公开(公告)号：US10081792B2

公开(公告)日：2018-09-25

申请号：US14986224

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Nicholas E. Propson ,  Michael P. Schwartz ,  Zhonggang Hou ,  Gene I. Uenishi ,  Igor I. Slukvin ,  William L. Murphy ,  Jue Zhang

IPC: C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C12N5/079 ,  C12N5/0789 ,  G01N33/50 ,  C12N5/0786

CPC classification number: C12N5/0622 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0645 ,  C12N5/0647 ,  C12N5/0697 ,  C12N2500/25 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/22 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2513/00 ,  G01N33/5014 ,  G01N33/5058

Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

公开(公告)号：US20220033773A1

公开(公告)日：2022-02-03

申请号：US17405579

申请日：2021-08-18

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Nicholas E. Propson ,  Michael P. Schwartz ,  Zhonggang Hou ,  Gene I. Uenishi ,  Igor I Slukvin ,  William L. Murphy ,  Jue Zhang

IPC: C12N5/079 ,  C12N5/0789 ,  C12N5/071 ,  G01N33/50 ,  C12N5/0786

Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

公开(公告)号：US11060066B2

公开(公告)日：2021-07-13

申请号：US14986363

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  William L. Murphy ,  Charles D. Page ,  Michael P. Schwartz ,  Zhonggang Hou

IPC: C12N5/071 ,  C12N5/079 ,  C12N5/0793 ,  G01N33/50 ,  C12Q1/6883

Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.

公开(公告)号：US20160186146A1

公开(公告)日：2016-06-30

申请号：US14986363

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  William L. Murphy ,  Charles D. Page ,  Michael P. Schwartz ,  Zhonggang Hou

IPC: C12N5/071

CPC classification number: C12N5/0697 ,  C12N5/0619 ,  C12N5/0622 ,  C12N2502/086 ,  C12N2502/1358 ,  C12N2502/28 ,  C12N2503/02 ,  C12N2503/04 ,  C12N2506/02 ,  C12N2533/30 ,  C12N2533/90 ,  C12Q1/6883 ,  C12Q2600/142 ,  C12Q2600/158 ,  G01N33/5058

Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.

Abstract translation: 本发明涉及三维（3D）组织构建体和使用这种3D组织构建体来筛选神经毒性试剂的方法。 特别地，本文提供了生产和使用包含生理相关的人细胞的复杂的高度均匀的人组织模型的方法，其中组织模型具有用于定量高通量筛选应用所需的样品均匀度和再现性的程度。

公开(公告)号：US20160186137A1

公开(公告)日：2016-06-30

申请号：US14986224

申请日：2015-12-31

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Nicholas E. Propson ,  Michael P. Schwartz ,  Zhonggang Hou ,  Gene I. Uenishi ,  Igor I. Slukvin ,  William L. Murphy ,  Jue Zhang

IPC: C12N5/079 ,  C12N5/071 ,  G01N33/50 ,  C12N5/0789

CPC classification number: C12N5/0622 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0645 ,  C12N5/0647 ,  C12N5/0697 ,  C12N2500/25 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/22 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2513/00 ,  G01N33/5014 ,  G01N33/5058

Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.

Abstract translation: 本发明涉及从人多能干细胞衍生人类造血祖细胞，原始巨噬细胞和小胶质细胞的方法。 特别地，本文提供的是从人多能干细胞获得人原始巨噬细胞和小胶质细胞的高效和可重复的方法，其中原始巨噬细胞和小胶质细胞可适用于临床相关的治疗应用。

公开(公告)号：US10195313B2

公开(公告)日：2019-02-05

申请号：US14339938

申请日：2014-07-24

Applicant: Wisconsin Alumni Research Foundation

Inventor： William L. Murphy ,  Ngoc Nhi Thi Le ,  Stefan Zorn ,  Michael P. Schwartz ,  Eric Huy Dang Nguyen

IPC: G01N33/483 ,  A61L31/14 ,  C12N5/0735 ,  G01N33/50 ,  A61K47/42 ,  A61L29/16 ,  B01J19/00 ,  G01N21/64

Abstract: Patterned hydrogel arrays and methods of preparing patterned hydrogel arrays are disclosed. Advantageously, the methods used to prepare the patterned hydrogel arrays allow for controlling individual hydrogel spot conditions such as hydrogel spot modulus, hydrogel spot ligand identity and hydrogel spot ligand density, which allows for preparing a wide range of hydrogel spots in a single array format. Patterned hydrogel arrays can also be formed to include hydrogel-free pools surrounded by hydrogel. Additionally, the patterned hydrogel arrays of the present disclosure support the culture of a range of cell types. The patterned hydrogel arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.

公开(公告)号：US09688957B2

公开(公告)日：2017-06-27

申请号：US14684062

申请日：2015-04-10

Applicant: Wisconsin Alumni Research Foundation

Inventor： William L. Murphy ,  Ngoc Nhi Le ,  Michael P. Schwartz ,  Eric Huy Dang Nguyen ,  Stefan Zorn ,  Hamisha Ardalani ,  Matthew Zanotelli ,  Matthew Brian Parlato ,  David Gregory Belair ,  William T. Daly

IPC: C12N5/00 ,  C12N5/0735 ,  G01N33/483 ,  B01J19/00 ,  G01N21/64

CPC classification number: A61L31/145 ,  A61K47/42 ,  A61L29/16 ,  A61L2300/80 ,  B01J19/0046 ,  B01J2219/00596 ,  C12N5/0606 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2537/10 ,  G01N21/6452 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2610/00

Abstract: Hydrogel Compositions and methods of using hydrogel compositions are disclosed. Advantageously, the hydrogel compositions offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation. In particularly suitable embodiments, the hydrogel compositions of the present disclosure may be used to promote tubulogenesis of endothelial cells.

公开(公告)号：US20150293073A1

公开(公告)日：2015-10-15

申请号：US14339938

申请日：2014-07-24

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： William L. Murphy ,  Ngoc Nhi Thi Le ,  Stefan Zorn ,  Michael P. Schwartz ,  Eric Huy Dang Nguyen

IPC: G01N33/483 ,  B01J19/00

CPC classification number: A61L31/145 ,  A61K47/42 ,  A61L29/16 ,  A61L2300/80 ,  B01J19/0046 ,  B01J2219/00596 ,  C12N5/0606 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2537/10 ,  G01N21/6452 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2610/00

Abstract: Patterned hydrogel arrays and methods of preparing patterned hydrogel arrays are disclosed. Advantageously, the methods used to prepare the patterned hydrogel arrays allow for controlling individual hydrogel spot conditions such as hydrogel spot modulus, hydrogel spot ligand identity and hydrogel spot ligand density, which allows for preparing a wide range of hydrogel spots in a single array format. Patterned hydrogel arrays can also be formed to include hydrogel-free pools surrounded by hydrogel. Additionally, the patterned hydrogel arrays of the present disclosure support the culture of a range of cell types. The patterned hydrogel arrays offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation.

Abstract translation: 公开了图案化水凝胶阵列和制备图案化水凝胶阵列的方法。 有利地，用于制备图案化水凝胶阵列的方法允许控制各种水凝胶斑点条件，例如水凝胶光点模量，水凝胶点配体同一性和水凝胶点配体密度，这允许以单阵列形式制备宽范围的水凝胶点。 图案化的水凝胶阵列也可以形成为包括水凝胶包围的无水凝胶池。 此外，本公开的图案化水凝胶阵列支持一系列细胞类型的培养。 图案化水凝胶阵列提供快速筛选底物组分以影响细胞附着，扩散，增殖，迁移和分化的能力。

公开(公告)号：US20150291929A1

公开(公告)日：2015-10-15

申请号：US14684062

申请日：2015-04-10

Applicant: Wisconsin Alumni Research Foundation

Inventor： William L. Murphy ,  Ngoc Nhi Le ,  Michael P. Schwartz ,  Eric Huy Dang Nguyen ,  Stefan Zorn ,  Hamisha Ardalani ,  Matthew Zanotelli ,  Matthew Brian Parlato ,  David Gregory Belair ,  William T. Daly

IPC: C12N5/00

CPC classification number: A61L31/145 ,  A61K47/42 ,  A61L29/16 ,  A61L2300/80 ,  B01J19/0046 ,  B01J2219/00596 ,  C12N5/0606 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2537/10 ,  G01N21/6452 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2610/00

Abstract: Hydrogel Compositions and methods of using hydrogel compositions are disclosed. Advantageously, the hydrogel compositions offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation. In particularly suitable embodiments, the hydrogel compositions of the present disclosure may be used to promote tubulogenesis of endothelial cells.

Abstract translation: 公开了水凝胶组合物和使用水凝胶组合物的方法。 有利地，水凝胶组合物提供快速筛选底物组分以影响细胞附着，扩散，增殖，迁移和分化的能力。 在特别合适的实施方案中，本公开的水凝胶组合物可用于促进内皮细胞的细胞生成。

公开(公告)号：US20220017873A1

公开(公告)日：2022-01-20

申请号：US17373523

申请日：2021-07-12

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  William L. Murphy ,  Charles D. Page ,  Michael P. Schwartz ,  Zhonggang Hou

IPC: C12N5/071 ,  C12N5/079 ,  C12N5/0793 ,  G01N33/50

Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.