公开(公告)号：US20200239840A1

公开(公告)日：2020-07-30

申请号：US16818958

申请日：2020-03-13

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Junying Yu

IPC: C12N5/074

Abstract: The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.

公开(公告)号：US10724002B2

公开(公告)日：2020-07-28

申请号：US15946101

申请日：2018-04-05

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC: C12N5/078

Abstract: Methods and compositions of erythroid cells that produce adult β-hemoglobin, generated by culturing CD31+, CD31+/CD34+ or CD34+ cells from embryonic stem cells under serum-free culture conditions.

公开(公告)号：US09499786B2

公开(公告)日：2016-11-22

申请号：US13766100

申请日：2013-02-13

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Junying Yu

IPC: C12N15/00 ,  C12N5/00 ,  C12N5/074 ,  C12N5/0735

CPC classification number: C12N5/0607 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2502/99 ,  C12N2510/00 ,  C12N2799/027

Abstract: The present invention relates to methods for reprogramming a somatic cell to pluripotency by administering into the somatic cell at least one or a plurality of potency-determining factors. The invention also relates to pluripotent cell populations obtained using a reprogramming method.

Abstract translation: 本发明涉及通过向体细胞施用至少一种或多种效力决定因素来将体细胞重编程为多能性的方法。 本发明还涉及使用重编程方法获得的多能细胞群体。

公开(公告)号：US20160186135A1

公开(公告)日：2016-06-30

申请号：US14986382

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Jue Zhang

IPC: C12N5/071

CPC classification number: C12N5/069 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2506/45

Abstract: The present invention relates to chemically defined and xenogeneic material-free methods for deriving endothelial cells from human pluripotent stem cells. In particular, the present invention provides highly efficient and reproducible methods of obtaining human endothelial cells from human pluripotent stem cells, where endothelial cells derived from the methods provided herein are suitable for clinically relevant therapeutic applications.

Abstract translation: 本发明涉及用于从人多能干细胞衍生内皮细胞的化学定义和异种材料无材料的方法。 特别地，本发明提供从人多能干细胞获得人内皮细胞的高效和可重复的方法，其中源自本文提供的方法的内皮细胞适合临床相关的治疗应用。

公开(公告)号：US20150267166A1

公开(公告)日：2015-09-24

申请号：US14703407

申请日：2015-05-04

Applicant: Wisconsin Alumni Research Foundation

Inventor： Guokai Chen ,  James A. Thomson

IPC: C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0606 ,  C12N5/0696 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/91 ,  C12N2506/02 ,  C12N2506/1307

Abstract: Methods are provided that exploit thermostable FGF-1 proteins for support of human pluripotent stem cell cultures. Also provided are compositions containing thermostable FGF-1 for culturing of human pluripotent stem cells.

Abstract translation: 提供了利用热稳定性FGF-1蛋白质来支持人多能干细胞培养物的方法。 还提供了含有用于培养人多能干细胞的耐热FGF-1的组合物。

公开(公告)号：US08785189B2

公开(公告)日：2014-07-22

申请号：US13859228

申请日：2013-04-09

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC: C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  C12N5/0784

CPC classification number: C12N5/0639 ,  C12N5/064 ,  C12N2506/02

Abstract: This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.

Abstract translation: 本发明涉及来自人胚胎干（ES）细胞的树突状细胞的培养。 首先通过与基质细胞共培养将人类ES细胞培养成造血细胞。 然后将分化成造血谱系的细胞与GM-CSF一起培养以产生骨髓前体细胞的培养物。 用细胞因子GM-CSF和IL-4培养骨髓前体细胞会导致产生功能性树突状细胞。 树突细胞具有独特的表型，如细胞表面标志物的组合所示。

公开(公告)号：US20220017873A1

公开(公告)日：2022-01-20

申请号：US17373523

申请日：2021-07-12

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  William L. Murphy ,  Charles D. Page ,  Michael P. Schwartz ,  Zhonggang Hou

IPC: C12N5/071 ,  C12N5/079 ,  C12N5/0793 ,  G01N33/50

Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.

公开(公告)号：US11124765B2

公开(公告)日：2021-09-21

申请号：US16138724

申请日：2018-09-21

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Nicholas E. Propson ,  Michael P. Schwartz ,  Zhonggang Hou ,  Gene I. Uenishi ,  Igor I. Slukvin ,  William L. Murphy ,  Jue Zhang

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C12N5/079 ,  C12N5/0789 ,  G01N33/50 ,  C12N5/0786 ,  C12N5/0735

Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

公开(公告)号：US11116799B2

公开(公告)日：2021-09-14

申请号：US15650595

申请日：2017-07-14

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Srikumar Sengupta

IPC: A61K35/407 ,  C12N5/071 ,  C12Q1/02 ,  G01N33/50 ,  C12N5/00 ,  C12Q1/00

Abstract: This disclosure relates generally to new methods of maintaining the expression of hepatic genes in human hepatocytes and method for maintaining the functional hepatic enzyme activity of primary hepatocytes in culture. The disclosure also encompasses new methods of deriving a population of pure hepatocytes without selecting or sorting the cells from the cultured pluripotent cells.

公开(公告)号：US20200071664A1

公开(公告)日：2020-03-05

申请号：US16557404

申请日：2019-08-30

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Srikumar Sengupta

IPC: C12N5/071 ,  A61K35/407

Abstract: Methods for derivation, culture, and maturation of small hepatic progenitor cells are described.