公开(公告)号：US08669109B2

公开(公告)日：2014-03-11

申请号：US12899914

申请日：2010-10-07

申请人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

发明人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

IPC分类号： C12N15/00 ,  C12N5/07 ,  C12N5/10 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0694 ,  C12N2500/90 ,  C12N2502/00 ,  C12N2502/30 ,  C12N2510/02

摘要： A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

摘要翻译： 用于选择克隆和重新克隆哺乳动物细胞的新方法，其对于生产生物药物，优选仓鼠或小鼠骨髓瘤细胞具有重要性，具有高度的自动化和生产能力。 本发明涉及沉积和复制所述细胞的单细胞克隆的方法。 本发明还涉及使用通过单细胞沉积获得和复制的细胞以及允许单细胞复制的组合物制备蛋白质的方法。

公开(公告)号：US20060245965A1

公开(公告)日：2006-11-02

申请号：US11391066

申请日：2006-03-28

申请人： Joachim Wienecke ,  Thomas Krieg

发明人： Joachim Wienecke ,  Thomas Krieg

IPC分类号： F04B39/00

CPC分类号： G01N21/9501 ,  G01N21/9503 ,  G01N2021/8427

摘要： An apparatus for inspecting a disk-like object comprising at least one first module for inspecting a surface of the disk-like object and at least one second module insertable in the apparatus. The at least one second module is arranged to inspect a different element of the disk-like object than the surface of the disk-like object.

公开(公告)号：US20060097438A1

公开(公告)日：2006-05-11

申请号：US11265872

申请日：2005-11-03

申请人： Thomas Krieg

发明人： Thomas Krieg

IPC分类号： B25B1/02 ,  B25B5/02

CPC分类号： H01L21/68707

摘要： The present invention relates to an adapter apparatus for a substrate workstation. The substrate workstation comprises at least one substrate carrier that receives from one side in substantially centered fashion, for transport and/or for handling, a substrate to be processed with the substrate workstation. The intention is to allow substrates that permit contamination only in the edge region also to be processed, in the simplest possible fashion, using a substrate workstation embodied for the centered reception of substrates. The adapter apparatus according to the present invention is characterized in that the adapter apparatus is reversibly adaptable to a substrate carrier of the substrate workstation. The adapter apparatus is embodied in such a way that the substrate is receivable only substantially at its edge region by the adapter apparatus. The present invention further relates to a substrate workstation.

摘要翻译： 本发明涉及一种用于衬底工作站的适配器装置。 衬底工作站包括至少一个衬底载体，其以基本中心的方式从一侧接收用于运输和/或处理要用衬底工作站处理的衬底。 目的是允许仅在边缘区域中允许污染的基板以最简单的可能方式使用实施用于基板中心接收的基板工作站进行加工。 根据本发明的适配器装置的特征在于，适配器装置可逆地适应于基板工作站的基板载体。 适配器装置以这样一种方式实现，即基板仅通过适配器装置基本上在其边缘区域处可接收。 本发明还涉及衬底工作站。

公开(公告)号：US20050219521A1

公开(公告)日：2005-10-06

申请号：US11076620

申请日：2005-03-10

申请人： Wolfgang Vollrath ,  Thomas Krieg

发明人： Wolfgang Vollrath ,  Thomas Krieg

IPC分类号： G01N21/956 ,  G01N21/00 ,  G01N21/95 ,  G01R31/26 ,  G02B27/02 ,  H01L21/66 ,  H01L21/68

CPC分类号： G01N21/9501

摘要： Examination devices and methods operating with incident light have hitherto been used for the examination of wafers. To allow these devices also to be used with the transmitted-light method, it is proposed to configure the substrate holder (16) so that an illumination device (38, 40, 42) is integrated into the substrate holder (16) in such a way that transmitted-light illumination of the wafer (18) is possible.

摘要翻译： 迄今为止，使用入射光操作的检查装置和方法用于检查晶片。 为了使这些装置也能够与透射光方法一起使用，建议将衬底保持器（16）配置成使得照明装置（38,40,42）以这样的方式集成到衬底保持器（16）中 使得晶片（18）的透射光照射是可能的。

公开(公告)号：US08597909B2

公开(公告)日：2013-12-03

申请号：US13062781

申请日：2009-09-09

申请人： Lore Florin ,  Rebecca Bischoff ,  Juergen Fieder ,  Hitto Kaufmann ,  Thomas Krieg

发明人： Lore Florin ,  Rebecca Bischoff ,  Juergen Fieder ,  Hitto Kaufmann ,  Thomas Krieg

IPC分类号： C12P21/06

CPC分类号： C12N5/0037 ,  C12N2500/30 ,  C12N2502/99 ,  C12N2510/02

摘要： The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types.

摘要翻译： 本发明涉及细胞培养技术的领域，涉及对生物药物生产重要的细胞，优选细胞系的繁殖/克隆方法。 本发明还涉及用于制造蛋白质的方法以及使用通过单细胞分选提取和倍增的细胞以及能够使单细胞增殖的培养基组合物的方法。 通过使用生产白蛋白，优选产生HSA的细胞作为培养基或宿主细胞调节的饲养细胞，可以显着提高获得的克隆效率，从而获得克隆的量。 这些方法的组合也是可能的。 通过使用产生白蛋白，优选产生HSA的细胞，可以在无血清和/或不含胰岛素的培养基以及不同细胞类型中实现再生能力的提高。

公开(公告)号：US20120258915A1

公开(公告)日：2012-10-11

申请号：US13445016

申请日：2012-04-12

申请人： Sabine Eming ,  Thomas Krieg ,  Stephan Sollberg ,  Gereon Lauer

发明人： Sabine Eming ,  Thomas Krieg ,  Stephan Sollberg ,  Gereon Lauer

IPC分类号： A61K38/18 ,  A61P17/02

CPC分类号： C07K14/52 ,  A61K38/1866

摘要： The invention relates to endothelial growth factor (VEGF) in which the alanine at AA position 111 is replaced by proline. The arginine at AA position 110 may moreover be replaced by another amino acid. The invention also relates to derivatives of the VEGF according to the invention, nucleic acids, expression systems, medicaments and the use of the VEGF mutants of the invention for the treatment of chronic wounds.

摘要翻译： 本发明涉及其中AA位置111处的丙氨酸被脯氨酸替代的内皮生长因子（VEGF）。 AA位置110处的精氨酸可另外被另一氨基酸替代。 本发明还涉及根据本发明的VEGF的衍生物，核酸，表达系统，药物以及本发明的VEGF突变体用于治疗慢性伤口的用途。

公开(公告)号：US20110262967A1

公开(公告)日：2011-10-27

申请号：US13062781

申请日：2009-09-09

申请人： Lore Florin ,  Rebecca Bischoff ,  Juergen Fieder ,  Hitto Kaufmann ,  Thomas Krieg

发明人： Lore Florin ,  Rebecca Bischoff ,  Juergen Fieder ,  Hitto Kaufmann ,  Thomas Krieg

IPC分类号： C12P21/00 ,  C12N5/071

CPC分类号： C12N5/0037 ,  C12N2500/30 ,  C12N2502/99 ,  C12N2510/02

摘要： The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types.

摘要翻译： 本发明涉及细胞培养技术的领域，涉及对生物药物生产重要的细胞，优选细胞系的繁殖/克隆方法。 本发明还涉及用于制造蛋白质的方法以及使用通过单细胞分选提取和倍增的细胞以及能够使单细胞增殖的培养基组合物的方法。 通过使用生产白蛋白，优选产生HSA的细胞作为培养基或宿主细胞调节的饲养细胞，可以显着提高获得的克隆效率，从而获得克隆的量。 这些方法的组合也是可能的。 通过使用产生白蛋白，优选产生HSA的细胞，可以在无血清和/或不含胰岛素的培养基以及不同细胞类型中实现再生能力的提高。

公开(公告)号：US20110009316A1

公开(公告)日：2011-01-13

申请号：US12349531

申请日：2009-01-07

申请人： Sabine EMING ,  Thomas KRIEG ,  Stephan SOLLBERG ,  Gereon LAUER

发明人： Sabine EMING ,  Thomas KRIEG ,  Stephan SOLLBERG ,  Gereon LAUER

IPC分类号： A61K38/18 ,  A61P7/06 ,  A61P29/00 ,  A61P35/00 ,  A61P7/00 ,  A61P17/00 ,  A61P3/00 ,  A61P7/10 ,  A61P9/00 ,  A61P3/10 ,  A61P19/06 ,  A61P1/04 ,  A61P35/04

CPC分类号： C07K14/52 ,  A61K38/1866

摘要： The invention relates to endothelial growth factor (VEGF) in which the alanine at AA position 111 is replaced by proline. The arginine at AA position 110 may moreover be replaced by another amino acid. The invention also relates to derivatives of the VEGF according to the invention, nucleic acids, expression systems, medicaments and the use of the VEGF mutants of the invention for the treatment of chronic wounds.

摘要翻译： 本发明涉及内皮生长因子（VEGF），其中AA位置111处的丙氨酸被脯氨酸替代。 AA位置110处的精氨酸可另外被另一氨基酸替代。 本发明还涉及根据本发明的VEGF的衍生物，核酸，表达系统，药物以及本发明的VEGF突变体用于治疗慢性伤口的用途。

公开(公告)号：US07833787B2

公开(公告)日：2010-11-16

申请号：US10922577

申请日：2004-08-19

申请人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

发明人： Ralf Otto ,  Barbara Enenkel ,  Juergen Fieder ,  Thomas Krieg

IPC分类号： C12N5/07 ,  C12N5/10 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N5/0694 ,  C12N2500/90 ,  C12N2502/00 ,  C12N2502/30 ,  C12N2510/02

摘要： A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.

摘要翻译： 用于选择克隆和重新克隆哺乳动物细胞的新方法，其对于生产生物药物，优选仓鼠或小鼠骨髓瘤细胞具有重要性，具有高度的自动化和生产能力。 本发明涉及沉积和复制所述细胞的单细胞克隆的方法。 本发明还涉及使用通过单细胞沉积获得和复制的细胞以及允许单细胞复制的组合物制备蛋白质的方法。

公开(公告)号：US07491696B2

公开(公告)日：2009-02-17

申请号：US10506893

申请日：2003-03-06

申请人： Sabine Eming ,  Thomas Krieg ,  Stephan Sollberg ,  Gereon Lauer

发明人： Sabine Eming ,  Thomas Krieg ,  Stephan Sollberg ,  Gereon Lauer

IPC分类号： A61K38/18 ,  C07K14/475 ,  C12N15/18 ,  C12N15/63

CPC分类号： C07K14/52 ,  A61K38/1866

摘要： The invention relates to endothelial growth factor (VEGF) in which the alanine at AA position 111 is replaced by proline. The arginine at AA position 110 may moreover be replaced by another amino acid. The invention also relates to derivatives of the VEGF according to the invention, nucleic acids, expression systems, medicaments and the use of the VEGF mutants of the invention for the treatment of chronic wounds.

摘要翻译： 本发明涉及其中AA位置111处的丙氨酸被脯氨酸替代的内皮生长因子（VEGF）。 AA位置110处的精氨酸可另外被另一氨基酸替代。 本发明还涉及根据本发明的VEGF的衍生物，核酸，表达系统，药物以及本发明的VEGF突变体用于治疗慢性伤口的用途。