公开(公告)号：US20190352593A1

公开(公告)日：2019-11-21

申请号：US16527423

申请日：2019-07-31

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Natsumi SAITO ,  Taira ITO ,  Takuji KATAOKA

IPC: C12M1/42 ,  G01N33/487 ,  G01N33/483 ,  G01N21/64 ,  C12M1/34

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

公开(公告)号：US20160326480A1

公开(公告)日：2016-11-10

申请号：US15110678

申请日：2014-11-12

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Natsumi SAITO ,  Taira ITO ,  Takuji KATAOKA

IPC: C12M1/42 ,  G01N21/64 ,  G01N33/487

CPC classification number: C12M35/02 ,  C12M41/36 ,  G01N21/6452 ,  G01N33/4836 ,  G01N33/48707 ,  G01N33/48728

Abstract: A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.

Abstract translation: 细胞观察装置是细胞观察装置，用于观察由微孔板保持的细胞，所述微孔板具有保持包含细胞的样品的微孔板，并且包括用于在其上保持微孔板的微孔板保持器，电刺激单元包括电极对，其包括第一电极和 第二电极和位置控制器，用于当电极对设置在微孔板的孔中时，控制电刺激单元的位置处于第一电极设置成比阱的中心更靠近阱的中心的状态。 第一电极的尖端比第二电极的尖端延伸更多。

公开(公告)号：US20150369790A1

公开(公告)日：2015-12-24

申请号：US14766238

申请日：2014-02-05

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Hidenao IWAI ,  Takuji KATAOKA ,  Satoshi YAMAMOTO ,  Norikazu SUGIYAMA ,  Toyohiko YAMAUCHI ,  Kentaro GOTO

IPC: G01N33/483 ,  G01N21/47

CPC classification number: G01N33/4833 ,  G01B11/00 ,  G01N21/47 ,  G01N21/49 ,  G01N2021/4707 ,  G01N2021/479

Abstract: An aggregated cell evaluation apparatus includes a laser light source, a speckle image acquisition unit, an SC calculation unit, an evaluation unit, and a memory unit. The speckle image acquisition unit acquires a two-dimensional speckle image by forward scattered light generated in aggregated cells by irradiation of the aggregated cells with laser light output from the laser light source. The SC calculation unit calculates a speckle contrast value Kn of a speckle image In at each time tn, determines a maximum value Kmax among the speckle contrast values K1 to KN, and normalizes the speckle contrast value Kn at each time tn by the maximum value Kmax to obtain a normalized speckle contrast value Kn′. The evaluation unit evaluates motion of the aggregated cells, based on the normalized speckle contrast value Kn′ at each time tn.

Abstract translation: 聚合单元评估装置包括激光光源，散斑图像获取单元，SC计算单元，评估单元和存储单元。 斑点图像获取单元通过从激光光源输出的激光照射聚集单元，在聚集单元中产生的前向散射光获取二维散斑图像。 SC计算单元计算每次tn时的散斑图像In的散斑对比度值Kn，确定散斑对比度值K 1〜K N的最大值Kmax，并将每个时间tn处的散斑对比度值Kn标准化最大值Kmax 以获得归一化散斑对比度值Kn'。 评估单元基于每个时刻tn处的归一化散斑对比度值Kn'评估聚集单元的运动。

公开(公告)号：US20150323454A1

公开(公告)日：2015-11-12

申请号：US14806999

申请日：2015-07-23

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Norikazu SUGIYAMA ,  Takuji KATAOKA ,  Ikuo ARATA

IPC: G01N21/47 ,  G01N33/483

CPC classification number: G01N21/47 ,  G01N21/49 ,  G01N21/59 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2201/0446 ,  G01N2201/062 ,  G02B21/088 ,  G02B21/36

Abstract: A method for observing stem cells by an observation device 1 comprises, placing stem cells C in a petri dish 11, mounting the petri dish 11 on a waveguide 21 via water 13, emitting illumination light L1 into the waveguide 21 and emitting the illumination light L1 to the stem cells C in the petri dish 11 via the water 13, and detecting scattered light L2, the scattered light L2 being the illumination light L1 emitted to the stem cells C that is scattered by the stem cells C and has passed through the waveguide 21. Then, in the light image detected by means of the scattered light L2, a region that is markedly darker than other regions is identified as being in the state tending toward differentiation.

Abstract translation: 通过观察装置1观察干细胞的方法包括：将干细胞C放置在培养皿11中，通过水13将培养皿11安装在波导21上，将照明光L1发射到波导21中并发射照明光L1 通过水13向陪替氏培养皿11中的干细胞C，并且检测散射光L2，散射光L2是发射到干细胞C的干细胞C的照射光L1，其被干细胞C散射并已经通过波导 然后，在通过散射光L2检测到的光图像中，比其他区域明显更暗的区域被识别为处于朝向微分的状态。