公开(公告)号：US20200263972A1

公开(公告)日：2020-08-20

申请号：US16061499

申请日：2016-12-07

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Hidenao YAMADA

IPC: G01B9/02 ,  G01N21/45 ,  G02B21/14 ,  G02B21/18

Abstract: An interference observation apparatus includes a light source, a splitting beam splitter, a combining beam splitter, a mirror, a beam splitter, a mirror, a piezo element, a stage, a photodetector, an image acquisition unit, and a current control unit. An interference optical system from the splitting beam splitter to the combining beam splitter forms a Mach-Zehnder interferometer. The current control unit controls a wavelength of laser light output from the light source to adjust a phase difference between two split light components at the combining by the combining beam splitter.

公开(公告)号：US20160313182A1

公开(公告)日：2016-10-27

申请号：US15131390

申请日：2016-04-18

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Yoichi KAWADA ,  Yoshiyuki SHIMIZU ,  Toyohiko YAMAUCHI

IPC: G01J3/02 ,  G01J3/42

CPC classification number: G01J3/0229 ,  G01J3/42 ,  G01J2003/1213 ,  G01N2021/3595

Abstract: A spectroscopic measurement apparatus includes a light source, a diffraction grating being a spectroscopic unit, a spatial filter unit, a detection unit, and an analysis unit. The diffraction grating spatially disperses light from the light source, and outputs the light to different optical paths according to a wavelength. The spatial filter unit inputs the light from the diffraction grating to different positions according to the wavelength, applies loss depending on the wavelength to the light, and outputs the light. The detection unit detects the intensity of the light from the spatial filter unit. The analysis unit obtains the intensities of light in an absorption band and light in a non-absorption band of a component in a measurement sample on an optical path between the light source and the detection unit based on the detection result, and evaluates the component in the measurement sample.

Abstract translation: 光谱测量装置包括光源，衍射光栅是分光单元，空间滤光单元，检测单元和分析单元。 衍射光栅在空间上分散来自光源的光，并根据波长将光输出到不同的光路。 空间滤波器单元根据波长将来自衍射光栅的光输入到不同的位置，根据对光的波长施加损耗，并输出光。 检测单元检测来自空间滤波器单元的光的强度。 分析单元基于检测结果，在光源和检测单元之间的光路上获得吸收带中的光的强度和测量样本中的成分的非吸收带中的光，并且评估其中的分量 测量样品。

公开(公告)号：US20250003949A1

公开(公告)日：2025-01-02

申请号：US18739556

申请日：2024-06-11

Applicant: Hamamatsu Photonics K.K.

Inventor： Tokio TAKAGI ,  Toyohiko YAMAUCHI ,  Tatsuo DOUGAKIUCHI ,  Yoichi KAWADA ,  Yoshiyuki SHIMIZU ,  Gen TAKEBE

IPC: G01N33/483 ,  G01N1/28 ,  G01N1/30 ,  G01N21/64

Abstract: A cell sample measurement apparatus includes a sample stage for placing an object cell sample containing a plurality of cells cultured on a culture dish, an evaluation light irradiation unit for irradiating N irradiation regions set by dividing a culture region in the object cell sample with evaluation light having a wavelength in a mid-infrared region with irradiation amounts different from each other, a dead cell number measurement unit for measuring a dead cell number generated according to the irradiation amount of the evaluation light in each of the N irradiation regions, and a culture state analysis unit for obtaining, for the object cell sample, an object correlation between the irradiation amount of the evaluation light and the dead cell number for the N irradiation regions serving as an index of evaluation of a culture state of the object cell sample.

公开(公告)号：US20200318942A1

公开(公告)日：2020-10-08

申请号：US16954223

申请日：2018-10-11

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Hidenao YAMADA ,  Kentaro GOTO

IPC: G01B9/02 ,  G01N21/01 ,  G01N21/45

Abstract: Provided is an observation target cover for interference observation using first light and second light having a coherent length longer than that of the first light so as to acquire an interference light image of an observation target by the first light while adjusting an optical path length difference based on an interference result of the second light, the observation target cover including: a transmission reflection portion that transmits the first light and reflects the second light; and a support portion for supporting the transmission reflection portion so that a placement surface on which the observation target is placed and the transmission reflection portion face each other with a predetermined gap therebetween.

公开(公告)号：US20180236257A1

公开(公告)日：2018-08-23

申请号：US15896267

申请日：2018-02-14

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Gen TAKEBE ,  Yoshiyuki SHIMIZU ,  Toyohiko YAMAUCHI ,  Tatsuo DOUGAKIUCHI

IPC: A61N5/06 ,  C12M1/42 ,  C12N13/00

CPC classification number: A61N5/062 ,  A61N5/0622 ,  A61N2005/0659 ,  C12M35/02 ,  C12N13/00 ,  C12N2529/10

Abstract: A cell stimulation method includes continuously emitting mid-infrared light to a living cell and thus changing an ion concentration of the cell or changing ion concentrations of the cell and other cells disposed around the cell.

公开(公告)号：US20180017371A1

公开(公告)日：2018-01-18

申请号：US15546168

申请日：2015-12-14

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Hidenao YAMADA

IPC: G01B9/02 ,  G01N21/45

CPC classification number: G01B9/0203 ,  G01B9/02015 ,  G01B9/02042 ,  G01B9/02049 ,  G01N21/45 ,  G01N33/4833 ,  G02B21/00 ,  G02B21/06 ,  G02B21/361

Abstract: An interference observation apparatus includes a light source which outputs incoherent light, a beam splitter, a sample holding table, an objective lens, a reference mirror, a lens, an aberration correction plate, a piezo element, a tube lens, a beam splitter, an imaging unit, a photodetector, an image acquisition unit, and a control unit. The control unit obtains an interference intensity of combined light on the basis of a detection signal output from the photodetector, and adjusts an interference optical system to increase the interference intensity.

公开(公告)号：US20170212033A1

公开(公告)日：2017-07-27

申请号：US15329456

申请日：2015-07-23

Applicant: NATIONAL UNIVERSITY CORPORATION HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE ,  HAMAMATSU PHOTONICS K.K.

Inventor： Yusuke OZAKI ,  Hidenao IWAI ,  Hiroyuki KONNO ,  Hirotoshi KIKUCHI ,  Toyohiko YAMAUCHI

IPC: G01N15/14 ,  G01B11/06

CPC classification number: G01N15/1429 ,  G01B11/06 ,  G01N15/0227 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1463 ,  G01N15/1475 ,  G01N2015/008 ,  G01N2015/025 ,  G01N2015/1018 ,  G01N2015/1493 ,  G06K9/00147

Abstract: The identification apparatus includes a quantitative phase image acquisition unit, a feature quantity extraction unit, a learning unit, a storage unit, and an identification unit. The feature quantity extraction unit extracts a feature quantity of a quantitative phase image of a cell. The learning unit performs machine learning for a quantitative phase image of a known cell of which a type is known based on the feature quantity extracted by the extraction unit. The storage unit stores a result of the machine learning by the learning unit. The identification unit determines, based on the feature quantity extracted by the extraction unit for the quantitative phase image of an unknown cell of which a type is unknown, the type of the unknown cell using the learning result stored by the storage unit.

公开(公告)号：US20160334384A1

公开(公告)日：2016-11-17

申请号：US15218369

申请日：2016-07-25

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Toyohiko YAMAUCHI ,  Norikazu SUGIYAMA ,  Tadashi FUKAMI ,  Hidenao IWAI

IPC: G01N33/483 ,  G06T7/00 ,  C12N5/074

CPC classification number: G01N33/4833 ,  C12N5/0696 ,  C12Q1/04 ,  G01N15/1456 ,  G01N2015/1006 ,  G01N2015/1497 ,  G06T7/0012 ,  G06T7/50 ,  G06T2207/10056 ,  G06T2207/30004

Abstract: The present invention provides a method for determining differentiation level of pluripotent stem cell, comprising a step of determining a flatness of cultured pluripotent stem cell, wherein the flatness is an indication.

Abstract translation: 本发明提供了一种确定多能干细胞分化水平的方法，包括确定培养的多能干细胞的平坦度的步骤，其中平坦度是指示。

公开(公告)号：US20150369790A1

公开(公告)日：2015-12-24

申请号：US14766238

申请日：2014-02-05

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Hidenao IWAI ,  Takuji KATAOKA ,  Satoshi YAMAMOTO ,  Norikazu SUGIYAMA ,  Toyohiko YAMAUCHI ,  Kentaro GOTO

IPC: G01N33/483 ,  G01N21/47

CPC classification number: G01N33/4833 ,  G01B11/00 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4707 ,  G01N2021/479

Abstract: An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kn of a speckle image In at each time tn, determines a maximum value Kmax among the speckle contrast values K1 to KN, and normalizes the speckle contrast value Kn at each time tn by the maximum value Kmax to obtain a normalized speckle contrast value Kn′. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value Kn′ at each time tn.

Abstract translation: 聚合单元评估装置包括激光光源，散斑图像获取单元，SC计算单元，评估单元和存储单元。 斑点图像获取单元通过从激光光源输出的激光照射聚集单元，在聚集单元中产生的前向散射光获取二维散斑图像。 SC计算单元计算每次tn时的散斑图像In的散斑对比度值Kn，确定散斑对比度值K 1〜K N的最大值Kmax，并将每个时间tn处的散斑对比度值Kn标准化最大值Kmax 以获得归一化散斑对比度值Kn'。 评估单元基于每个时刻tn处的归一化散斑对比度值Kn'评估聚集单元的运动。