公开(公告)号：US20090181007A1

公开(公告)日：2009-07-16

申请号：US12296850

申请日：2007-04-12

申请人： Luisa Gennero ,  Antonio Ponzetto ,  Enrico De Vivo ,  Luciano Contu ,  Emanuella Morra ,  Chiara Cesano ,  Giovanni Cavallo ,  Giuseppe Cicero ,  Gian Piero Pescarmona ,  Tetyana Denysenko ,  Maria Augusta Roos ,  Maurizio Sara

发明人： Luisa Gennero ,  Antonio Ponzetto ,  Enrico De Vivo ,  Luciano Contu ,  Emanuella Morra ,  Chiara Cesano ,  Giovanni Cavallo ,  Giuseppe Cicero ,  Gian Piero Pescarmona ,  Tetyana Denysenko ,  Maria Augusta Roos ,  Maurizio Sara

IPC分类号： A61K38/48 ,  C12N5/00 ,  C12N5/02 ,  C12N9/00 ,  C12N9/50 ,  C12N9/48 ,  C12N9/22 ,  C12N9/66 ,  C12N9/68 ,  C12N9/52 ,  C12N9/62 ,  A61K38/46 ,  A61P43/00

CPC分类号： C12N5/0655 ,  A61L27/3817 ,  A61L27/3852 ,  A61L27/3895 ,  A61L2430/06 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2501/15 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2502/1358

摘要： A composition for in vitro use as a culture medium or in vivo use as a pharmaceutical composition or a medical device, capable of accelerating the differentiation of stem cells into cells with a chondrocytic phenotype and of restoring the original trophism of chondrocytes, is described. The composition comprises, in combination, at least one proteolytic enzyme, at least one growth factor and at least one from a sugar, an amino acid, a vitamin factor, a vitamin, a nucleotide and a nucleoside, in a physiologically acceptable carrier or diluent. A method of differentiating stem cells in cells having a chondrocytic phenotype, the cells obtained by the method and their uses, for example in human or animal cell therapy, for example by CBMP (Cellular Based Medicinal products) are also described.

摘要翻译： 描述了用于体外用作培养基或体内用作药物组合物或医疗装置的组合物，其能够加速干细胞分化成具有软骨细胞表型的细胞并恢复软骨细胞的原始营养。 组合物在生理上可接受的载体或稀释剂中组合包含至少一种蛋白水解酶，至少一种生长因子和至少一种来自糖，氨基酸，维生素因子，维生素，核苷酸和核苷的组合物 。 还描述了在具有软骨细胞表型的细胞中分化干细胞的方法，通过该方法获得的细胞及其用途，例如在人或动物细胞治疗中，例如通过CBMP（基于细胞的药物产品）。

公开(公告)号：US20100130544A1

公开(公告)日：2010-05-27

申请号：US12301392

申请日：2007-02-28

申请人： Antonio Ponzetto ,  Luisa Gennero ,  Maria Augusta Roos ,  Gian piero Pescarmona ,  Tetyana Denysenko ,  Paolo Di Nardo ,  Roberto Sangiorgio

发明人： Antonio Ponzetto ,  Luisa Gennero ,  Maria Augusta Roos ,  Gian piero Pescarmona ,  Tetyana Denysenko ,  Paolo Di Nardo ,  Roberto Sangiorgio

IPC分类号： A61K31/4748 ,  C12N5/071 ,  A61K31/44 ,  A61K31/22 ,  A61P35/00

CPC分类号： C12N5/067 ,  A61K31/46 ,  C12N2501/01 ,  C12N2501/12 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/235 ,  C12N2501/805 ,  C12N2506/115 ,  C12N2506/1353 ,  C12N2506/1392

摘要： The invention refers to the in vitro and in vivo use of parasympatholytic substances, preferably scopolamine, to potentiate and accelerate the differentiation of stem cells into cells with a tissue-specific phenotype, and the process and compositions related thereto.

摘要翻译： 本发明涉及副溶血性物质（优选东莨菪碱）的体外和体内使用，以加强和加速干细胞分化成具有组织特异性表型的细胞，以及与其相关的方法和组合。