公开(公告)号：US20240316196A1

公开(公告)日：2024-09-26

申请号：US18025590

申请日：2022-12-02

申请人： NUWACELL BIOTECHNOLOGIES CO., LTD.

发明人： Junying YU ,  Tao ZHOU ,  Changqing TIAN ,  Yali GAO ,  Ying ZHANG

IPC分类号： A61K39/00 ,  C12N5/0783

CPC分类号： A61K39/4613 ,  A61K39/464499 ,  C12N5/0646 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2302 ,  C12N2501/2303 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/26 ,  C12N2501/415 ,  C12N2502/00 ,  C12N2506/45

摘要： The present disclosure provides, inter alia, methods and compositions for differentiation of pluripotent stem cells and derived hematopoietic lineage cells including hemogenic endothelial cells, hematopoietic progenitor cells and natural killer cells. The differentiation efficiency for the hemogenic endothelial cells, the hematopoietic progenitor cells and the natural killer cells can be improved by using the methods and compositions of this disclosure described herein.

公开(公告)号：US20240299539A1

公开(公告)日：2024-09-12

申请号：US18256798

申请日：2021-12-16

申请人： Iovance Biotherapeutics, Inc.

发明人： Maria FARDIS ,  Friedrich Graf Finck FINCKENSTEIN ,  Zelanna GOLDBERG

IPC分类号： A61K39/00 ,  A61K39/395 ,  A61P35/00 ,  C12N5/0783

CPC分类号： A61K39/4611 ,  A61K39/395 ,  A61K39/464499 ,  A61P35/00 ,  C12N5/0636 ,  A61K2039/507 ,  A61K2239/39 ,  C12N2501/2302 ,  C12N2501/2315 ,  C12N2501/2321 ,  C12N2502/00

摘要： The present invention provides improved and/or shortened processes and methods for preparing TILs in order to prepare therapeutic populations of TILs with increased therapeutic efficacy for the treatment of cancer with TILs in combination with CT-LA-4 and PD-1 inhibitors and/or PD-L1 inhibitors as described herein.

公开(公告)号：US20240197873A1

公开(公告)日：2024-06-20

申请号：US18556511

申请日：2022-04-22

申请人： SUZHOU GRIT BIOTECHNOLOGY CO., LTD. ,  ZHUHAI TUOYU BIOTECHNOLOGY CO., LTD. ,  SHANGHAI GRIT BIOTECHNOLOGY CO., LTD. ,  ZHUHAI GRIT BIOTECHNOLOGY CO., LTD.

发明人： Yarong LIU ,  Jingwei SUN ,  Jiahui JIN

IPC分类号： A61K39/00 ,  A61P35/00 ,  C07K14/54 ,  C07K14/715 ,  C12N5/0783 ,  C12N15/86

CPC分类号： A61K39/4611 ,  A61K39/4635 ,  A61P35/00 ,  C07K14/5443 ,  C07K14/7155 ,  C12N5/0636 ,  C12N15/86 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2501/2302 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2502/00 ,  C12N2740/10043

摘要： Provided are modified tumor-infiltrating lymphocyte and the use thereof, in particular provided is a method for culturing the tumor-infiltrating lymphocyte (TIL), which comprises increasing the expression and/or enhancing the activity of at least one cytokine of the TIL. Also provided is a method for preventing and/or treating tumors by using the tumor-infiltrating lymphocyte.

公开(公告)号：US20230220340A1

公开(公告)日：2023-07-13

申请号：US18147981

申请日：2022-12-29

申请人： Providence Health & Services - Oregon ,  AgonOx, Inc.

发明人： Andrew D. Weinberg ,  Ryan Montler ,  Thomas Duhen ,  Rebekka Duhen

IPC分类号： C12N5/0783 ,  A61K35/17 ,  C07K14/725 ,  C12N15/10 ,  C12N15/85 ,  A61K48/00

CPC分类号： C12N5/0636 ,  A61K35/17 ,  C07K14/7051 ,  C12N15/1003 ,  C12N15/85 ,  C12N2501/2315 ,  C12N2502/00 ,  C12N2500/32 ,  A61K48/00

摘要： Methods are disclosed for treating a subject with a tumor. These methods include administering to the subject a therapeutically effective amount of CD8+CD39+CD103+ T cells. Methods also are disclosed for isolating a nucleic acid encoding a T cell receptor (TCR) that specifically binds a tumor cell antigen. These methods include isolating CD8+CD39+CD103+ T cells from a sample from a subject with a tumor expressing the tumor cell antigen, and cloning a nucleic acid molecule encoding a TCR from the CD8+CD39+CD103+ T cells. In addition, methods are disclosed for expanding CD8+CD39+CD103+ T cells. In additional embodiments, methods are disclosed for determining if a subject with a tumor will respond to a checkpoint inhibitor. The methods include detecting the presence of CD8+CD39+CD103+ T cells in a biological sample from a subject.

公开(公告)号：US09913864B2

公开(公告)日：2018-03-13

申请号：US14826613

申请日：2015-08-14

申请人： THE UNIVERSITY OF VERMONT AND STATE AGRICULTURE COLLEGE

发明人： Jeffrey Spees

IPC分类号： C12N5/077 ,  A61K35/34 ,  A61K38/18 ,  A61K38/30 ,  C12N5/071 ,  A61K38/19

CPC分类号： A61K35/34 ,  A61K38/1833 ,  A61K38/1866 ,  A61K38/195 ,  A61K38/30 ,  C12N5/0657 ,  C12N5/069 ,  C12N2501/10 ,  C12N2501/105 ,  C12N2501/12 ,  C12N2501/165 ,  C12N2502/00

摘要： The invention features compositions comprising agents having cardiac protective activity isolated from epicardial progenitor cells and derivatives thereof, and methods for the use of such compositions.

公开(公告)号：US20170340670A1

公开(公告)日：2017-11-30

申请号：US15666472

申请日：2017-08-01

申请人： CARMEL-HAIFA UNIVERSITY ECONOMIC CORPORATION LTD

发明人： Dalit Barkan ,  Odelya Gilon ,  Amiram Ariel ,  Sagie Schif-Zuck ,  Aroesti-Assi Simaan

IPC分类号： A61K35/15 ,  A61K35/12 ,  C12N5/077 ,  A61K45/06 ,  C12N5/0786

CPC分类号： A61K35/15 ,  A61K35/12 ,  A61K45/06 ,  C12N5/0645 ,  C12N5/0656 ,  C12N2500/70 ,  C12N2501/15 ,  C12N2501/58 ,  C12N2502/00 ,  C12N2502/1157 ,  C12N2506/11 ,  C12N2506/115 ,  C12N2506/1307

摘要： A conditioned cell culture medium of CD11blow human macrophages or a biologically active fraction thereof can be prepared by a method that includes (i) culturing a population of human mononuclear cells of the monocyte/macrophage lineage for 5-7 days, so as to induce differentiation of the mononuclear cells to macrophages; (ii) incubating the macrophages obtained in (i) with apoptotic cells or in the presence of a pro-resolving lipid mediator to reduce the CD11b expression, thus obtaining a culture of CD11blow macrophages; and (iii) collecting the conditioned cell culture medium of CD11blow macrophages. Pharmaceutical compositions containing the CD11blow macrophages conditioned medium or a culture of CD11blow macrophages can be used in the treatment of cancer or fibrosis.

公开(公告)号：US09132155B2

公开(公告)日：2015-09-15

申请号：US13220555

申请日：2011-08-29

申请人： Jeffrey Spees

发明人： Jeffrey Spees

IPC分类号： A61K35/34 ,  C12N5/077 ,  A61K38/18 ,  A61K38/30 ,  C12N5/071 ,  A61K38/19

CPC分类号： A61K35/34 ,  A61K38/1833 ,  A61K38/1866 ,  A61K38/195 ,  A61K38/30 ,  C12N5/0657 ,  C12N5/069 ,  C12N2501/10 ,  C12N2501/105 ,  C12N2501/12 ,  C12N2501/165 ,  C12N2502/00

摘要： The invention features compositions comprising agents having cardiac protective activity isolated from epicardial progenitor cells and derivatives thereof, and methods for the use of such compositions.

摘要翻译： 本发明的特征在于包含具有从心外膜祖细胞及其衍生物分离的心脏保护活性的试剂的组合物，以及使用这些组合物的方法。

公开(公告)号：US20110111500A1

公开(公告)日：2011-05-12

申请号：US12590464

申请日：2009-11-09

申请人： Stephen Iiye Chen

发明人： Stephen Iiye Chen

IPC分类号： C12N5/00

CPC分类号： C12N5/0075 ,  C12M23/12 ,  C12M25/16 ,  C12N2502/00 ,  C12N2513/00

摘要： This invention provides a co-culture method using 3-dimensional feeders to support single cell in microwells of microarray chips. Microbeads are utilized as carrier to manipulate feeders into 3-dimensional layers in a microwell, to retain feeders at desired location, to keep feeders away from single cell at a desired distance, to revive feeders for optimized co-culture, and to eliminate feeders from image background in post imaging analysis.

摘要翻译： 本发明提供一种使用三维进料器来支持微阵列微孔中的单细胞的共培养方法。 将微珠用作载体以将进料器操纵在微孔中的3维层中，以将进料器保持在期望的位置，以将进料器保持在期望距离处的单个细胞，以恢复进料器以优化共培养，并且消除来自 后成像分析中的图像背景。

公开(公告)号：US20050059146A1

公开(公告)日：2005-03-17

申请号：US10922577

申请日：2004-08-19

申请人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

发明人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

IPC分类号： C12N5/02 ,  C12N5/071 ,  C12N5/09 ,  C12N5/06

CPC分类号： C12N5/0694 ,  C12N2500/90 ,  C12N2502/00 ,  C12N2502/30 ,  C12N2510/02

摘要： A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

摘要翻译： 用于选择克隆和重新克隆哺乳动物细胞的新方法，其对于生产生物药物，优选仓鼠或小鼠骨髓瘤细胞具有重要性，具有高度的自动化和生产能力。 本发明涉及沉积和复制所述细胞的单细胞克隆的方法。 本发明还涉及使用通过单细胞沉积获得和复制的细胞以及允许单细胞复制的组合物制备蛋白质的方法。

公开(公告)号：US12037607B2

公开(公告)日：2024-07-16

申请号：US16762447

申请日：2018-11-08

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Raul E. Vizcardo ,  Naritaka Tamaoki ,  Meghan L. Good ,  Nicholas P. Restifo

IPC分类号： C12N5/0789 ,  A61K35/28

CPC分类号： C12N5/0647 ,  A61K35/28 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/135 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/20 ,  C12N2501/22 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/24 ,  C12N2501/25 ,  C12N2501/415 ,  C12N2502/00 ,  C12N2506/45 ,  C12N2513/00 ,  C12N2531/00

摘要： Disclosed are methods of preparing CD34+CD43+ hematopoietic progenitor cells (HPC) in vitro according to embodiments of the invention. Also disclosed are methods of differentiating CD34+CD43+ hematopoietic progenitor cells to hematopoietic lineage cells according to embodiments of the invention. Also disclosed are methods of treating or preventing a condition in a mammal, e.g., cancer, according to embodiments of the invention.