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    Yeast host, transformant and method for producing heterologous proteins
    5.
    发明授权
    Yeast host, transformant and method for producing heterologous proteins 有权
    酵母宿主，转化体和产生异源蛋白质的方法

    公开(公告)号：US08329448B2

    公开(公告)日：2012-12-11

    申请号：US12025138

    申请日：2008-02-04

    申请人： Alimjan Idiris ,  Hideki Tohda ,  Yuko Hama

    发明人： Alimjan Idiris ,  Hideki Tohda ,  Yuko Hama

    IPC分类号： C12N1/16 ,  C12N1/19 ,  C12N15/09 ,  C12N15/12 ,  C12N15/16 ,  C12N15/18 ,  C12N15/81

    CPC分类号： C12N15/815 ,  C07K14/61 ,  C12P21/02

    摘要： The efficiency of production of a heterologous protein by a transformant of a yeast host is improved.A method of constructing a host for expression of a foreign gene which comprises deleting or inactivating at least one gene selected from the protease-associated genes (especially, metalloprotease genes and serine protease genes) of Schizosaccharomyces pombe, a host in which the above-mentioned gene is deleted or inactivated, a transformant obtained by introducing a foreign gene into the host and a method of producing a heterologous protein using the transformant.The protease-associated gene is at least one gene selected from the group consisting of psp3 (SPAC1006.01), is sxa2 (SPAC1296.03c), ppp51 (SPAC22G7.01c) and ppp52 (SPBC18A7.01) or at least two genes selected from the group consisting of metalloprotease genes and serine protease genes, especially, three genes psp3 (SPAC1006.01), isp6 (SPAC4A8.04) and ppp53 (SPAP14E8.04). A host in which four genes consisting of the above three genes and ppp16 (SPBC1711.12), or five genes consisting of the above four genes and ppp22 (SPBC14C8.03) are deleted or inactivated is preferred.

    摘要翻译： 通过酵母宿主的转化体产生异源蛋白质的效率得到改善。 构建用于表达外源基因的宿主的方法，其包括删除或失活选自粟酒裂殖酵母的蛋白酶相关基因（特别是金属蛋白酶基因和丝氨酸蛋白酶基因）中的至少一种基因，其中上述 基因被缺失或失活，通过将外源基因导入宿主获得的转化体和使用转化体产生异源蛋白质的方法。 蛋白酶相关基因是选自psp3（SPAC1006.01），sxa2（SPAC1296.03c），ppp51（SPAC22G7.01c）和ppp52（SPBC18A7.01）中的至少一种基因或选自至少两种基因 来自由金属蛋白酶基因和丝氨酸蛋白酶基因组成的组，特别是3个基因psp3（SPAC1006.01），isp6（SPAC4A8.04）和ppp53（SPAP14E8.04）。 优选缺失或失活的由以上三种基因组成的四种基因和ppp16（SPBC1711.12）或由上述四种基因组成的五种基因和ppp22（SPBC14C8.03）的宿主。

    hGH AND METHODS FOR PREPARING hGH
    7.
    发明申请
    hGH AND METHODS FOR PREPARING hGH 审中-公开
    hGH和制备hGH的方法

    公开(公告)号：US20110237509A1

    公开(公告)日：2011-09-29

    申请号：US13029961

    申请日：2011-02-17

    申请人： Matthew M. Skinner ,  Ronald Zimmerman

    发明人： Matthew M. Skinner ,  Ronald Zimmerman

    IPC分类号： A61K38/27 ,  C12N15/18 ,  C07K14/61 ,  C12N1/21 ,  C12N15/63 ,  A61P5/00

    CPC分类号： C07K14/61 ,  C12P21/06

    摘要： Preparations of recombinant human growth hormone (hGH) are provided having a purity that comprises 2% or less of a peptide other than native human growth hormone (e.g., essentially free of multimeric forms) are disclosed. Active pharmaceutical ingredient (API) preparations of recombinant hGH suitable for commercial production of formulation grade recombinant hGH are provided. A stable Master Cell Bank of transformed E. coli is also disclosed, as well as methods for creating a transformed E. coli preparation that includes a Met-AsphGH encoding sequence. Improved manufacturing methods are also presented that are suitable for large scale production of highly purified preparations of recombinant human growth hormone.

    摘要翻译： 提供重组人生长激素（hGH）的制备，所述重组人生长激素（hGH）的纯度包括比天然人生长激素（例如，基本上不含多聚体形式）2％或更少的肽。 提供了适用于制剂级重组hGH的商业生产的重组hGH的活性药物成分（API）制剂。 还公开了转化的大肠杆菌的稳定的主细胞库，以及用于产生包含Met-AsphGH编码序列的转化的大肠杆菌制剂的方法。 还提出了适用于大规模生产重组人生长激素高度纯化制剂的改进的制备方法。

    SYNTHETIC ANALOGS OF THE JUXTAMEMBRANE DOMAIN OF IGF1R AND USES THEREOF
    8.
    发明申请
    SYNTHETIC ANALOGS OF THE JUXTAMEMBRANE DOMAIN OF IGF1R AND USES THEREOF 审中-公开
    IGF1R的JUXTAMEMBRARE域的合成模拟及其用途

    公开(公告)号：US20110195911A1

    公开(公告)日：2011-08-11

    申请号：US12934911

    申请日：2009-03-27

    申请人： Nadya I. Tarasova ,  Sergey G. Tarasov

    发明人： Nadya I. Tarasova ,  Sergey G. Tarasov

    IPC分类号： A61K38/16 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C12N15/18 ,  C07K16/22 ,  A61K31/7052 ,  A61K38/10 ,  A61K38/08 ,  C12N5/00 ,  A61P35/00

    CPC分类号： C07K14/72

    摘要： A peptide or peptidomimetic comprising the amino acid sequence RXGNGV (SEQ ID NO: 1) or the inverse thereof, or comprising at least six contiguous amino acids of the juxtamembrane domain of IGF1R (SEQ ID NO: 43) or inverse thereof, wherein the peptide or peptidomimetic comprises a total of about 50 or fewer amino acids and inhibits IFG-R1 activity, as well as a method of inhibiting a IGF1R in a cell, a method of treating or preventing IGF1R-mediated disease, and related compounds, compositions, and methods.

    摘要翻译： 包含氨基酸序列RXGNGV（SEQ ID NO：1）或其逆向或包含IGF1R（SEQ ID NO：43）的并置结构域的至少六个相邻氨基酸或其逆转的肽或肽模拟物，其中所述肽 或肽模拟物包含总共约50个或更少的氨基酸并且抑制IFG-R1活性，以及​​抑制细胞中IGF1R的方法，治疗或预防IGF1R介导的疾病的方法，以及相关化合物，组合物和 方法。