公开(公告)号：US20250002836A1

公开(公告)日：2025-01-02

申请号：US18739573

申请日：2024-06-11

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Tokio TAKAGI ,  Toyohiko YAMAUCHI ,  Tatsuo DOUGAKIUCHI ,  Yoichi KAWADA ,  Yoshiyuki SHIMIZU ,  Gen TAKEBE

IPC: C12M1/34 ,  C12M1/00 ,  G01N1/30 ,  G01N21/25

Abstract: A cell sample evaluation apparatus includes an evaluation light irradiation unit for irradiating an irradiation region set in a culture region of a plurality of cells in an object cell sample with evaluation light having a wavelength in a mid-infrared region under a predetermined irradiation condition, a dead cell number measurement unit for measuring a dead cell number generated according to an irradiation amount of the evaluation light in the irradiation region of the object cell sample, and a culture state analysis unit for evaluating a culture state of the object cell sample by obtaining an object correlation between the irradiation amount of the evaluation light and the dead cell number for the object cell sample and comparing a reference correlation between the irradiation amount of the evaluation light and the dead cell number for a reference cell sample and the object correlation.

公开(公告)号：US20240319202A1

公开(公告)日：2024-09-26

申请号：US18591189

申请日：2024-02-29

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Gen TAKEBE ,  Shigetoshi OKAZAKI

IPC: G01N33/68 ,  C09B11/06 ,  C09K11/06 ,  G01N21/31 ,  G01N21/64

CPC classification number: G01N33/68 ,  C09B11/06 ,  C09K11/06 ,  G01N21/3103 ,  G01N21/6428 ,  C09K2211/1007 ,  C09K2211/1018 ,  G01N2021/6439

Abstract: A method for evaluating an aggregation state of aggregative proteins in a solution is disclosed, the method comprising evaluating an aggregation state of aggregative proteins using, as an index, light-absorption/fluorescence characteristic(s) of a certain xanthene dye in a solution comprising the aggregative proteins and the xanthene dye or a salt thereof.

公开(公告)号：US20250003949A1

公开(公告)日：2025-01-02

申请号：US18739556

申请日：2024-06-11

Applicant: Hamamatsu Photonics K.K.

Inventor： Tokio TAKAGI ,  Toyohiko YAMAUCHI ,  Tatsuo DOUGAKIUCHI ,  Yoichi KAWADA ,  Yoshiyuki SHIMIZU ,  Gen TAKEBE

IPC: G01N33/483 ,  G01N1/28 ,  G01N1/30 ,  G01N21/64

Abstract: A cell sample measurement apparatus includes a sample stage for placing an object cell sample containing a plurality of cells cultured on a culture dish, an evaluation light irradiation unit for irradiating N irradiation regions set by dividing a culture region in the object cell sample with evaluation light having a wavelength in a mid-infrared region with irradiation amounts different from each other, a dead cell number measurement unit for measuring a dead cell number generated according to the irradiation amount of the evaluation light in each of the N irradiation regions, and a culture state analysis unit for obtaining, for the object cell sample, an object correlation between the irradiation amount of the evaluation light and the dead cell number for the N irradiation regions serving as an index of evaluation of a culture state of the object cell sample.

公开(公告)号：US20180236257A1

公开(公告)日：2018-08-23

申请号：US15896267

申请日：2018-02-14

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Gen TAKEBE ,  Yoshiyuki SHIMIZU ,  Toyohiko YAMAUCHI ,  Tatsuo DOUGAKIUCHI

IPC: A61N5/06 ,  C12M1/42 ,  C12N13/00

CPC classification number: A61N5/062 ,  A61N5/0622 ,  A61N2005/0659 ,  C12M35/02 ,  C12N13/00 ,  C12N2529/10

Abstract: A cell stimulation method includes continuously emitting mid-infrared light to a living cell and thus changing an ion concentration of the cell or changing ion concentrations of the cell and other cells disposed around the cell.

公开(公告)号：US20130187050A1

公开(公告)日：2013-07-25

申请号：US13742616

申请日：2013-01-16

Applicant: Hamamatsu Photonics K.K.

Inventor： Gen TAKEBE ,  Yoichi KAWADA ,  Kouichiro AKIYAMA

IPC: G01N21/35

CPC classification number: G01N21/3577 ,  G01N21/3563 ,  G01N21/3586 ,  G01N21/552

Abstract: The drug evaluation device obtains, by an attenuated reflection method using a terahertz wave, an evaluation absorption spectrum for a frequency with respect to a liquid to be evaluated. When crystalline particles are suspended in a liquid, an absorption peak having a peak area corresponding to the amount of suspension appears in its absorption spectrum. Therefore, whether or not and by what ratio crystalline particles are suspended in the liquid can be determined according to whether or not the absorption peak exists and the peak area. When amorphous particles are suspended in the liquid, the baseline of its absorption spectrum lowers according to the ratio of amorphous particles suspended in the liquid. Therefore, whether or not and by what ratio amorphous particles are suspended in the liquid can be determined according to the lowering amount of the baseline.

Abstract translation: 药物评价装置通过使用太赫兹波的衰减反射法获得关于待评价液体的频率的评价吸收光谱。 当结晶颗粒悬浮在液体中时，具有对应于悬浮液量的峰面积的吸收峰出现在其吸收光谱中。 因此，可以根据是否存在吸收峰和峰面积来确定晶体颗粒悬浮在液体中的比例和比例。 当无定形颗粒悬浮在液体中时，其吸收光谱的基线根据悬浮在液体中的无定形颗粒的比例而降低。 因此，可以根据基线的降低量来确定无定形颗粒悬浮在液体中的比例和比例。