公开(公告)号：US20240353325A1

公开(公告)日：2024-10-24

申请号：US18761357

申请日：2024-07-02

Applicant: Oxford University Innovation Limited

Inventor： Philipp KUKURA ,  Sanli FAEZ

IPC: G01N21/47 ,  G01N21/66 ,  G02B21/00 ,  G02B21/36

CPC classification number: G01N21/47 ,  G01N21/66 ,  G02B21/002 ,  G02B21/36 ,  G01N21/4795

Abstract: A scattering microscopy arrangement uses a microscope to image an object comprising a surface. A light source emits illuminating light and a light detector detects light elastically scattered from the object. An electric potential is applied to the surface that affects the electrochemical properties of the object while imaging. The electric potential provides a contrast mechanism that improves the imaging and allows for characterisation of the object and/or the surrounding environment.

公开(公告)号：US12061207B2

公开(公告)日：2024-08-13

申请号：US18063500

申请日：2022-12-08

Applicant: SCOPIO LABS LTD.

Inventor： Itai Hayut ,  Erez Na'aman ,  Eran Small

IPC: G02B21/26 ,  G01N35/00 ,  G02B21/34 ,  G02B21/00

CPC classification number: G01N35/00029 ,  G02B21/34 ,  G01N2035/00039 ,  G01N2035/00089 ,  G02B21/002

Abstract: A microscope system may comprise a plurality of microscope modules, a cassette for holding a plurality of slides, a slide loader configured to move the plurality of slides between the cassette and the plurality of microscope modules, and a processor coupled to the slide loader. The processor may be configured with instructions which, when executed, cause the slide loader to move a slide into or from a selected microscope module among the plurality of microscope modules. Various other methods, systems, and computer-readable media are also disclosed.

公开(公告)号：US11863867B2

公开(公告)日：2024-01-02

申请号：US17309716

申请日：2020-08-05

Applicant: Leica Biosystems Imaging, Inc.

Inventor： Allen H. Olson ,  Yunlu Zou ,  Bharat Annaldas ,  Leng-Chun Chen

IPC: G02B21/00 ,  H04N23/67 ,  G02B21/24 ,  G02B21/36 ,  H04N3/06 ,  H04N23/90 ,  H04N23/56

CPC classification number: H04N23/67 ,  G02B21/002 ,  G02B21/244 ,  G02B21/367 ,  H04N3/06 ,  H04N23/90 ,  H04N23/56

Abstract: Real-time focusing in a slide-scanning system. In an embodiment, focus points are added to an initialized focus map while acquiring a plurality of image stripes of a sample on a glass slide. For each image stripe, a plurality of frames, collectively representing the image stripe, may be acquired using both an imaging line-scan camera and a tilted focusing line-scan camera. Focus points, representing positions of best focus for trusted frames, are added to the focus map. Outlying focus points are removed from the focus map. In some cases, one or more image stripes may be reacquired. Finally, the image stripes are assembled into a composite image of the sample.

公开(公告)号：US11860101B2

公开(公告)日：2024-01-02

申请号：US17583658

申请日：2022-01-25

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hongki Yoo ,  Wooseop Kim ,  Juehyung Kang ,  Ungyo Kang

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/04 ,  G02B21/16

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G02B21/002 ,  G02B21/04 ,  G02B21/16

Abstract: The multi-photon microscope comprises a repetition rate tuner that lowers an optical pulse train emitted from a pulsed laser to a repetition rate for time-gated detection, a scanner that scans the optical pulse train transmitted from the repetition rate tuner in x-axis and y-axis directions, an objective lens that irradiates an optical signal scanned by the scanner to the sample and acquires a fluorescence signal emitted from the excited fluorescent material, a photodetector that photoelectrically converts the fluorescence signal acquired by the objective lens, an amplifier that converts a current signal output from the photodetector into a voltage signal, a digitizer that samples the voltage signal output from the amplifier, and a computing device that separates sampling data output from the digitizer with a detection window set in time domain, and generates an image based on the sampling data separated by the detection window.

公开(公告)号：US20190227290A1

公开(公告)日：2019-07-25

申请号：US16182422

申请日：2018-11-06

Applicant: Carl Zeiss Microscopy GmbH ,  Carl Zeiss AG

Inventor： Daniel SCHWEDT ,  Matthias WALD ,  Tiemo ANHUT

IPC: G02B21/00

CPC classification number: G02B21/0032 ,  G02B21/002 ,  G02B21/004 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/008

Abstract: A functionally integrated laser scanning microscope for scanning a sample with laser illumination, selectably in a confocal, line or wide-field operating mode, comprising a laser light source, an illumination and detection beam path, a detection device and at least one objective, wherein the illumination and detection beam path has optical means for the configuration of the laser illumination, at least one scanner for scanning the sample with the laser illumination, and a beam splitter for separating illumination and detection light, and controllable optical elements for changing the beam guiding depending on the operating mode selected in each case.

公开(公告)号：US20190204574A1

公开(公告)日：2019-07-04

申请号：US16093542

申请日：2017-04-12

Applicant: CHARITÉ - UNIVERSITÄTSMEDIZIN BERLIN

Inventor： Benjamin JUDKEWITZ ,  Ioannis PAPADOPOULOS

IPC: G02B21/00

CPC classification number: G02B21/0072 ,  A61B5/0059 ,  G02B21/002 ,  G02B21/0056 ,  G02B21/0076 ,  G02B26/06

Abstract: It is provided a method for identifying optical aberrations. The method comprising the steps of providing at least one first optical beam and a second optical beam ; creating a combined beam by at least partially superimposing the first and the second optical beam; focusing the combined beam into or through a medium and detecting radiation excited in the medium by the combined beam due to nonlinear optical effects; detecting the radiation excited in the medium by the combined beam for each one of the phase positions, the spatial positions and/or the time positions of the first beam; and identifying aberrations using signals generated by a detection device for the plurality of the phase positions, the spatial positions and/or the time positions of the first beam relative to the second beam upon the detection of the radiation excited in the medium.

公开(公告)号：US20180329189A1

公开(公告)日：2018-11-15

申请号：US15615679

申请日：2017-06-06

Applicant: APPLIED MATERIALS, INC.

Inventor： Samer Banna ,  Waheb Bishara ,  Dong Wu ,  Mehdi Vaez-Iravani

IPC: G02B21/00 ,  G02B23/04

CPC classification number: G02B21/0032 ,  G02B21/002 ,  G02B21/0092 ,  G02B21/125

Abstract: A method for flexible inspection of a sample includes forming an input beam using a beam source, blocking a portion of the input beam using an input mask, and forming a shaped beam from a portion of the input beam. The shaped beam is received at a first portion of an objective lens and focused onto a sample. A reflected beam is collected at a second portion of the objective lens. Scattered light is collected at the first and second portions of the objective lens and at a third portion of the objective lens. The scattered light is received at a dark-field detector module and a portion of the scattered light is directed to a dark-field detector. The dark-field detector module includes an output mask having one or more output apertures that allow at least part of the scattered light that passes through the third portion of the object lens to pass as the portion of the scattered light that is directed to the dark-field detector.

公开(公告)号：US10073045B2

公开(公告)日：2018-09-11

申请号：US14416723

申请日：2013-07-24

Applicant: NOVA MEASURING INSTRUMENTS LTD.

Inventor： Gilad Barak ,  Yanir Hainick ,  Dror Shafir

IPC: G01N21/894 ,  G01N21/95 ,  G02B21/00 ,  G02B27/46 ,  G01N21/88

CPC classification number: G01N21/9501 ,  G01N21/8806 ,  G01N2021/8848 ,  G01N2201/061 ,  G01N2201/0683 ,  G01N2201/12 ,  G02B21/0016 ,  G02B21/002 ,  G02B21/0092 ,  G02B27/46

Abstract: An optical method and system are presented for use in measurement of isolated features of a structure. According to this technique, Back Focal Plane Microscopy (BFM) measurements are applied to a structure and measured data indicative thereof is obtained, wherein the BFM measurements utilize dark-field detection mode while applying pinhole masking to incident light propagating through an illumination channel towards the structure, the measured data being thereby indicative of a scattering matrix characterizing scattering properties of the structure, enabling identification of one or more isolated features of the structure.

公开(公告)号：US20180196246A1

公开(公告)日：2018-07-12

申请号：US15788778

申请日：2017-10-19

Applicant: Cornell University

Inventor： Amanda J. Bares ,  Chris B. Schaffer ,  Steven Tilley

IPC: G02B21/00 ,  G02B27/14 ,  G02B26/00 ,  G01N21/64

CPC classification number: G02B21/0064 ,  G01J3/28 ,  G01J3/4406 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6465 ,  G02B21/002 ,  G02B21/0032 ,  G02B21/0052 ,  G02B21/0076 ,  G02B21/16 ,  G02B26/007 ,  G02B26/105 ,  G02B27/141 ,  G02B2207/114

Abstract: Optical sensing techniques and devices based on detection of fluorescent emissions at different optical wavelengths by nonlinear optical absorption of different excitation beams at different excitation wavelengths that interact with fluorescently-labeled structures within the sample to cause nonlinear optical absorption of two or more photons at each excitation wavelength. The fluorescent light at different fluorescent emission wavelengths by nonlinear optical absorption of excitation light at a particular excitation wavelength is spectrally separated into different optical channel output beams along different optical channel optical paths at different designated fluorescent imaging wavelength bands and the fluorescent light at different fluorescent imaging wavelengths within each designated fluorescent imaging wavelength is detected. This two-stage spectral separation in obtaining fluorescent images at different fluorescent imaging wavelengths in different fluorescent imaging wavelength bands enables highly sensitive hyperspectral imaging based on two-photo or multi-photon nonlinear absorption.

公开(公告)号：US20180188517A1

公开(公告)日：2018-07-05

申请号：US15858487

申请日：2017-12-29

Applicant: Leica Biosystems Imaging, Inc.

Inventor： Yunlu ZOU ,  Nicholas NEWBERG ,  Gregory CRANDALL

IPC: G02B21/36 ,  G02B21/34 ,  G02B21/00 ,  G02B21/26

CPC classification number: G02B21/361 ,  G02B21/002 ,  G02B21/245 ,  G02B21/26 ,  G02B21/34 ,  G02B21/365 ,  G06T2207/10056

Abstract: A digital pathology imaging apparatus includes a single line scan camera sensor optically coupled with first and second optical paths. In a first embodiment, transmission mode illumination and oblique mode illumination are simultaneously used during a single stage movement that captures a low resolution macro image of the entire sample area and the entire label area of the slide via the first optical path. In a second embodiment, transmission mode illumination is used during a first stage movement that captures a low resolution macro image of at least the entire sample area via the first optical path and oblique mode illumination is used during a second stage movement that captures a low resolution macro image of at least the entire label area via the first optical path.