A FLOW ELECTROTRANSFECTION DEVICE
    14.
    发明公开

    公开(公告)号:US20240067912A1

    公开(公告)日:2024-02-29

    申请号:US18270066

    申请日:2021-12-27

    IPC分类号: C12M1/42 C12M1/00 C12M1/34

    摘要: A flow electrotransfection device with a chamber for fluid to flow though, a liquid inlet channel for communicating with the chamber and a liquid outlet channel for communicating with the chamber, includes an electrode module for applying an electric field to fluid in the chamber, and further includes a fluid guiding structure for making flow velocities of fluid flowing through the central zone and the peripheral zone of the chamber tend to be consistent. The present disclosure solves the technical problem of relatively uneven the flow velocities of fluid at different zones in the flow electrotransfection chamber, improves the accuracy of the number of electric shock when the fluid flows though the chamber, thus improving the electrotransfection efficiency, and reduces the cell damage and even death from excessive times of electric shock, thereby improving cell viability.

    Reactor having dynamic sparger
    16.
    发明授权

    公开(公告)号:US11898134B2

    公开(公告)日:2024-02-13

    申请号:US18051295

    申请日:2022-10-31

    申请人: LanzaTech, Inc.

    IPC分类号: C12M1/00

    摘要: Systems and methods relating to dynamic spargers for generating fine bubbles within reactors such as biological and chemical reactors. A sparger system is positioned within a reactor and comprises a support plate, multiple annular shrouds engaged with the support plate, and spargers positioned within the annular shrouds defining a gap between an interior surface of the annular shroud and an exterior surface of the corresponding sparger. Liquid flows through the defined gap between an interior surface of the annular shroud and an exterior surface of the sparger. Acceleration of the liquid through the gap shears bubbles at the exterior surface of the sparger creating bubbles or fine bubbles.

    CULTURE VESSEL, AND METHOD OF USING CULTURE VESSEL

    公开(公告)号:US20240034973A1

    公开(公告)日:2024-02-01

    申请号:US18255545

    申请日:2021-12-01

    发明人: Makoto YAMANAKA

    IPC分类号: C12M1/00 C12M1/12 C12M1/34

    CPC分类号: C12M23/40 C12M25/16 C12M41/40

    摘要: A culture vessel has a culture space therein and may culture cells in the culture space. The culture vessel includes a main flow path extending within a plane, and a secondary flow path that is adjacent to the main flow path in a direction perpendicular to the plane, extends in a direction intersecting the main flow path when viewed from a direction perpendicular to the plane, and partially overlaps the main flow path. The secondary flow path has a groove that is open to and communicates with the main flow path in an area where the secondary flow path overlaps the main flow path, and the culture space is a space within the main flow path, the space being opposed to a contact surface where the secondary flow path is in contact with the main flow path in the area where the secondary flow path overlaps the main flow path.