公开(公告)号：US09688957B2

公开(公告)日：2017-06-27

申请号：US14684062

申请日：2015-04-10

Applicant: Wisconsin Alumni Research Foundation

Inventor： William L. Murphy ,  Ngoc Nhi Le ,  Michael P. Schwartz ,  Eric Huy Dang Nguyen ,  Stefan Zorn ,  Hamisha Ardalani ,  Matthew Zanotelli ,  Matthew Brian Parlato ,  David Gregory Belair ,  William T. Daly

IPC: C12N5/00 ,  C12N5/0735 ,  G01N33/483 ,  B01J19/00 ,  G01N21/64

CPC classification number: A61L31/145 ,  A61K47/42 ,  A61L29/16 ,  A61L2300/80 ,  B01J19/0046 ,  B01J2219/00596 ,  C12N5/0606 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2537/10 ,  G01N21/6452 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2610/00

Abstract: Hydrogel Compositions and methods of using hydrogel compositions are disclosed. Advantageously, the hydrogel compositions offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation. In particularly suitable embodiments, the hydrogel compositions of the present disclosure may be used to promote tubulogenesis of endothelial cells.

公开(公告)号：US20150291929A1

公开(公告)日：2015-10-15

申请号：US14684062

申请日：2015-04-10

Applicant: Wisconsin Alumni Research Foundation

Inventor： William L. Murphy ,  Ngoc Nhi Le ,  Michael P. Schwartz ,  Eric Huy Dang Nguyen ,  Stefan Zorn ,  Hamisha Ardalani ,  Matthew Zanotelli ,  Matthew Brian Parlato ,  David Gregory Belair ,  William T. Daly

IPC: C12N5/00

CPC classification number: A61L31/145 ,  A61K47/42 ,  A61L29/16 ,  A61L2300/80 ,  B01J19/0046 ,  B01J2219/00596 ,  C12N5/0606 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2537/10 ,  G01N21/6452 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2610/00

Abstract: Hydrogel Compositions and methods of using hydrogel compositions are disclosed. Advantageously, the hydrogel compositions offer the ability to rapidly screen substrate components for influencing cell attachment, spreading, proliferation, migration, and differentiation. In particularly suitable embodiments, the hydrogel compositions of the present disclosure may be used to promote tubulogenesis of endothelial cells.

Abstract translation: 公开了水凝胶组合物和使用水凝胶组合物的方法。 有利地，水凝胶组合物提供快速筛选底物组分以影响细胞附着，扩散，增殖，迁移和分化的能力。 在特别合适的实施方案中，本公开的水凝胶组合物可用于促进内皮细胞的细胞生成。

公开(公告)号：US20190316094A1

公开(公告)日：2019-10-17

申请号：US16385543

申请日：2019-04-16

Applicant: Wisconsin Alumni Research Foundation

Inventor： William T. Daly ,  William L. Murphy ,  Cheryl M. Soref ,  Elizabeth E. Torr ,  Elizabeth A. Aisenbrey ,  Hunter J. Johnson

IPC: C12N5/071 ,  C12N5/00 ,  G01N33/50

Abstract: The present invention provides methods of differentiating pericytes from pluripotent stem cells comprising culturing steps in chemically defined culture medium. A population of exogenously derived pericytes from PSCs are also contemplated. Further uses of the exogenously cultured pericytes for an in vitro disease model or in vitro angiogenesis assay are contemplated, including an in vitro 3D model of vasculature.