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公开(公告)号：US20210102235A1
公开(公告)日：2021-04-08
申请号：US16499957
申请日：2018-03-13
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Kentaro GOTO , Toyohiko YAMAUCHI , Hidenao YAMADA
Abstract: An evaluation method is a method for evaluating a cell mass containing a plurality of aggregated cells, and includes an imaging step of capturing an image of light obtained from the cell mass by irradiating the cell mass with light, an analysis step of setting a reference point for the cell mass included in image data obtained by the imaging step, setting sampling circles centered on the reference point, and determining a parameter for a state of the cell mass based on image data included in regions on the sampling circles, and an evaluation step of evaluating the cell mass based on the parameter.
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公开(公告)号：US20170356735A1
公开(公告)日：2017-12-14
申请号：US15545356
申请日：2015-12-14
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Toyohiko YAMAUCHI , Hidenao YAMADA
IPC: G01B9/02 , G01N21/45 , G02B21/06 , G01N33/483 , G02B21/36
CPC classification number: G01B9/0203 , G01B9/02015 , G01B9/02042 , G01B9/02049 , G01N21/45 , G01N33/4833 , G02B21/00 , G02B21/06 , G02B21/361
Abstract: An interference observation apparatus includes a light source, a splitting beam splitter, a combining beam splitter, a beam splitter, a mirror, a beam splitter, a mirror, a piezo element, a stage, an imaging unit, an image acquisition unit, and a control unit. An interference optical system from the splitting beam splitter to the combining beam splitter forms a Mach-Zehnder interferometer. The mirror freely moves in a direction perpendicular to a reflecting surface of the mirror. The total number of times of respective reflections of first split light and second split light in the interference optical system is an even number.
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3.发明申请CELL ASSESSMENT METHOD, CELL ASSESSMENT DEVICE, AND CELL ASSESSMENT PROGRAM 审中-公开细胞评估方法，细胞评估装置和细胞评估方案公开(公告)号：US20160349170A1
公开(公告)日：2016-12-01
申请号：US15037142
申请日：2014-10-17
Applicant: KYOTO UNIVERSITY , HAMAMATSU PHOTONICS K.K.
Inventor： Norio NAKATSUJI , Norikazu SUGIYAMA , Yoshinori MIZUGUCHI , Tadashi FUKAMI , Hidenao YAMADA , Toyohiko YAMAUCHI , Yumi KAKUNO
CPC classification number: G01N15/1434 , C12M41/36 , G01N15/1475 , G01N33/5073 , G01N2015/1006 , G01N2015/144 , G01N2015/1447 , G06K9/00134 , G06K9/0014 , G06K9/00147
Abstract: The present invention relates to a cell assessment method characterized in including an acquisition step of acquiring an optical path length image of a small cell clump, an extraction step of extracting a cell nucleus region within the acquired optical path length image, a comparison step of comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment step of assessing whether or not a cell is a stem cell based on the comparison results.
Abstract translation: 本发明涉及一种细胞评估方法，其特征在于包括获取小细胞团的光程长度图像的获取步骤，在获取的光程长图像内提取细胞核区域的提取步骤，比较步骤 提取的细胞核区域的内侧的光路长度和光路长度，以及基于比较结果来评价细胞是否为干细胞的评价步骤。
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公开(公告)号：US20200080833A1
公开(公告)日：2020-03-12
申请号：US16561059
申请日：2019-09-05
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Toyohiko YAMAUCHI , Osamu YASUHIKO , Hidenao YAMADA , Hisayuki MATSUI
Abstract: A measurement apparatus includes an interference image acquisition unit, a fluorescence image acquisition unit, an operation unit, and a timing control circuit. The operation unit generates an optical thickness image based on an interference image acquired by the interference image acquisition unit, generates a mask image showing a region in which pixel values in a fluorescence image acquired by the fluorescence image acquisition unit are larger than a threshold value, and determines an integrated value of an optical thickness in the region shown by the mask image in the optical thickness image.
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5.发明申请公开(公告)号：US20180017370A1
公开(公告)日：2018-01-18
申请号：US15546126
申请日：2015-12-14
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Toyohiko YAMAUCHI , Hidenao YAMADA
Abstract: An interference observation apparatus includes an interference optical apparatus , a microscope housing, an imaging unit, and an objective lens. The interference optical apparatus includes a housing, a light source, a photodetector, beam splitters, a reference mirror, and a control unit. The interference observation apparatus is configured such that the housing of the interference optical apparatus is disposed between the objective lens attachment portion and the objective lens in a microscope apparatus including the imaging unit for capturing an image of the light passing through the objective lens attached to the objective lens attachment portion having an opening.
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Patent Agency Ranking
公开(公告)号：US20150219543A1
公开(公告)日：2015-08-06
申请号：US14613672
申请日：2015-02-04
Applicant: HAMAMATSU PHOTONICS K.K. , Kyoto University
Inventor： Toyohiko YAMAUCHI , Tadashi FUKAMI , Norikazu SUGIYAMA , Hidenao IWAI , Yumi KAKUNO , Kentaro GOTO , Norio NAKATSUJI , Kazuhiro AIBA
CPC classification number: G01N15/10 , G01N15/00 , G01N15/1475 , G01N2015/0065 , G01N2015/1087 , G06K9/00127 , G06T7/0012 , G06T2207/10056 , G06T2207/30024
Abstract: The present invention relates to a cell evaluation method comprising evaluating a cell differentiation degree based on a cell thickness.
Abstract translation: 本发明涉及一种细胞评估方法，其包括基于细胞厚度评估细胞分化程度。
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公开(公告)号：US20190072477A1
公开(公告)日：2019-03-07
申请号：US16084307
申请日：2017-03-02
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Hidenao YAMADA , Toyohiko YAMAUCHI
IPC: G01N15/14
Abstract: An observation apparatus observes an observation object moving in a flow path with a fluid, and includes a light source, a splitting unit, a combining unit, a collimator, a cylindrical lens, an objective lens, a collimator, a cylindrical lens, an objective lens, a modulation unit, an imaging unit, an analysis unit, and the like. The imaging unit includes a plurality of pixels arranged in a direction intersecting with a moving direction of an image of the observation object on a light receiving plane on which the image of the observation object moving in the flow path is formed, and receives combined light output from the combining unit to repeatedly output a detection signal indicating a one-dimensional interference image. The analysis unit generates a two-dimensional image of the observation object on the basis of the detection signal.
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公开(公告)号：US20140073002A1
公开(公告)日：2014-03-13
申请号：US14023735
申请日：2013-09-11
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Toyohiko YAMAUCHI , Norikazu SUGIYAMA , Tadashi FUKAMI , Hidenao IWAI
IPC: G01N33/483
CPC classification number: G01N33/4833 , C12N5/0696 , C12Q1/04 , G01N15/1456 , G01N2015/1006 , G01N2015/1497 , G06T7/0012 , G06T7/50 , G06T2207/10056 , G06T2207/30004
Abstract: The present invention provides a method for determining differentiation level of pluripotent stem cell, comprising a step of determining a flatness of cultured pluripotent stem cell, wherein the flatness is an indication.
Abstract translation: 本发明提供了一种确定多能干细胞分化水平的方法，包括确定培养的多能干细胞的平坦度的步骤，其中平坦度是指示。
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公开(公告)号：US20250002836A1
公开(公告)日：2025-01-02
申请号：US18739573
申请日：2024-06-11
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Tokio TAKAGI , Toyohiko YAMAUCHI , Tatsuo DOUGAKIUCHI , Yoichi KAWADA , Yoshiyuki SHIMIZU , Gen TAKEBE
Abstract: A cell sample evaluation apparatus includes an evaluation light irradiation unit for irradiating an irradiation region set in a culture region of a plurality of cells in an object cell sample with evaluation light having a wavelength in a mid-infrared region under a predetermined irradiation condition, a dead cell number measurement unit for measuring a dead cell number generated according to an irradiation amount of the evaluation light in the irradiation region of the object cell sample, and a culture state analysis unit for evaluating a culture state of the object cell sample by obtaining an object correlation between the irradiation amount of the evaluation light and the dead cell number for the object cell sample and comparing a reference correlation between the irradiation amount of the evaluation light and the dead cell number for a reference cell sample and the object correlation.
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公开(公告)号：US20200333250A1
公开(公告)日：2020-10-22
申请号：US16959241
申请日：2018-10-30
Applicant: HAMAMATSU PHOTONICS K.K.
Inventor： Toyohiko YAMAUCHI , Hidenao YAMADA
Abstract: A fluorescence measurement apparatus includes a fluorescence image acquisition unit that acquires a fluorescence image containing an object, an interference image acquisition unit that acquires an interference image containing the object, and an operation unit. The operation unit determines an optical thickness image based on the interference image acquired by the interference image acquisition unit, and determines, in a region of interest set in common in both of the fluorescence image acquired by the fluorescence image acquisition unit and the optical thickness image, a fluorescence expression rate of the object based on an integrated value of a fluorescence intensity in the fluorescence image and an integrated value of an optical thickness in the optical thickness image.
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