公开(公告)号：US20050059146A1

公开(公告)日：2005-03-17

申请号：US10922577

申请日：2004-08-19

申请人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

发明人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

IPC分类号： C12N5/02 ,  C12N5/071 ,  C12N5/09 ,  C12N5/06

CPC分类号： C12N5/0694 ,  C12N2500/90 ,  C12N2502/00 ,  C12N2502/30 ,  C12N2510/02

摘要： A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

摘要翻译： 用于选择克隆和重新克隆哺乳动物细胞的新方法，其对于生产生物药物，优选仓鼠或小鼠骨髓瘤细胞具有重要性，具有高度的自动化和生产能力。 本发明涉及沉积和复制所述细胞的单细胞克隆的方法。 本发明还涉及使用通过单细胞沉积获得和复制的细胞以及允许单细胞复制的组合物制备蛋白质的方法。

公开(公告)号：US08669109B2

公开(公告)日：2014-03-11

申请号：US12899914

申请日：2010-10-07

申请人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

发明人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

IPC分类号： C12N15/00 ,  C12N5/07 ,  C12N5/10 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0694 ,  C12N2500/90 ,  C12N2502/00 ,  C12N2502/30 ,  C12N2510/02

摘要： A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

摘要翻译： 用于选择克隆和重新克隆哺乳动物细胞的新方法，其对于生产生物药物，优选仓鼠或小鼠骨髓瘤细胞具有重要性，具有高度的自动化和生产能力。 本发明涉及沉积和复制所述细胞的单细胞克隆的方法。 本发明还涉及使用通过单细胞沉积获得和复制的细胞以及允许单细胞复制的组合物制备蛋白质的方法。

公开(公告)号：US07833787B2

公开(公告)日：2010-11-16

申请号：US10922577

申请日：2004-08-19

申请人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

发明人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

IPC分类号： C12N5/07 ,  C12N5/10 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0694 ,  C12N2500/90 ,  C12N2502/00 ,  C12N2502/30 ,  C12N2510/02

摘要： A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

摘要翻译： 用于选择克隆和重新克隆哺乳动物细胞的新方法，其对于生产生物药物，优选仓鼠或小鼠骨髓瘤细胞具有重要性，具有高度的自动化和生产能力。 本发明涉及沉积和复制所述细胞的单细胞克隆的方法。 本发明还涉及使用通过单细胞沉积获得和复制的细胞以及允许单细胞复制的组合物制备蛋白质的方法。

公开(公告)号：US20110230642A1

公开(公告)日：2011-09-22

申请号：US12922945

申请日：2009-03-16

申请人： Juergen Fieder ,  Lore Florin ,  Hitto Kaufmann ,  Thomas Krieg ,  Melanie Briegel

发明人： Juergen Fieder ,  Lore Florin ,  Hitto Kaufmann ,  Thomas Krieg ,  Melanie Briegel

IPC分类号： C12N5/071 ,  C12P21/00 ,  C07K2/00 ,  C12N5/09

CPC分类号： C12N5/0037 ,  C12N2500/84 ,  C12N2501/105 ,  C12N2502/243 ,  C12N2510/02

摘要： The present invention relates to the field of cell culture technology and relates to methods of replicating/cloning cells, preferably cell lines which are important for the production of biopharmaceuticals. The invention also relates to methods of preparing proteins using cells that have been obtained and replicated by single cell deposition and compositions which make it possible to replicate individual cells.By using IGF particularly in conjunction with HSA in the culture medium after recloning, the recloning efficiency and hence the quantity of clones obtained can be increased significantly.

摘要翻译： 本发明涉及细胞培养技术领域，涉及复制/克隆细胞的方法，优选细胞系对于生产生物药物的生产是重要的。 本发明还涉及使用已经通过单细胞沉积获得和复制的细胞制备蛋白质的方法，以及使得能够复制个体细胞的组合物。 通过使用IGF，特别是与再生后的培养基中的HSA结合，可以显着提高所获得的克隆的再生能力和因此的量。

公开(公告)号：US08597909B2

公开(公告)日：2013-12-03

申请号：US13062781

申请日：2009-09-09

申请人： Lore Florin ,  Rebecca Bischoff ,  Juergen Fieder ,  Hitto Kaufmann ,  Thomas Krieg

发明人： Lore Florin ,  Rebecca Bischoff ,  Juergen Fieder ,  Hitto Kaufmann ,  Thomas Krieg

IPC分类号： C12P21/06

CPC分类号： C12N5/0037 ,  C12N2500/30 ,  C12N2502/99 ,  C12N2510/02

摘要： The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types.

摘要翻译： 本发明涉及细胞培养技术的领域，涉及对生物药物生产重要的细胞，优选细胞系的繁殖/克隆方法。 本发明还涉及用于制造蛋白质的方法以及使用通过单细胞分选提取和倍增的细胞以及能够使单细胞增殖的培养基组合物的方法。 通过使用生产白蛋白，优选产生HSA的细胞作为培养基或宿主细胞调节的饲养细胞，可以显着提高获得的克隆效率，从而获得克隆的量。 这些方法的组合也是可能的。 通过使用产生白蛋白，优选产生HSA的细胞，可以在无血清和/或不含胰岛素的培养基以及不同细胞类型中实现再生能力的提高。

公开(公告)号：US20110262967A1

公开(公告)日：2011-10-27

申请号：US13062781

申请日：2009-09-09

申请人： Lore Florin ,  Rebecca Bischoff ,  Juergen Fieder ,  Hitto Kaufmann ,  Thomas Krieg

发明人： Lore Florin ,  Rebecca Bischoff ,  Juergen Fieder ,  Hitto Kaufmann ,  Thomas Krieg

IPC分类号： C12P21/00 ,  C12N5/071

CPC分类号： C12N5/0037 ,  C12N2500/30 ,  C12N2502/99 ,  C12N2510/02

摘要： The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types.

摘要翻译： 本发明涉及细胞培养技术的领域，涉及对生物药物生产重要的细胞，优选细胞系的繁殖/克隆方法。 本发明还涉及用于制造蛋白质的方法以及使用通过单细胞分选提取和倍增的细胞以及能够使单细胞增殖的培养基组合物的方法。 通过使用生产白蛋白，优选产生HSA的细胞作为培养基或宿主细胞调节的饲养细胞，可以显着提高获得的克隆效率，从而获得克隆的量。 这些方法的组合也是可能的。 通过使用产生白蛋白，优选产生HSA的细胞，可以在无血清和/或不含胰岛素的培养基以及不同细胞类型中实现再生能力的提高。

公开(公告)号：US20050133073A1

公开(公告)日：2005-06-23

申请号：US11012454

申请日：2004-12-15

申请人： Thomas Krieg

发明人： Thomas Krieg

IPC分类号： B66C1/42 ,  H01L21/68 ,  H01L21/687

CPC分类号： H01L21/68707

摘要： A substrate carrier (20, 30, 40, 50) for receiving and retaining a planar element in position. At least one plastic support (25) is provided on the substrate carrier, and the planar element is held exclusively by means of adhesion.

摘要翻译： 用于接收并保持平面元件就位的衬底载体（20,30,40,50）。 至少一个塑料支撑件（25）设置在基板载体上，并且平面元件仅通过粘合来保持。

公开(公告)号：US08563303B2

公开(公告)日：2013-10-22

申请号：US12922945

申请日：2009-03-16

申请人： Jüergen Fieder ,  Lore Florin ,  Hitto Kaufmann ,  Thomas Krieg ,  Melanie Briegel

发明人： Jüergen Fieder ,  Lore Florin ,  Hitto Kaufmann ,  Thomas Krieg ,  Melanie Briegel

IPC分类号： C12N5/02 ,  C12N5/07 ,  C12N5/18

CPC分类号： C12N5/0037 ,  C12N2500/84 ,  C12N2501/105 ,  C12N2502/243 ,  C12N2510/02

摘要： The present invention relates to the field of cell culture technology and relates to methods of replicating/cloning cells, preferably cell lines which are important for the production of biopharmaceuticals. The invention also relates to methods of preparing proteins using cells that have been obtained and replicated by single cell deposition and compositions which make it possible to replicate individual cells.By using IGF particularly in conjunction with HSA in the culture medium after recloning, the recloning efficiency and hence the quantity of clones obtained can be increased significantly.

摘要翻译： 本发明涉及细胞培养技术领域，涉及复制/克隆细胞的方法，优选细胞系对于生产生物药物的生产是重要的。 本发明还涉及使用已经通过单细胞沉积获得和复制的细胞制备蛋白质的方法，以及使得能够复制个体细胞的组合物。 通过使用IGF，特别是与再生后的培养基中的HSA结合，可以显着提高所获得的克隆的再生能力和因此的量。

公开(公告)号：US08168590B2

公开(公告)日：2012-05-01

申请号：US12349531

申请日：2009-01-07

申请人： Sabine Eming ,  Thomas Krieg ,  Stephan Sollberg ,  Gereon Lauer

发明人： Sabine Eming ,  Thomas Krieg ,  Stephan Sollberg ,  Gereon Lauer

IPC分类号： A61K38/18 ,  C07K14/475

CPC分类号： C07K14/52 ,  A61K38/1866

摘要： The invention relates to vascular endothelial growth factor (VEGF) in which the alanine at AA position 111 is replaced by proline. The arginine at AA position 110 may moreover be replaced by another amino acid. The invention also relates to derivatives of the VEGF according to the invention, nucleic acids, expression systems, medicaments and the use of the VEGF mutants of the invention for the treatment of chronic wounds.

摘要翻译： 本发明涉及其中AA位置111处的丙氨酸被脯氨酸替代的血管内皮生长因子（VEGF）。 AA位置110处的精氨酸可另外被另一氨基酸替代。 本发明还涉及根据本发明的VEGF的衍生物，核酸，表达系统，药物以及本发明的VEGF突变体用于治疗慢性伤口的用途。

公开(公告)号：US20060148687A1

公开(公告)日：2006-07-06

申请号：US10506893

申请日：2003-03-06

申请人： Sabine Eming ,  Thomas Krieg ,  Stephan Sollberg ,  Gereon Lauer

发明人： Sabine Eming ,  Thomas Krieg ,  Stephan Sollberg ,  Gereon Lauer

IPC分类号： A61K38/18 ,  C07K14/475

CPC分类号： C07K14/52 ,  A61K38/1866

摘要： The invention relates to endothelial growth factor (VEGF) in which the alanine at AA position 111 is replaced by proline. The arginine at AA position 110 may moreover be replaced by another amino acid. The invention also relates to derivatives of the VEGF according to the invention, nucleic acids, expression systems, medicaments and the use of the VEGF mutants of the invention for the treatment of chronic wounds.

摘要翻译： 本发明涉及其中AA位置111处的丙氨酸被脯氨酸替代的内皮生长因子（VEGF）。 AA位置110处的精氨酸可另外被另一氨基酸替代。 本发明还涉及根据本发明的VEGF的衍生物，核酸，表达系统，药物以及本发明的VEGF突变体用于治疗慢性伤口的用途。