公开(公告)号：US20240052300A1

公开(公告)日：2024-02-15

申请号：US18266689

申请日：2021-12-21

申请人： AMGEN RESEARCH (MUNCH) GMBH ,  AMGEN INC.

发明人： Glenn Christopher Tan ,  Ewelina Zasadzinska ,  Patrick Hoffmann ,  Bin Wu

IPC分类号： C12N5/00 ,  C12M3/06

CPC分类号： C12N5/0075 ,  C12M23/16

摘要： The present disclosure provides materials and methods identifying, selecting, and characterizing cells that express and secrete non-Fc containing biomolecules.

公开(公告)号：US11898164B2

公开(公告)日：2024-02-13

申请号：US16474662

申请日：2017-12-20

申请人： JEIL PHARMACEUTICAL CO., LTD.

发明人： Myung Soo Cho ,  Mi Sun Lim ,  So Yeon Ji

IPC分类号： C12N5/00 ,  C12N5/0793 ,  C12N5/0797

CPC分类号： C12N5/0619 ,  C12N5/0623 ,  C12N2501/115 ,  C12N2506/08 ,  C12N2506/45 ,  C12N2533/20 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

摘要： Disclosed is a method for producing a spherical neural mass having suppressed teratoma formation. When using the spherical neural mass produced according to the method of the present disclosure, the purity of the neuronal progenitor cells may be improved, the teratoma formation may be suppressed, and the viability and recovery percentage of the cell may be increased.

公开(公告)号：US11896662B2

公开(公告)日：2024-02-13

申请号：US17534659

申请日：2021-11-24

申请人： Novavax, Inc.

发明人： Sarathi Boddapati ,  Anushree Herwadkar ,  Jason Wong ,  Yen-Huei Lin ,  Gale Smith ,  Jing-Hui Tian

IPC分类号： A61K39/12 ,  C12N5/00 ,  A61K39/145 ,  A61P31/16 ,  A61K9/00 ,  A61K9/51 ,  A61K39/00

CPC分类号： A61K39/145 ,  A61K9/0051 ,  A61K9/51 ,  A61P31/16 ,  A61K2039/70

摘要： Disclosed herein are multivalent nanoparticle vaccine compositions suitable for use in influenza vaccines. The nanoparticles include effective amounts of influenza glycoproteins that provide increased immune responses compared to a commercially available influenza vaccine composition. The present disclosure also provides vaccine formulation strategies that are cost effective and are convenient for clinical use. Methods of administering the nanoparticle vaccine compositions to a subject are also disclosed.

公开(公告)号：US11891510B2

公开(公告)日：2024-02-06

申请号：US16645915

申请日：2018-12-06

申请人： Sigma-Aldrich Co. LLC

发明人： Scott Wilson ,  Kevin Kent ,  Chandana Sharma

IPC分类号： C08L71/02 ,  C08L71/00 ,  C12N5/00

CPC分类号： C08L71/02 ,  C08L71/00 ,  C12N5/0043

摘要： Described herein are poloxamer compositions for use as a shear protectant in cell culture and methods for preparing and using such compositions.

公开(公告)号：US11884934B2

公开(公告)日：2024-01-30

申请号：US16632717

申请日：2018-07-20

申请人： Washington University

发明人： Eynav Klechevsky ,  Amit Pathak ,  Bapi Sarker

IPC分类号： C12N5/0784 ,  A61P35/00 ,  A61K9/50 ,  A61K35/17 ,  C12N5/00 ,  C12N5/0783 ,  A61K47/00 ,  A61K47/69 ,  C07K16/28 ,  C07K14/54 ,  C07K14/74 ,  C07K14/715

CPC分类号： C12N5/0639 ,  A61K9/5057 ,  A61K9/5068 ,  A61K9/5089 ,  A61K35/17 ,  A61K47/00 ,  A61K47/6903 ,  A61P35/00 ,  C07K14/5443 ,  C07K14/70539 ,  C07K14/7155 ,  C07K16/2818 ,  C12N5/0012 ,  C12N5/0638 ,  C12N2501/2315 ,  C12N2501/51 ,  C12N2501/998 ,  C12N2531/00 ,  C12N2533/54 ,  C12N2533/74 ,  C12N2537/10

摘要： Among the various aspects of the present disclosure is the provision of methods, synthetic DC, and compositions for T cell activation. The present disclosure provides for synthetic dendritic cells (DCs), methods of generating synthetic dendritic cells (DCs), methods of generating T cell-encapsulated gelatin microspheres and microcapsules, methods of activating T cells using synthetic DCs, methods for expanding T cells against individualized antigen-specific mutational antigens using synthetic DCs, and methods of treating a chronic disease (e.g., HIV, HPV) or cancer using the synthetic DCs.

公开(公告)号：US20240026270A1

公开(公告)日：2024-01-25

申请号：US18220535

申请日：2023-07-11

申请人： The University of North Carolina at Chapel Hill

发明人： Nancy Allbritton ,  Yuli Wang ,  Hennayaka Mudiyanselage Dulan Gunasekara ,  Christopher Sims

IPC分类号： C12M1/12 ,  C12M1/00 ,  C12M1/42 ,  C12N5/00 ,  C12N5/071 ,  G01N33/50

CPC分类号： C12M25/14 ,  C12M23/20 ,  C12M35/08 ,  C12N5/0062 ,  C12N5/0679 ,  G01N33/5014 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2537/10

摘要： The present invention is directed to a method of making a live cell construct or a support, comprising: (a) providing a non-cellular organic polymer support having a top surface, a bottom surface, and an intermediate portion there between, and (b) contacting a cross-linking agent to one surface of said support for a time sufficient to generate a gradient of cross-linking of said polymer in said intermediate portion. Also provided are live cell constructs, supports, and methods of use of the supports and live cell constructs.

公开(公告)号：US20240026265A1

公开(公告)日：2024-01-25

申请号：US18216230

申请日：2023-06-29

申请人： The University of North Carolina at Chapel Hill

发明人： Nancy L. Allbritton ,  Christopher E. Sims ,  Yuli Wang ,  Pavak Kirit Shah

IPC分类号： C12M1/32 ,  C12M1/00 ,  C12M1/12 ,  C12N1/02 ,  C12N5/00 ,  B01J19/00

CPC分类号： C12M23/12 ,  C12M47/02 ,  C12M47/04 ,  C12M25/04 ,  C12N1/02 ,  C12N5/0068 ,  C12M25/16 ,  B01J19/0046 ,  C12M23/20 ,  C12M23/22 ,  C12N2509/10 ,  B01J2219/00452 ,  B01J2219/00743

摘要： An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).

公开(公告)号：US20240018469A1

公开(公告)日：2024-01-18

申请号：US18374889

申请日：2023-09-29

申请人： UNIVERSITE DE BORDEAUX ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT D'OPTIQUE THEORIQUE ET APPLIQUEE

发明人： MAXIME FEYEUX ,  KEVIN ALESSANDRI ,  PIERRE NASSOY ,  LAURENT COGNET ,  GAELLE RECHER ,  ERWAN BEZARD

IPC分类号： C12N5/00 ,  C12M1/00 ,  C12M1/12 ,  C12N5/074

CPC分类号： C12N5/0075 ,  C12M23/20 ,  C12M25/01 ,  C12M25/16 ,  C12N5/0012 ,  C12N5/0696 ,  C12N2501/727 ,  C12N2513/00 ,  C12N2533/74 ,  C12N2533/90 ,  C12N2535/00

摘要： The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

公开(公告)号：US11865536B2

公开(公告)日：2024-01-09

申请号：US17730615

申请日：2022-04-27

申请人： President and Fellows of Harvard College

发明人： Girija Goyal ,  Donald E. Ingber

IPC分类号： B01L3/00 ,  C12N5/0781 ,  C12N5/0783 ,  A61K35/28 ,  A61P35/00 ,  A61K35/17 ,  C12M3/06 ,  A61P37/04 ,  C12M3/00 ,  A61P7/00 ,  C12N5/00 ,  C12N5/0786 ,  C12N5/078 ,  C12N5/071 ,  C12N5/09 ,  G01N33/50

CPC分类号： B01L3/5027 ,  A61K35/17 ,  A61K35/28 ,  A61P7/00 ,  A61P35/00 ,  A61P37/04 ,  C12M21/08 ,  C12M23/16 ,  C12N5/0018 ,  C12N5/069 ,  C12N5/0635 ,  C12N5/0636 ,  C12N5/0645 ,  C12N5/0651 ,  C12N5/0693 ,  G01N33/5011 ,  B01L2300/0861 ,  C12N2531/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

摘要： An organ-on-a-chip microfluidic device is disclosed that mimics a human lymph node and/or human lymphoid tissue. The device can include cells from human blood and lymphatic tissue, include an extracellular matrix for the development of immune system components, and provide for the perfusion of fluids and solids resembling blood and lymphatic fluid within micrometer sized channels.

公开(公告)号：US20240002778A1

公开(公告)日：2024-01-04

申请号：US18368281

申请日：2023-09-14

申请人： CORNING INCORPORATED

发明人： Gregory Roger Martin ,  Allison Jean Tanner

IPC分类号： C12M1/00 ,  C12M1/26 ,  C12N5/00

CPC分类号： C12M47/02 ,  C12M33/14 ,  C12N5/0075

摘要： A cell separation device configured for separating cells from microcarriers or spheroids in a liquid is provided. The cell separation device includes a vessel comprising a first port, a second port, and a cavity; and a porous mesh disposed within the cavity to divide the cavity into a first compartment and a second compartment, wherein the first port is in communication with the first compartment of the cavity, the first port located to a first side of the porous mesh, wherein the second port is in communication with the second compartment of the cavity, the second port located to a second side of the porous mesh, and wherein the porous mesh is positioned within the cavity to have a substantially vertical orientation or an inclined orientation with respect to a flow of liquid through the porous mesh.