公开(公告)号：US12053778B2

公开(公告)日：2024-08-06

申请号：US17529498

申请日：2021-11-18

Applicant: LUMICKS CA HOLDING B.V.

Inventor： Andrea Candelli ,  Felix Oswald ,  Gerrit Sitters

IPC: B01L3/00 ,  B01L7/00 ,  G01N15/1429 ,  G01N21/03 ,  G01N21/17 ,  G01N29/036 ,  G01N29/22 ,  G01N29/24 ,  G01N29/34 ,  G01N21/64

CPC classification number: B01L3/502761 ,  B01L3/50273 ,  B01L7/00 ,  G01N15/1429 ,  G01N21/0332 ,  G01N29/036 ,  G01N29/222 ,  G01N29/2437 ,  G01N29/2462 ,  G01N29/346 ,  G01N29/348 ,  B01L2200/0652 ,  B01L2300/0663 ,  B01L2300/163 ,  B01L2300/1822 ,  B01L2400/0436 ,  G01N2021/177 ,  G01N21/6458 ,  G01N2291/014 ,  G01N2291/02466 ,  G01N2291/02475 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0257 ,  G01N2291/02809 ,  G01N2291/0423 ,  G01N2291/0426

Abstract: A method of manipulating and/or investigating cellular bodies (9) is provided. The method comprises the steps of: providing a sample holder (3) comprising a holding space (5) for holding a fluid medium (11); providing a sample (7) comprising one or more cellular bodies (9) in a fluid medium (11) in the holding space (5); generating an acoustic wave in the holding space exerting a force (F) on the sample (7) in the holding space (5). The method further comprises providing the holding space (5) with a functionalised wall surface portion (17) to be contacted by the sample (7) and the sample (7) is in contact with the functionalised wall surface portion (17) during at least part of the step of application of the acoustic wave. A system and a sample holder (3) are also provided.

公开(公告)号：US20240253045A1

公开(公告)日：2024-08-01

申请号：US18440213

申请日：2024-02-13

Applicant: HIFIBIO SAS

Inventor： Annabelle Patricia Veronique Gérard ,  Vera Menrath

IPC: B01L3/00 ,  G01N33/543 ,  G01N33/569

CPC classification number: B01L3/502784 ,  G01N33/54366 ,  G01N33/569 ,  B01L2200/0652 ,  B01L2200/16 ,  B01L2400/02 ,  G01N2458/00

Abstract: The present invention concerns a method for capturing and barcoding nucleic acid from single cells, a plurality of microfluidic droplets and a method for preparing said plurality of microfluidic droplets.

公开(公告)号：US12044612B2

公开(公告)日：2024-07-23

申请号：US17712888

申请日：2022-04-04

Applicant: Steven H. Reichenbach

Inventor： Steven H. Reichenbach

IPC: B01L3/00 ,  B01D43/00 ,  G01N15/10 ,  G01N15/01

CPC classification number: G01N15/1023 ,  B01D43/00 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1028

Abstract: An apparatus and method to segregate particles suspended in a fluid with an obstacle field in the flow path of the fluid. The particles may be dispersed after an interaction with obstacles in the obstacle field. The obstacle-particle interactions may result in an asymmetrical particle shift or bump in which the particles are differentially dispersed relative to the obstacle and the fluid flow. Individual obstacles having properties that are asymmetrical are arranged creating asymmetrical gaps between adjacent pairs of obstacles to separate particles flowing through the device.

公开(公告)号：US12042792B2

公开(公告)日：2024-07-23

申请号：US16683917

申请日：2019-11-14

Applicant: Massachusetts Institute of Technology

Inventor： Jongyoon Han ,  Hyungkook Jeon

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L2200/0652 ,  B01L2300/0809 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/0487

Abstract: Described is a multi-dimensional double spiral (MDDS) microfluidic device comprising a first spiral microchannel and a second microchannel, wherein the wherein the first spiral microchannel and second spiral microchannel have different cross-sectional areas. Also described is a device comprising a multi-dimensional double spiral and system for recirculation. The invention also encompasses methods of separating particles from a sample fluid comprising a mixture of particles comprising the use of the multi-dimensional double spiral microfluidic device.

公开(公告)号：US20240238786A1

公开(公告)日：2024-07-18

申请号：US18563200

申请日：2022-05-20

Applicant: Verily Life Sciences LLC

Inventor： Saurabh Vyawahare ,  Andrew Homyk

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0652 ,  B01L2300/023 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/0415

Abstract: A droplet sorting device is described. The droplet sorting device includes a primary channel, a sorting channel, a sorting electrode, a ground film, and an insulating layer. The sorting channel may intersect the primary channel to define a sorting junction. The sorting electrode may be disposed in a first plane adjacent to the sorting junction. The ground film may be disposed in a second plane that is different from the first plane. The insulating layer may be disposed in a third plane that is located between the first and second planes.

公开(公告)号：US20240207849A1

公开(公告)日：2024-06-27

申请号：US18595902

申请日：2024-03-05

Applicant: RESTEK CORPORATION

Inventor： Tracey A. PETERS ,  German A. GOMEZ-RIOS ,  Thomas E. KANE

IPC: B01L3/00

CPC classification number: B01L3/502753 ,  B01L2200/0621 ,  B01L2200/0631 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/0864 ,  B01L2400/0487

Abstract: A solid phase microextraction device is disclosed, including a substrate having a planar surface and a sorbent layer disposed on the planar surface. The planar surface is defined by a base edge, a spray edge disposed distal across the substrate from the base edge, the spray edge including a tapering tip extending away from the base edge, a first lateral edge extending from the base edge to the tapering tip, and a second lateral edge extending from the base edge to the tapering tip, the second lateral edge being disposed distal across the substrate from the first lateral edge. The sorbent layer extends a sampling length from the spray edge toward the base edge and includes sorbent particles. A method for forming the solid phase microextraction device is disclosed, including applying the sorbent layer on the planar surface utilizing at least one of screen printing, stencil printing, or additive manufacturing.

公开(公告)号：US20240198340A1

公开(公告)日：2024-06-20

申请号：US18415200

申请日：2024-01-17

Applicant: Iowa State University Research Foundation, Inc.

Inventor： Robbyn K. Anand ,  Sungu Kim ,  Baskar Ganapathysubramanian

IPC: B01L3/00

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/047 ,  B01L2300/0645 ,  B01L2300/0864 ,  B01L2400/0421

Abstract: Microfluidic devices and methods that utilize ion concentration polarization within nanoliter scale droplets for concentration enrichment, separation, and substitution of charges species are disclosed. Such devices and methods can be used for separation of multiple species by mobility of each species and for the alteration and manipulation of the droplet composition by ion exchange.

公开(公告)号：US20240192109A1

公开(公告)日：2024-06-13

申请号：US18550871

申请日：2022-03-18

Applicant: NANYANG TECHNOLOGICAL UNIVERSITY

Inventor： Han Wei HOU ,  Chayakorn PETCHAKUP ,  King Ho Holden LI

IPC: G01N15/02 ,  B01L3/00 ,  G01N15/10 ,  G01N15/1031

CPC classification number: G01N15/0266 ,  B01L3/502761 ,  B01L3/502776 ,  G01N15/1023 ,  G01N15/1031 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2400/0487 ,  G01N2015/1006 ,  G01N2015/1028 ,  G01N2015/1029

Abstract: The present invention relates to a microfluidic impedance cytometer comprising an integrated biosensor and use of the device thereof for label-free activation profiling of cells in a sample, such as myelocytes and/or lymphocytes and neutrophils. The microfluidic impedance cytometer may comprise a spiral-shaped flow channel, coplanar electrodes for generating an electric field across the channel, a first sample inlet and a second sheath fluid inlet; wherein the sample inlet is a stepped sample inlet in that the height of the sample inlet is lower than the height of the sheath fluid inlet and the flow channel.

公开(公告)号：US20240181454A1

公开(公告)日：2024-06-06

申请号：US18062311

申请日：2022-12-06

Applicant: ASTRIN BIOSCIENCES, INC.

Inventor： Jiarong HONG ,  Mahdi AHMADI ,  Kaylee SMITH ,  Jayant PARTHASARATHY

IPC: B01L3/00 ,  G01N35/00

CPC classification number: B01L3/502753 ,  G01N35/0099 ,  B01L2200/04 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2400/0409

Abstract: The present embodiments relate to an apheretic system incorporating one or more centrifuge devices. A biological fluid containing cells (or another fluid) can be obtained and directed to a centrifuge device to separate particles by a particle size and/or a flow rate. The centrifuge device can include a center-axis centrifuge, an off-center centrifuge, and/or a hydro-cyclone centrifuge device. The separated particles can be directed/drawn into either an outlet to a return or into a collection container for further testing.

公开(公告)号：US11998913B2

公开(公告)日：2024-06-04

申请号：US18120537

申请日：2023-03-13

Applicant: Cellular Highways Ltd.

Inventor： Mikhail Bashtanov ,  Richard Gold ,  Calum Hayes ,  Fred Hussain ,  Robyn Pritchard ,  Salman Samson Rogers ,  Nuno Varelas ,  Alexander Zhukov

IPC: B01L3/00 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/149

CPC classification number: B01L3/502753 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502776 ,  G01N15/1434 ,  B01L2200/0626 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/1805 ,  B01L2400/0442 ,  B01L2400/0487 ,  B01L2400/06 ,  G01N2015/1413 ,  G01N15/149

Abstract: A single junction sorter for a microfluidic particle sorter, the single-junction sorter comprising: an input channel, configured to receive a fluid containing particles; an output sort channel and an output waste channel, each connected to the input channel for receiving the fluid therefrom; a bubble generator, operable to selectively displace the fluid around a particle to be sorted and thereby to create a transient flow of the fluid in the input channel; and a vortex element, configured to cause a vortex in the transient flow in order to direct the particle to be sorted into the output sort channel.