

    申请日：2007-10-24

    申请人： David M. Goldenberg ,  Chien-Hsing Chang ,  Edward A. Rossi ,  Diane Nordstrom

    发明人： David M. Goldenberg ,  Chien-Hsing Chang ,  Edward A. Rossi ,  Diane Nordstrom

    IPC分类号： C12P21/06 ,  C12N5/00 ,  C12N15/00

    CPC分类号： C07K14/4747 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/18 ,  C07K16/2803 ,  C07K16/3007 ,  C07K2317/24 ,  C07K2317/31 ,  C12N5/0694 ,  C12N2500/90 ,  C12N2501/48 ,  C12N2510/02 ,  C12N2740/16043 ,  C12P21/02

    摘要： Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transfection under serum-free conditions. In preferred embodiments, the cells of use are SpESF or SpESF-X cells.

    摘要翻译： 公开了用于增加细胞培养物的寿命并允许增加蛋白质生产的组合物和方法，优选重组蛋白，例如抗体，肽，酶，生长因子，白细胞介素，干扰素，激素和疫苗。 用凋亡抑制基因或载体如三重突变体Bcl-2基因转染的细胞可以在培养物中更长时间地存活，导致蛋白质生物合成的状态和产量的延长。 这样的转染细胞表现出最大的细胞密度，其等于或超过由亲本细胞系实现的最大密度。 转染的细胞也可以预先适应于无血清培养基中生长，大大减少了在无血清培养基中获得蛋白质产生所需的时间。 在某些方法中，预先修饰的细胞可用于在无血清条件下转染后的蛋白质生产。 在优选的实施方案中，使用的细胞是SpESF或SpESF-X细胞。

    Antigen presenting cells, method for their preparation and their use for cancer vaccines
    29.
    发明申请
    Antigen presenting cells, method for their preparation and their use for cancer vaccines 审中-公开
    抗原呈递细胞，其制备方法及其在癌症疫苗中的应用

    公开(公告)号：US20050002920A1

    公开(公告)日：2005-01-06

    申请号：US10898187

    申请日：2004-07-26

    申请人： Rita De Santis

    发明人： Rita De Santis

    IPC分类号： A61K35/12 ,  A61K35/48 ,  A61K39/00 ,  A61K48/00 ,  A61P35/00 ,  C07K14/705 ,  C07K14/82 ,  C12N5/09 ,  C12N5/10 ,  C12N15/85 ,  C12Q1/48 ,  C12N5/08

    CPC分类号： C12N5/0694 ,  A61K39/0011 ,  A61K2039/5152 ,  A61K2039/5154 ,  C12N2501/06 ,  C12N2501/23 ,  C12N2501/52 ,  C12N2501/59 ,  C12N2502/99 ,  C12N2506/11 ,  C12N2510/04

    摘要： The present invention discloses a method of generation of antigen presenting cells, comprising: a. collecting said cells from a subject, b. activating said collected cells; c. culturing and optionally expanding ex vivo said activated cells; d. treating said cultured and optionally expanded cells with DNA hypomethylating agents so that said cells concomitantly express multiple tumor associated antigens. The cells obtainable according to the method of the present invention, as well as the cellular components thereof whether alone or in combination with said cells, are useful for prevention and treatment of malignancies of different histotype that constitutively express one or more of the multiple tumor associated antigens that are expressed in said cells. Conveniently, said cells and/or cellular components are in the form of a vaccine. Said vaccines are advantageous over the prior art in that as they concomitantly express multiple/all methylation-regulated tumor associated antigens.

    摘要翻译： 本发明公开了一种产生抗原呈递细胞的方法，包括：a。 从受试者收集细胞，b。 激活所述收集的细胞; C。 培养和任选地扩展离体所述活化细胞; d。 用DNA低甲基化剂处理所述培养和任选扩增的细胞，使得所述细胞伴随地表达多种肿瘤相关抗原。 根据本发明的方法获得的细胞以及它们的细胞成分，无论是单独还是与所述细胞组合，都可用于预防和治疗组成型表达多种肿瘤相关的一种或多种的不同组织型的恶性肿瘤 在所述细胞中表达的抗原。 方便地，所述细胞和/或细胞组分是疫苗的形式。 所述疫苗相对于现有技术是有利的，因为它们同时表达多种/全部甲基化调节的肿瘤相关抗原。