公开(公告)号：US20180119217A1

公开(公告)日：2018-05-03

申请号：US15561445

申请日：2016-03-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GMBH

Inventor： Wolfgang HINZ ,  Steven MENCHEN ,  Ronald GRAHAM ,  Peter VANDER HORN ,  Earl HUBBELL ,  Christian WOEHLER ,  Roman ROZHKOV ,  Barnett ROSENBLUM

IPC: C12Q1/6874

CPC classification number: C12Q1/6874 ,  C12Q2565/607

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.

公开(公告)号：US20160097091A1

公开(公告)日：2016-04-07

申请号：US14856220

申请日：2015-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES GmbH

Inventor： Wolfgang HINZ ,  Peter VANDER HORN ,  Earl HUBBELL ,  Christian WOEHLER

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2535/113 ,  C12Q2565/519

Abstract: In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.

Abstract translation: 在一些实施方案中，本公开一般涉及用于进行核苷酸掺入的方法以及组合物，系统，试剂盒和装置，其包括：（a）提供包含一个或多个含有聚合酶和核酸模板的反应位点的表面，所述反应位点 已经或被杂交到可延伸的一端; （b）通过使一个或多个所述反应位点与包含一种或多种类型的终止子核苷酸的第一溶液接触来进行第一核苷酸流; （c）使用所述聚合酶在至少一个所述反应位点内的所述核酸模板的可延伸末端掺入至少一种类型的终止子核苷酸; 和（d）使用附着或可操作地连接至少一个反应位点的传感器检测表示核苷酸掺入的非光学信号。

公开(公告)号：US20210143201A1

公开(公告)日：2021-05-13

申请号：US16624767

申请日：2018-06-18

Applicant: LIFE TECHNOLOGIES GmbH

Inventor： Christian WOEHLER

IPC: H01L27/146 ,  G02B6/10 ,  G02B5/20

Abstract: An image sensor for recording incident radiation may include a first layer for filtering the incident radiation by attenuating incident radiation with a frequency below a cutoff frequency and a second light-sensitive layer for absorbing radiation passing through the first layer. The first layer may precede the second light-sensitive layer in a direction of propagation of the incident radiation and the first layer includes at least one aperture passing through the first layer to the second light-sensitive layer for propagating radiation therethrough. The cross sectional size of the at least one aperture may be configured to provide a cutoff frequency so that incident radiation with a frequency below the cutoff frequency is attenuated inside the at least one aperture and incident radiation with a frequency above the cutoff frequency propagates through the at least one aperture.