公开(公告)号：US20110206732A1

公开(公告)日：2011-08-25

申请号：US12673750

申请日：2008-08-19

申请人： Giampiero Pescarmona ,  Antonio Ponzetto ,  Gianfranco Merizzi ,  Luisa Gennero

发明人： Giampiero Pescarmona ,  Antonio Ponzetto ,  Gianfranco Merizzi ,  Luisa Gennero

IPC分类号： A61K39/04 ,  A61K38/38 ,  A61K38/12 ,  A61P35/00

CPC分类号： A61K31/155 ,  A61K38/38

摘要： The present invention relates to albumin as an active ingredient in a medicament for the treatment of cancer and to a pharmaceutical composition comprising at least one anti-neoplastic agent and albumin at a concentration of at least 2% w/v of the composition, in a pharmaceutically acceptable vehicle. The anti-neoplastic agent is preferably hydroxyurea, preferably at concentration between 10 μM and 1 mM, more preferably between 10 μM and 100 μM. The pharmaceutical composition of the invention is effective in the therapeutic treatment of cancer and advantageously shows reduced side effects in comparison with the administration of the anti-neoplastic agent alone.

摘要翻译： 本发明涉及白蛋白作为用于治疗癌症的药物中的活性成分和包含至少一种浓度为组合物至少2％w / v的抗肿瘤剂和白蛋白的药物组合物，其中 药学上可接受的载体。 抗肿瘤剂优选为羟基脲，优选浓度为10μM至1mM，更优选10μM至100μM。 与单独使用抗肿瘤剂相比，本发明的药物组合物在治疗癌症方面是有效的，并且有利地显示减少的副作用。

公开(公告)号：US20100310544A1

公开(公告)日：2010-12-09

申请号：US12745253

申请日：2008-10-27

申请人： Luisa Gennero ,  Chiara Cesano ,  Carlo Vercelli ,  Antonio Ponzetto ,  Gianfranco Merizzi

发明人： Luisa Gennero ,  Chiara Cesano ,  Carlo Vercelli ,  Antonio Ponzetto ,  Gianfranco Merizzi

IPC分类号： A61K38/51 ,  A61K38/48 ,  A61P17/02 ,  A61P43/00 ,  A61Q19/08 ,  A61Q19/00 ,  C12N5/07

CPC分类号： C12N5/0629 ,  A01N1/02 ,  A01N1/0226 ,  A61K9/0019 ,  A61K9/0043 ,  A61K9/006 ,  A61K9/0063 ,  A61K33/06 ,  A61K38/4873 ,  A61K45/06 ,  C12N5/0018 ,  C12N2500/14 ,  C12N2500/38 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/70 ,  C12N2501/905 ,  A61K2300/00

摘要： The invention is based on the finding that calcium ions and proteolytic enzymes, particularly papain, act in a synergetic way in the regeneration of skin and connective tissues. Thus, such a combination of active principles can be used for the preparation of culture media for cutaneous and/or connective tissue biopsies, which allow for the maintenance of the biopsies under viable conditions for periods of over 6 months, even for over 3 years, as well as for the preparation of pharmaceutical compositions or medical devices for the treatment of diseases in which the regeneration of cutaneous and connective tissues is beneficial (for instance, injuries and wounds, keloids, paradontopathies, conjunctivitis, nasal and ear polyps, cellulite, human and animal dermatitis), and for the preparation of cosmetic compositions that in particular have an anti-ageing effect on the skin.

摘要翻译： 本发明基于以下发现：钙离子和蛋白水解酶，特别是木瓜蛋白酶，在皮肤和结缔组织的再生中以协同的方式起作用。 因此，这种活性成分的组合可用于制备用于皮肤和/或结缔组织活组织检查的培养基，其允许在可行条件下维持6个月以上甚至3年以上的活检， 以及用于治疗皮肤和结缔组织再生有益的疾病的药物组合物或医疗装置的制备（例如伤口和伤口，瘢痕疙瘩，paradontopathies，结膜炎，鼻和耳息肉，脂肪团，人 和动物皮炎），以及用于制备特别对皮肤具有抗衰老作用的化妆品组合物。

公开(公告)号：US20090181007A1

公开(公告)日：2009-07-16

申请号：US12296850

申请日：2007-04-12

申请人： Luisa Gennero ,  Antonio Ponzetto ,  Enrico De Vivo ,  Luciano Contu ,  Emanuella Morra ,  Chiara Cesano ,  Giovanni Cavallo ,  Giuseppe Cicero ,  Gian Piero Pescarmona ,  Tetyana Denysenko ,  Maria Augusta Roos ,  Maurizio Sara

发明人： Luisa Gennero ,  Antonio Ponzetto ,  Enrico De Vivo ,  Luciano Contu ,  Emanuella Morra ,  Chiara Cesano ,  Giovanni Cavallo ,  Giuseppe Cicero ,  Gian Piero Pescarmona ,  Tetyana Denysenko ,  Maria Augusta Roos ,  Maurizio Sara

IPC分类号： A61K38/48 ,  C12N5/00 ,  C12N5/02 ,  C12N9/00 ,  C12N9/50 ,  C12N9/48 ,  C12N9/22 ,  C12N9/66 ,  C12N9/68 ,  C12N9/52 ,  C12N9/62 ,  A61K38/46 ,  A61P43/00

CPC分类号： C12N5/0655 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2501/15 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2502/1358

摘要： A composition for in vitro use as a culture medium or in vivo use as a pharmaceutical composition or a medical device, capable of accelerating the differentiation of stem cells into cells with a chondrocytic phenotype and of restoring the original trophism of chondrocytes, is described. The composition comprises, in combination, at least one proteolytic enzyme, at least one growth factor and at least one from a sugar, an amino acid, a vitamin factor, a vitamin, a nucleotide and a nucleoside, in a physiologically acceptable carrier or diluent. A method of differentiating stem cells in cells having a chondrocytic phenotype, the cells obtained by the method and their uses, for example in human or animal cell therapy, for example by CBMP (Cellular Based Medicinal products) are also described.

摘要翻译： 描述了用于体外用作培养基或体内用作药物组合物或医疗装置的组合物，其能够加速干细胞分化成具有软骨细胞表型的细胞并恢复软骨细胞的原始营养。 组合物在生理上可接受的载体或稀释剂中组合包含至少一种蛋白水解酶，至少一种生长因子和至少一种来自糖，氨基酸，维生素因子，维生素，核苷酸和核苷的组合物 。 还描述了在具有软骨细胞表型的细胞中分化干细胞的方法，通过该方法获得的细胞及其用途，例如在人或动物细胞治疗中，例如通过CBMP（基于细胞的药物产品）。

公开(公告)号：US20080317730A1

公开(公告)日：2008-12-25

申请号：US12093112

申请日：2006-11-10

申请人： Luisa Gennero ,  Antonio Ponzetto ,  Enrico De Vivo ,  Luciano Contu ,  Emanuella Morra ,  Chiara Cesano

发明人： Luisa Gennero ,  Antonio Ponzetto ,  Enrico De Vivo ,  Luciano Contu ,  Emanuella Morra ,  Chiara Cesano

IPC分类号： A61K38/45 ,  A61K38/43 ,  A61K38/48 ,  A61P17/00 ,  A61K38/46

CPC分类号： A61K38/48 ,  A61K8/447 ,  A61K8/66 ,  A61K8/67 ,  A61K31/07 ,  A61K31/198 ,  A61K31/375 ,  A61K31/4188 ,  A61K38/063 ,  A61K38/39 ,  A61K45/06 ,  A61Q5/065 ,  A61K2300/00

摘要： The present invention relates to compositions and products comprising at least one proteolytic enzyme adapted to promoting the restoration of original trophism of the integumentary apparatus, the original pigmentation and stimulating the growth thereof and, particularly, improving the viability of the pilifer follicles, even in scar tissue areas.

摘要翻译： 本发明涉及包含至少一种蛋白水解酶的组合物和产品，所述蛋白水解酶适于促进皮肤器官的原始营养的恢复，原始色素沉着和刺激其生长，并且特别是改善毛囊的生存力，甚至在疤痕 组织区域。

公开(公告)号：US20110045519A1

公开(公告)日：2011-02-24

申请号：US12521517

申请日：2007-12-21

申请人： Luisa Gennero ,  Antonio Ponzetto ,  Giampiero Pescarmona ,  Andrea Savarino ,  Gianfranco Merizzi

发明人： Luisa Gennero ,  Antonio Ponzetto ,  Giampiero Pescarmona ,  Andrea Savarino ,  Gianfranco Merizzi

IPC分类号： C12N5/071 ,  C12N5/22 ,  C12P21/00 ,  C12Q1/02

CPC分类号： C12N5/067 ,  C12N5/0694 ,  C12N5/16 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/11 ,  C12N2501/12 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/39 ,  C12N2501/805 ,  C12N2502/11 ,  C12N2502/30

摘要： The invention relates to cell lines from differentiated cells with hepatocytic phenotypes capable of producing albumin and blood coagulation factors, said cells being derived from a human leukaemia cell line, preferably the human THP1 cell line, and preserving the characteristics of immortality. Among the cell lines of the invention, the cell lines known as PSC-THP1-EP, PSC-THP1-EP-FAST, PSC-THP1-HEP and PSC-THP1-EPEP are preferred. The invention also relates to methods for obtaining the cell lines of the invention and the uses of said cell lines, particularly for the production of albumin and/or blood coagulation factors.

摘要翻译： 本发明涉及能够产生白蛋白和血液凝固因子的肝细胞表型的分化细胞的细胞系，所述细胞衍生自人白血病细胞系，优选人THP1细胞系，并保持不朽的特征。 在本发明的细胞系中，优选称为PSC-THP1-EP，PSC-THP1-EP-FAST，PSC-THP1-HEP和PSC-THP1-EPEP的细胞系。 本发明还涉及获得本发明的细胞系和所述细胞系的用途，特别是用于产生白蛋白和/或凝血因子的方法。

公开(公告)号：US20100130544A1

公开(公告)日：2010-05-27

申请号：US12301392

申请日：2007-02-28

申请人： Antonio Ponzetto ,  Luisa Gennero ,  Maria Augusta Roos ,  Gian piero Pescarmona ,  Tetyana Denysenko ,  Paolo Di Nardo ,  Roberto Sangiorgio

发明人： Antonio Ponzetto ,  Luisa Gennero ,  Maria Augusta Roos ,  Gian piero Pescarmona ,  Tetyana Denysenko ,  Paolo Di Nardo ,  Roberto Sangiorgio

IPC分类号： A61K31/4748 ,  C12N5/071 ,  A61K31/44 ,  A61K31/22 ,  A61P35/00

CPC分类号： C12N5/067 ,  A61K31/46 ,  C12N2501/01 ,  C12N2501/12 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/235 ,  C12N2501/805 ,  C12N2506/115 ,  C12N2506/1353 ,  C12N2506/1392

摘要： The invention refers to the in vitro and in vivo use of parasympatholytic substances, preferably scopolamine, to potentiate and accelerate the differentiation of stem cells into cells with a tissue-specific phenotype, and the process and compositions related thereto.

摘要翻译： 本发明涉及副溶血性物质（优选东莨菪碱）的体外和体内使用，以加强和加速干细胞分化成具有组织特异性表型的细胞，以及与其相关的方法和组合。

公开(公告)号：US20100092454A1

公开(公告)日：2010-04-15

申请号：US12520503

申请日：2007-12-20

申请人： Enrico De Vivo ,  Antonio Ponzetto ,  Chiara Cesano ,  Luisa Gennero ,  Gianfranco Merizzi ,  Emanuella Morra

发明人： Enrico De Vivo ,  Antonio Ponzetto ,  Chiara Cesano ,  Luisa Gennero ,  Gianfranco Merizzi ,  Emanuella Morra

IPC分类号： A61K38/43 ,  A61K38/46 ,  A61K38/48 ,  A61K36/38 ,  A61K31/55 ,  A61K31/4525 ,  A61K31/15 ,  A61K31/138 ,  A61K31/135

CPC分类号： A61K38/48 ,  A61K8/49 ,  A61K8/66 ,  A61K31/135 ,  A61K31/137 ,  A61K31/203 ,  A61K31/4525 ,  A61K31/55 ,  A61K36/38 ,  A61K38/4873 ,  A61K45/06 ,  A61K2800/70 ,  A61Q5/10 ,  A61Q7/00 ,  A61Q19/04 ,  A61Q19/08 ,  A61K2300/00

摘要： What is described is a use of an antidepressant compound, preferably belonging to the class of selective serotonin reuptake inhibitors (SSRI), for regenerating the integumentary system and/or for stimulating the growth, the original trophism and/or original pigmentation of the corresponding cutaneous appendages, particularly body hair and/or head hair, in mammals. For this purpose, the antidepressant can be formulated in a cosmetic preparation, a pharmaceutical composition, a medical device, or in the form of a culture medium, alone or in combination with a further active ingredient such as a proteolytic enzyme and/or a vitamin.

摘要翻译： 所描述的是使用抗抑郁化合物，优选属于选择性5-羟色胺再摄取抑制剂（SSRI）类别，用于再生皮肤系统和/或刺激生长，相应皮肤的原始营养和/或原始色素沉着 在哺乳动物中的附属物，特别是身体毛发和/或头发。 为此，抗抑郁药可以配制在化妆品制剂，药物组合物，医疗装置中，或以培养基的形式单独或与另外的活性成分如蛋白水解酶和/或维生素 。

公开(公告)号：US20080311625A1

公开(公告)日：2008-12-18

申请号：US12094147

申请日：2006-11-16

申请人： Luisa Gennero ,  Antonio Ponzetto ,  Andrea Savarino

发明人： Luisa Gennero ,  Antonio Ponzetto ,  Andrea Savarino

IPC分类号： C12P21/04 ,  C12N5/08

CPC分类号： C12N5/0694 ,  C12N5/067 ,  C12N2501/01 ,  C12N2501/12 ,  C12N2501/23 ,  C12N2501/235 ,  C12N2506/11 ,  C12N2506/30

摘要： The present invention relates to immortal pluripotent stem cells derived from a human leukaemia cell line, preferably a human monocytoid cell line and more preferably the human monocytoid cell line, THP1. The present invention further relates to cell lines derived from the immortal pluripotent stem cell line having the phenotype of cell strains characteristic of human tissues, particularly having a human hepatocyte phenotype, as well as the methods for preparing thereof. The present invention further relates to the use of the derived cell line with a human hepatocytic phenotype for the production of albumin and blood coagulation factors.

摘要翻译： 本发明涉及源自人白血病细胞系，优选人单核细胞系细胞系，更优选人单核细胞增生细胞系THP1的永生多能干细胞。 本发明还涉及源自具有人组织，特别是人肝细胞表型特征的细胞株表型的永生多能干细胞系的细胞系及其制备方法。 本发明还涉及具有人肝细胞表型的衍生细胞系用于产生白蛋白和凝血因子的用途。