公开(公告)号：US10724002B2

公开(公告)日：2020-07-28

申请号：US15946101

申请日：2018-04-05

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC: C12N5/078

Abstract: Methods and compositions of erythroid cells that produce adult β-hemoglobin, generated by culturing CD31+, CD31+/CD34+ or CD34+ cells from embryonic stem cells under serum-free culture conditions.

公开(公告)号：US08785189B2

公开(公告)日：2014-07-22

申请号：US13859228

申请日：2013-04-09

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC: C12N5/071 ,  C12N5/00 ,  C12N5/02 ,  C12N5/0784

CPC classification number: C12N5/0639 ,  C12N5/064 ,  C12N2506/02

Abstract: This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.

Abstract translation: 本发明涉及来自人胚胎干（ES）细胞的树突状细胞的培养。 首先通过与基质细胞共培养将人类ES细胞培养成造血细胞。 然后将分化成造血谱系的细胞与GM-CSF一起培养以产生骨髓前体细胞的培养物。 用细胞因子GM-CSF和IL-4培养骨髓前体细胞会导致产生功能性树突状细胞。 树突细胞具有独特的表型，如细胞表面标志物的组合所示。

公开(公告)号：US20180230430A1

公开(公告)日：2018-08-16

申请号：US15946101

申请日：2018-04-05

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC: C12N5/078

CPC classification number: C12N5/0641 ,  C12N2500/25 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/23 ,  C12N2501/39 ,  C12N2502/1394 ,  C12N2506/02

Abstract: Methods and compositions of erythroid cells that produce adult β-hemoglobin, generated by culturing CD31+, CD31+/CD34+ or CD34+ cells from embryonic stem cells under serum-free culture conditions.

公开(公告)号：US20130230917A1

公开(公告)日：2013-09-05

申请号：US13859228

申请日：2013-04-09

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC: C12N5/0784

CPC classification number: C12N5/0639 ,  C12N5/064 ,  C12N2506/02

Abstract: This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.

Abstract translation: 本发明涉及来自人胚胎干（ES）细胞的树突状细胞的培养。 首先通过与基质细胞共培养将人类ES细胞培养成造血细胞。 然后将分化成造血谱系的细胞与GM-CSF一起培养以产生骨髓前体细胞的培养物。 用细胞因子GM-CSF和IL-4培养骨髓前体细胞会导致产生功能性树突状细胞。 树突细胞具有独特的表型，如细胞表面标志物的组合所示。