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公开(公告)号：US11116799B2
公开(公告)日：2021-09-14
申请号：US15650595
申请日：2017-07-14
Applicant: Wisconsin Alumni Research Foundation
Inventor： James A. Thomson , Srikumar Sengupta
Abstract: This disclosure relates generally to new methods of maintaining the expression of hepatic genes in human hepatocytes and method for maintaining the functional hepatic enzyme activity of primary hepatocytes in culture. The disclosure also encompasses new methods of deriving a population of pure hepatocytes without selecting or sorting the cells from the cultured pluripotent cells.
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公开(公告)号：US20200071664A1
公开(公告)日：2020-03-05
申请号：US16557404
申请日：2019-08-30
Applicant: Wisconsin Alumni Research Foundation
Inventor： James A. Thomson , Srikumar Sengupta
IPC: C12N5/071 , A61K35/407
Abstract: Methods for derivation, culture, and maturation of small hepatic progenitor cells are described.
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公开(公告)号：US20230113074A1
公开(公告)日：2023-04-13
申请号：US17963806
申请日：2022-10-11
Applicant: Wisconsin Alumni Research Foundation
Inventor： Srikumar Sengupta , James Thomson
IPC: C12N5/0735
Abstract: This disclosure relates generally to methods for generating small hepatocyte progenitor cells (SHPCs) and hematopoietic progenitor cells (HPCs) from human embryonic stem cells, and hematopoietic progenitor cells from primary human endothelial cells and cell lines populations of small hepatocyte progenitor cells and hematopoietic progenitor cells, and uses thereof.
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公开(公告)号：US11566229B2
公开(公告)日：2023-01-31
申请号：US16557404
申请日：2019-08-30
Applicant: Wisconsin Alumni Research Foundation
Inventor： James A. Thomson , Srikumar Sengupta
IPC: C12N5/071 , A61K35/407
Abstract: Methods for derivation, culture, and maturation of small hepatic progenitor cells are described.
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公开(公告)号：US09512428B2
公开(公告)日：2016-12-06
申请号：US14822099
申请日：2015-08-10
Applicant: Wisconsin Alumni Research Foundation
Inventor： Srikumar Sengupta , James Thomson
IPC: C12N15/11 , C12N15/113 , C12N5/071 , A61K35/407 , A61K35/545 , G01N33/50 , A61K38/38 , A61K38/44 , A61K38/45 , A61K38/57
CPC classification number: C12N15/113 , A61K35/407 , A61K35/545 , A61K38/38 , A61K38/44 , A61K38/45 , A61K38/57 , C12N5/067 , C12N5/0672 , C12N15/111 , C12N2310/141 , C12N2320/30 , C12N2501/65 , C12N2510/00 , C12Y114/13017 , C12Y114/14001 , C12Y206/01005 , G01N33/5014
Abstract: A method for producing mature hepatocytes having functional hepatic enzyme activity from human pluripotent cells is disclosed. The method includes the step of transferring an external vector comprising the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-122, the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-let-7c, a microRNA having the seed sequence of the microRNA miR-122, a microRNA having the seed sequence of the microRNA miR-let-7c, or a combination thereof into one or more fetal hepatocytes. The resulting cells differentiate into mature hepatocytes that exhibit functional hepatic enzyme activity, and can be used in drug metabolism and toxicity testing, in the study of viruses that target hepatic tissue, and as therapeutics.A related method of maintaining the functional hepatic enzyme activity of primary hepatocytes over time is also disclosed. The method includes the step of transferring an external vector comprising the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-122 into one or more cultured primary hepatocytes.
Abstract translation: 还公开了维持原代肝细胞功能性肝酶活性的相关方法。 该方法包括将包含编码具有微小RNA miR-122的种子序列的微小RNA的DNA序列的外部载体转移到一个或多个培养的原代肝细胞中的步骤。
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Patent Agency Ranking
6.发明申请公开(公告)号：US20180230546A1
公开(公告)日：2018-08-16
申请号：US15880007
申请日：2018-01-25
Applicant: Wisconsin Alumni Research Foundation
Inventor： Paul Gerald Ahlquist , Srikumar Sengupta , Johan Arie den Boon , Bill Sugden , Michael Abbott Newton
IPC: C12Q1/6886 , C12Q1/6809 , C12N15/113
CPC classification number: C12Q1/6886 , C12N15/113 , C12N2310/14 , C12N2320/11 , C12N2330/10 , C12Q1/6809 , C12Q2600/106 , C12Q2600/158 , C12Q2600/178 , C12Q2537/143 , C12Q2525/207
Abstract: This invention provides methods for amplifying, detecting, measuring, and identifying miRNAs from biological samples, particularly limited amounts of a biological sample. miRNAs that are differentially expressed in tumor samples and normal tissues are useful as cancer biomarkers for cancer diagnostics.
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7.发明申请公开(公告)号：US20160319364A1
公开(公告)日：2016-11-03
申请号：US15013189
申请日：2016-07-19
Applicant: Wisconsin Alumni Research Foundation
Inventor： Paul Gerald Ahlquist , Srikumar Sengupta , Johan Arie den Boon , Bill Sugden , Michael Abbott Newton
IPC: C12Q1/68
CPC classification number: C12Q1/6886 , C12N15/113 , C12N2310/14 , C12N2320/11 , C12N2330/10 , C12Q1/6809 , C12Q2600/106 , C12Q2600/158 , C12Q2600/178 , C12Q2537/143 , C12Q2525/207
Abstract: This invention provides methods for amplifying, detecting, measuring, and identifying miRNAs from biological samples, particularly limited amounts of a biological sample. miRNAs that are differentially expressed in tumor samples and normal tissues are useful as cancer biomarkers for cancer diagnostics.
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公开(公告)号：US20160032287A1
公开(公告)日：2016-02-04
申请号：US14822099
申请日：2015-08-10
Applicant: Wisconsin Alumni Research Foundation
Inventor： Srikumar Sengupta , James Thomson
IPC: C12N15/113 , C12N5/071
CPC classification number: C12N15/113 , A61K35/407 , A61K35/545 , A61K38/38 , A61K38/44 , A61K38/45 , A61K38/57 , C12N5/067 , C12N5/0672 , C12N15/111 , C12N2310/141 , C12N2320/30 , C12N2501/65 , C12N2510/00 , C12Y114/13017 , C12Y114/14001 , C12Y206/01005 , G01N33/5014
Abstract: A method for producing mature hepatocytes having functional hepatic enzyme activity from human pluripotent cells is disclosed. The method includes the step of transferring an external vector comprising the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-122, the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-let-7c, a microRNA having the seed sequence of the microRNA miR-122, a microRNA having the seed sequence of the microRNA miR-let-7c, or a combination thereof into one or more fetal hepatocytes. The resulting cells differentiate into mature hepatocytes that exhibit functional hepatic enzyme activity, and can be used in drug metabolism and toxicity testing, in the study of viruses that target hepatic tissue, and as therapeutics.A related method of maintaining the functional hepatic enzyme activity of primary hepatocytes over time is also disclosed. The method includes the step of transferring an external vector comprising the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-122 into one or more cultured primary hepatocytes.
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公开(公告)号：US09127253B2
公开(公告)日：2015-09-08
申请号：US13772802
申请日：2013-02-21
Applicant: Wisconsin Alumni Research Foundation
Inventor： Srikumar Sengupta , James Thomson
IPC: C12N15/00 , C12N5/071 , A61K35/407 , C12N15/11 , C12N15/113 , A61K35/545 , G01N33/50 , A61K38/38 , A61K38/44 , A61K38/45 , A61K38/57
CPC classification number: C12N15/113 , A61K35/407 , A61K35/545 , A61K38/38 , A61K38/44 , A61K38/45 , A61K38/57 , C12N5/067 , C12N5/0672 , C12N15/111 , C12N2310/141 , C12N2320/30 , C12N2501/65 , C12N2510/00 , C12Y114/13017 , C12Y114/14001 , C12Y206/01005 , G01N33/5014
Abstract: A method for producing mature hepatocytes having functional hepatic enzyme activity from human pluripotent cells is disclosed. The method includes the step of transferring an external vector that includes a microRNA having the seed sequence of the microRNA miR-122, a DNA sequence coding for such a microRNA, a microRNA having the seed sequence of the microRNA miR-let-7c, a DNA sequence coding for such a microRNA, or a combination these, into one or more fetal hepatocytes. The resulting cells differentiate into mature hepatocytes that exhibit functional hepatic enzyme activity, and that can be used in drug metabolism and toxicity testing, in the study of viruses that target hepatic tissue, and as therapeutics.
Abstract translation: 公开了一种从人多能细胞产生具有功能性肝酶活性的成熟肝细胞的方法。 该方法包括转移包含具有微小RNA miR-122的种子序列的微小RNA的外部载体的步骤，编码这种微小RNA的DNA序列，具有微小RNA miR-let-7c的种子序列的微小RNA， 编码这种微小RNA的DNA序列，或这些的组合，形成一个或多个胎儿肝细胞。 所产生的细胞分化成具有功能性肝酶活性的成熟肝细胞，并且可用于药物代谢和毒性试验，用于靶向肝组织的病毒的研究以及作为治疗剂。
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公开(公告)号：US20230139291A1
公开(公告)日：2023-05-04
申请号：US18146998
申请日：2022-12-27
Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventor： James A. Thomson , Srikumar Sengupta
IPC: C12N5/071 , A61K35/407
Abstract: Methods for derivation, culture, and maturation of small hepatic progenitor cells are described.
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