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    METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS
    11.
    发明申请
    METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS 有权

    公开(公告)号：US20230058778A1

    公开(公告)日：2023-02-23

    申请号：US17820202

    申请日：2022-08-16

    申请人： Life Technologies Corporation

    发明人： Jonathan ROTHBERG ,  Wolfgang HINZ ,  Kim JOHNSON ,  James BUSTILLO

    IPC分类号： G01N27/414 ,  C12Q1/6874 ,  H01L29/78 ,  H01L27/088 ,  C12Q1/6818 ,  G01N33/543

    摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

    CHEMICALLY-SENSITIVE SENSOR ARRAY CALIBRATION CIRCUITRY
    12.
    发明申请
    CHEMICALLY-SENSITIVE SENSOR ARRAY CALIBRATION CIRCUITRY 有权

    公开(公告)号：US20210109060A1

    公开(公告)日：2021-04-15

    申请号：US17067538

    申请日：2020-10-09

    申请人： Life Technologies Corporation

    发明人： Jonathan M. ROTHBERG ,  Wolfgang HINZ ,  Kim L. JOHNSON ,  James BUSTILLO

    IPC分类号： G01N27/414 ,  C12Q1/6874 ,  H01L29/78 ,  H01L27/088 ,  C12Q1/6818 ,  G01N33/543

    摘要： Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

    NUCLEIC ACID AMPLIFICATION
    16.
    发明申请
    NUCLEIC ACID AMPLIFICATION 审中-公开

    公开(公告)号：US20160272954A1

    公开(公告)日：2016-09-22

    申请号：US15091717

    申请日：2016-04-06

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Bin LI ,  Kai Qin LAO ,  Wolfgang HINZ

    IPC分类号： C12N9/12 ,  C12Q1/68

    CPC分类号： C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Y207/07007 ,  C12Q2531/119 ,  C12Q2565/537

    摘要： In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    SEQUENCING METHODS, COMPOSITIONS AND SYSTEMS
    17.
    发明申请
    SEQUENCING METHODS, COMPOSITIONS AND SYSTEMS 审中-公开
    序列方法，组成和系统

    公开(公告)号：US20160097091A1

    公开(公告)日：2016-04-07

    申请号：US14856220

    申请日：2015-09-16

    申请人： LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GmbH

    发明人： Wolfgang HINZ ,  Peter VANDER HORN ,  Earl HUBBELL ,  Christian WOEHLER

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  C12Q2535/113 ,  C12Q2565/519

    摘要： In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.

    摘要翻译： 在一些实施方案中，本公开一般涉及用于进行核苷酸掺入的方法以及组合物，系统，试剂盒和装置，其包括：（a）提供包含一个或多个含有聚合酶和核酸模板的反应位点的表面，所述反应位点 已经或被杂交到可延伸的一端; （b）通过使一个或多个所述反应位点与包含一种或多种类型的终止子核苷酸的第一溶液接触来进行第一核苷酸流; （c）使用所述聚合酶在至少一个所述反应位点内的所述核酸模板的可延伸末端掺入至少一种类型的终止子核苷酸; 和（d）使用附着或可操作地连接至少一个反应位点的传感器检测表示核苷酸掺入的非光学信号。

    SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING
    19.
    发明申请
    SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING 审中-公开
    SCAFFEDED核酸聚合物颗粒及其制备和使用方法

    公开(公告)号：US20140128269A1

    公开(公告)日：2014-05-08

    申请号：US14044712

    申请日：2013-10-02

    申请人： LIFE TECHNOLOGIES CORPORATION

    发明人： Wolfgang HINZ ,  John LEAMON ,  David LIGHT ,  Jonathan M. ROTHBERG

    IPC分类号： C12Q1/68

    CPC分类号： B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/1093 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

    摘要： The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

    摘要翻译： 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。