公开(公告)号：US20190078054A1

公开(公告)日：2019-03-14

申请号：US16138724

申请日：2018-09-21

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Nicholas E. Propson ,  Michael P. Schwartz ,  Zhonggang Hou ,  Gene I. Uenishi ,  Igor I. Slukvin ,  William L. Murphy ,  Jue Zhang

IPC: C12N5/079 ,  C12N5/0786 ,  G01N33/50 ,  C12N5/071 ,  C12N5/0789 ,  C12N5/00

Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

公开(公告)号：US20190010461A1

公开(公告)日：2019-01-10

申请号：US16029068

申请日：2018-07-06

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Jue Zhang

IPC: C12N5/077 ,  A61K31/4375

CPC classification number: C12N5/0661 ,  A61K31/4375 ,  C12N5/0606 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/42 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/04 ,  C12N2510/00 ,  C12N2533/90

Abstract: Methods of using a small molecule MYH11 agonist to inhibit intimal hyperplasia and to maintain a contractile phenotype in vitro and in vivo are described. Also described herein are methods for generating human contractile smooth muscle cells from human pluripotent stem cells under defined conditions in the presence of the small molecule MYH11 agonist.

公开(公告)号：US09771556B2

公开(公告)日：2017-09-26

申请号：US14818779

申请日：2015-08-05

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Zhonggang Hou ,  Guokai Chen

IPC: C12N5/02 ,  C12N5/0735 ,  C12N5/074 ,  C07K14/78

CPC classification number: C12N5/0606 ,  C07K14/78 ,  C12N5/0696 ,  C12N2500/02 ,  C12N2500/05 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/727 ,  C12N2533/50 ,  C12N2533/52

Abstract: Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented. Also provided herein are defined culture systems for maintaining human pluripotent stem cells in a substantially undifferentiated state, where the defined culture system comprises human pluripotent stem cells, a defined culture medium, and at least one polypeptide selected from the group consisting of residues 43 to 378 of SEQ ID NO:1 and residues 45 to 378 of SEQ ID NO:1, and where the defined culture medium comprises insulin, selenium, transferrin, L-ascorbic acid, FGF2, DMEM/F12, NaHCO3, and one of TGFβ and NODAL.

公开(公告)号：US20130236962A1

公开(公告)日：2013-09-12

申请号：US13857408

申请日：2013-04-05

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： James A. Thomson ,  Tenneille Ludwig

IPC: C12N5/0735 ,  C12N5/071

CPC classification number: C12N5/0606 ,  C12N5/0696 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/845

Abstract: Previous methods for culturing primate pluripotent stem cells have required either fibroblast feeder cells or a medium which was exposed to fibroblast feeder cells to maintain the stem cells in an undifferentiated state. It has now been found that high levels of fibroblast growth factor in a medium together with at least one of gamma aminobutyric acid, pipecolic acid, and lithium, enables pluripotent stem cells to remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium. Without beta-mercaptoethanol, the medium improves cloning efficiency. Also, a matrix of human proteins can be used to culture the undifferentiated cells without exposing the cells to animal products. Further disclosed are new primate pluripotent cell lines made using the defined culture conditions, including the medium and the matrix. Such new cell lines will have never been exposed to animal cells, animal products, feeder cells or conditioned medium.

Abstract translation: 培养灵长类多能干细胞的以前的方法需要成纤维细胞饲养细胞或暴露于成纤维细胞饲养细胞的培养基以维持干细胞处于未分化状态。 现在已经发现，培养基中高水平的成纤维细胞生长因子与γ-氨基丁酸，哌啶酸和锂中的至少一种一起使多能干细胞通过多次传代无限期地保持未分化，即使没有饲养细胞或条件培养基 。 没有β-巯基乙醇，培养基提高了克隆效率。 此外，可以使用人类蛋白质的基质来培养未分化细胞而不将细胞暴露于动物产品。 进一步公开的是使用定义的培养条件制备的新的灵长类动物多能细胞系，包括培养基和基质。 这样的新细胞系从未暴露于动物细胞，动物产品，饲养细胞或条件培养基。

公开(公告)号：US11898162B2

公开(公告)日：2024-02-13

申请号：US16818958

申请日：2020-03-13

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Junying Yu

IPC: C12N5/00 ,  C12N5/074 ,  C12N5/0735

CPC classification number: C12N5/0607 ,  C12N5/0696 ,  C12N5/0606 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2502/99 ,  C12N2510/00 ,  C12N2799/027

Abstract: The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.

公开(公告)号：US20230139291A1

公开(公告)日：2023-05-04

申请号：US18146998

申请日：2022-12-27

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： James A. Thomson ,  Srikumar Sengupta

IPC: C12N5/071 ,  A61K35/407

Abstract: Methods for derivation, culture, and maturation of small hepatic progenitor cells are described.

公开(公告)号：US20220016178A1

公开(公告)日：2022-01-20

申请号：US17443786

申请日：2021-07-27

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Srikumar Sengupta

IPC: A61K35/407 ,  C12N5/071 ,  C12Q1/02 ,  G01N33/50

Abstract: This disclosure relates generally to new methods of maintaining the expression of hepatic genes in human hepatocytes and method for maintaining the functional hepatic enzyme activity of primary hepatocytes in culture. The disclosure also encompasses new methods of deriving a population of pure hepatocytes without selecting or sorting the cells from the cultured pluripotent cells.

公开(公告)号：US10081792B2

公开(公告)日：2018-09-25

申请号：US14986224

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Nicholas E. Propson ,  Michael P. Schwartz ,  Zhonggang Hou ,  Gene I. Uenishi ,  Igor I. Slukvin ,  William L. Murphy ,  Jue Zhang

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C12N5/079 ,  C12N5/0789 ,  G01N33/50 ,  C12N5/0786

CPC classification number: C12N5/0622 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0645 ,  C12N5/0647 ,  C12N5/0697 ,  C12N2500/25 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/22 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2513/00 ,  G01N33/5014 ,  G01N33/5058

Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

公开(公告)号：US20180015126A1

公开(公告)日：2018-01-18

申请号：US15650595

申请日：2017-07-14

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Srikumar Sengupta

IPC: A61K35/407 ,  C12N5/071 ,  C12Q1/02 ,  C12Q1/00 ,  C12N5/00

CPC classification number: A61K35/407 ,  C12N5/00 ,  C12N5/067 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/237 ,  C12N2501/39 ,  C12N2501/42 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2503/02 ,  C12N2506/02 ,  C12Q1/00 ,  C12Q1/02 ,  G01N33/50

Abstract: This disclosure relates generally to new methods of maintaining the expression of hepatic genes in human hepatocytes and method for maintaining the functional hepatic enzyme activity of primary hepatocytes in culture. The disclosure also encompasses new methods of deriving a population of pure hepatocytes without selecting or sorting the cells from the cultured pluripotent cells.

公开(公告)号：US09340771B2

公开(公告)日：2016-05-17

申请号：US14703407

申请日：2015-05-04

Applicant: Wisconsin Alumni Research Foundation

Inventor： Guokai Chen ,  James A. Thomson

IPC: C12N5/00 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0606 ,  C12N5/0696 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/91 ,  C12N2506/02 ,  C12N2506/1307

Abstract: Methods are provided that exploit thermostable FGF-1 proteins for support of human pluripotent stem cell cultures. Also provided are compositions containing thermostable FGF-1 for culturing of human pluripotent stem cells.