公开(公告)号：US11060066B2

公开(公告)日：2021-07-13

申请号：US14986363

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  William L. Murphy ,  Charles D. Page ,  Michael P. Schwartz ,  Zhonggang Hou

IPC: C12N5/071 ,  C12N5/079 ,  C12N5/0793 ,  G01N33/50 ,  C12Q1/6883

Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.

公开(公告)号：US20160186146A1

公开(公告)日：2016-06-30

申请号：US14986363

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  William L. Murphy ,  Charles D. Page ,  Michael P. Schwartz ,  Zhonggang Hou

IPC: C12N5/071

CPC classification number: C12N5/0697 ,  C12N5/0619 ,  C12N5/0622 ,  C12N2502/086 ,  C12N2502/1358 ,  C12N2502/28 ,  C12N2503/02 ,  C12N2503/04 ,  C12N2506/02 ,  C12N2533/30 ,  C12N2533/90 ,  C12Q1/6883 ,  C12Q2600/142 ,  C12Q2600/158 ,  G01N33/5058

Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.

Abstract translation: 本发明涉及三维（3D）组织构建体和使用这种3D组织构建体来筛选神经毒性试剂的方法。 特别地，本文提供了生产和使用包含生理相关的人细胞的复杂的高度均匀的人组织模型的方法，其中组织模型具有用于定量高通量筛选应用所需的样品均匀度和再现性的程度。

公开(公告)号：US20220017873A1

公开(公告)日：2022-01-20

申请号：US17373523

申请日：2021-07-12

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  William L. Murphy ,  Charles D. Page ,  Michael P. Schwartz ,  Zhonggang Hou

IPC: C12N5/071 ,  C12N5/079 ,  C12N5/0793 ,  G01N33/50

Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.