公开(公告)号：US20170145406A1

公开(公告)日：2017-05-25

申请号：US15425539

申请日：2017-02-06

Applicant: DNAE Group Holdings Limited

Inventor： Victor C. Esch ,  Sergey A. Dryga ,  Lisa-Jo Ann Clarizia

IPC: C12N15/10 ,  G01N33/569 ,  G01N33/543 ,  C12Q1/68

CPC classification number: G01N33/54326 ,  A61B5/055 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/043 ,  C07K1/22 ,  C07K16/1267 ,  C12N13/00 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/54333 ,  G01N33/56911 ,  G01N2333/195 ,  G01N2446/20 ,  G01R33/465 ,  Y10T428/2982 ,  C12Q2565/629 ,  C12Q2563/143

Abstract: The invention generally relates to conducting an assay on a sample that isolates a bacterium from the sample, in which the assay isolates as low as about 1 CFU/ml of bacteria in the sample.

公开(公告)号：US09562896B2

公开(公告)日：2017-02-07

申请号：US13091534

申请日：2011-04-21

Applicant: Victor C. Esch ,  Sergey A. Dryga ,  Lisa-Jo Ann Clarizia

Inventor： Victor C. Esch ,  Sergey A. Dryga ,  Lisa-Jo Ann Clarizia

IPC: G01N33/00 ,  G01N33/543 ,  G01N33/569 ,  C07K1/22 ,  C07K16/12 ,  C12N13/00 ,  C12Q1/68 ,  G01N24/08 ,  G01R33/465 ,  A61B5/055

CPC classification number: G01N33/54326 ,  A61B5/055 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/043 ,  C07K1/22 ,  C07K16/1267 ,  C12N13/00 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/54333 ,  G01N33/56911 ,  G01N2333/195 ,  G01N2446/20 ,  G01R33/465 ,  Y10T428/2982 ,  C12Q2565/629 ,  C12Q2563/143

Abstract: The invention generally relates to conducting an assay on a sample that isolates a bacterium from the sample, in which the assay isolates as low as about 1 CFU/ml of bacteria in the sample.

Abstract translation: 本发明一般涉及对从样品中分离出细菌的样品进行测定，其中测定分离出样品中低至约1CFU / ml的细菌。

公开(公告)号：US09488648B2

公开(公告)日：2016-11-08

申请号：US13852613

申请日：2013-03-28

Applicant: T2 Biosystems, Inc.

Inventor： Lori Anne Neely ,  Mark John Audeh ,  Matthew Blanco ,  James Franklin Chepin ,  Vasiliki Demas ,  Rahul K. Dhanda ,  Thomas Jay Lowery, Jr.

IPC: G01N33/543 ,  G01N24/08 ,  G01N27/74 ,  G01N33/94 ,  B82Y25/00 ,  C12Q1/68 ,  G01R33/09 ,  G01R33/12 ,  G01R33/28 ,  G01R33/30 ,  G01R33/44 ,  C12Q1/70

CPC classification number: G01N33/54326 ,  B82Y25/00 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q1/70 ,  C12Q1/701 ,  G01N24/08 ,  G01N27/745 ,  G01N33/5434 ,  G01N33/9493 ,  G01R33/093 ,  G01R33/1269 ,  G01R33/281 ,  G01R33/302 ,  G01R33/448 ,  C12Q2563/143 ,  C12Q2565/633 ,  C12Q2563/149 ,  C12Q2565/113

Abstract: This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.

公开(公告)号：US09389225B2

公开(公告)日：2016-07-12

申请号：US12855147

申请日：2010-08-12

Applicant: Sergey A. Dryga ,  Victor C. Esch ,  Richard G. Saul ,  Andrew F. McDowell

Inventor： Sergey A. Dryga ,  Victor C. Esch ,  Richard G. Saul ,  Andrew F. McDowell

IPC: G01N33/543 ,  G01N33/569 ,  C12Q1/68 ,  A61B5/055 ,  G01R33/465 ,  G01N24/08 ,  C07K1/22 ,  C07K16/12 ,  C12N13/00

CPC classification number: G01N33/54326 ,  A61B5/055 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/043 ,  C07K1/22 ,  C07K16/1267 ,  C12N13/00 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/54333 ,  G01N33/56911 ,  G01N2333/195 ,  G01N2446/20 ,  G01R33/465 ,  Y10T428/2982 ,  C12Q2565/629 ,  C12Q2563/143

Abstract: The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.

Abstract translation: 本发明一般涉及使用磁性颗粒和交替磁场将目标分析物与样品分离。 在某些实施方案中，本发明的方法包括将样品与包括对于目标分析物特异性的第一部分的磁性颗粒接触，从而在样品中形成靶/颗粒复合物，使样品流过包含连接至至少一个表面的第二部分的通道 通道，向流动的样品施加交变磁场以导致靶/颗粒复合物接近表面以结合样品中残留的第二部分和未结合的颗粒，将靶/颗粒复合物结合到第二部分， 并清洗样品中未结合的颗粒和未结合的分析物。

公开(公告)号：US20160033474A1

公开(公告)日：2016-02-04

申请号：US14774655

申请日：2014-03-13

Applicant: NESTEC S.A.

Inventor： Francois-Pierre Martin ,  Sebastiano Collino ,  Ivan Montoliu Roura

IPC: G01N33/493 ,  A61K31/715 ,  A61K31/733

CPC classification number: G01N33/493 ,  A61K31/715 ,  A61K31/733 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/50 ,  G01N33/6893 ,  G01N2570/00 ,  G01N2800/044 ,  G01N2800/50 ,  G01N2800/52 ,  G01N2800/70

Abstract: The present invention relates generally to the field of nutrition and health, particular, the present invention relates to alpha-keto-isovalerate as a biomarker urine of the efficacy of prebiotics for the prevention of diet induced weight gain.

Abstract translation: 本发明一般涉及营养和健康领域，特别地，本发明涉及α-酮 - 异戊酸酯作为益生元的预防饮食诱导体重增加功效的生物标志物尿。

公开(公告)号：US20150309051A1

公开(公告)日：2015-10-29

申请号：US14649072

申请日：2013-11-25

Applicant: NESTEC S.A.

Inventor： Francois-Pierre Martin ,  Claire L. Boulange ,  Ivan Montoliu Rora ,  Sebastiano Collino ,  Marc-Emmanuel Dumas ,  Elaine Holmes ,  Serge Andre Dominique Rezzi ,  Jeremy Nicholson ,  Sunil Kochhar

IPC: G01N33/68

CPC classification number: G01N33/6893 ,  C12Q1/61 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/50 ,  G01N33/6806 ,  G01N2800/044 ,  G01N2800/50 ,  G01N2800/60 ,  G01N2800/70

Abstract: The present invention relates generally to the field of nutrition and health. In particular, the present invention relates to a new biomarker, its use and a method that allows it to diagnose the likelihood to resist diet induced weight gain, and/or to be susceptible to a diet induced weight gain. For example, the biomarker may be isovalerylglycine.

Abstract translation: 本发明一般涉及营养和健康领域。 特别地，本发明涉及新的生物标志物，其用途和方法，其允许其诊断抵抗饮食诱导的体重增加的可能性，和/或易于饮食诱导的体重增加。 例如，生物标志物可以是异戊酰甘氨酸。

公开(公告)号：US07125665B2

公开(公告)日：2006-10-24

申请号：US10182559

申请日：2001-01-26

Applicant: Albert M. Bobst ,  Jeffery D. Hester

Inventor： Albert M. Bobst ,  Jeffery D. Hester

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q2565/633 ,  C12Q2561/101

Abstract: The present invention relates to methods and kits for detecting the presence or absence of a target DNA sequence, such as a mutation, within an identified region of a selected DNA molecule, such as a gene. In particular aspects, the invention relates to the use of certain aspects of the polymerase chain reaction (“PCR”) and ligase chain reaction (“LCR”) techniques for the detection of genetic mutations in genes, particularly point mutations. A kit has been developed for direct EPR detection of specific PCR amplified target nucleic acid sequences. The PCR reaction is carried out in the presence of nitroxide-labeled oligomers that are degraded only if hybridized to a complementary target sequence. The degradation of the nitroxide-labeled oligomers into nitroxide-labeled cleavage products results in a characteristic increase of the h-/ho ratio of the EPR signal; in the absence of a complementary target sequence the EPR signal of nitroxide-labeled oligomer remains unchanged.

公开(公告)号：US07038036B2

公开(公告)日：2006-05-02

申请号：US10214503

申请日：2002-08-07

Applicant: Gota Kawai ,  Akira Wada ,  Hiroshi Takaku

Inventor： Gota Kawai ,  Akira Wada ,  Hiroshi Takaku

IPC: C07H21/04

CPC classification number: C12N15/113 ,  C12N2310/3125 ,  C12N2310/315 ,  C12N2310/3181 ,  C12N2310/3517 ,  C12Q1/68 ,  Y10T436/24 ,  C12Q2565/633 ,  C12Q2563/101

Abstract: Antisense oligonucleotide sequences which enable the measurement of the distribution and structure of antisense oligonucleotide drugs in the body, with lapse of time, and a method of detecting these sequences are provided. The antisense chains have a natural or non-natural nucleotide or peptide nucleic acid as a structural unit in which carbon atoms and nitrogen atoms are substituted by 13C and 15N, respectively, and the antisense chains can be detected by nuclear magnetic resonance spectroscopy (NMR) such as 15N—1H or 13C—1H hetero nuclear multiple quantum coherence spectroscopy.

Abstract translation: 提供能够测量体内反义寡核苷酸药物在时间上的分布和结构的反义寡核苷酸序列，以及检测这些序列的方法。 反义链具有天然或非天然核苷酸或肽核酸作为结构单元，其中碳原子和氮原子分别被13个和15个N取代 ，并且可以通过核磁共振光谱（NMR）检测反义链，例如<！ - SIPO - > <！ - SIPO < 1异质核多重量子相干光谱。

公开(公告)号：US09869671B2

公开(公告)日：2018-01-16

申请号：US15496790

申请日：2017-04-25

Applicant: DNAE Group Holdings Limited

Inventor： Sergey A. Dryga ,  Lisa-Jo Ann Clarizia ,  Eddie W. Adams ,  Victor C. Esch

IPC: C12Q1/70 ,  G01N33/543 ,  C12Q1/68 ,  G01N33/569 ,  B01L3/00 ,  C07K1/22

CPC classification number: G01N33/54326 ,  A61B5/055 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/043 ,  C07K1/22 ,  C07K16/1267 ,  C12N13/00 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/54333 ,  G01N33/56911 ,  G01N2333/195 ,  G01N2446/20 ,  G01R33/465 ,  Y10T428/2982 ,  C12Q2565/629 ,  C12Q2563/143

Abstract: The invention generally relates to conducting an assay on a sample that isolates a pathogen from the sample and allows for analysis of the pathogen with minimal (i.e., at most 24 hrs of culturing) or no culturing of the pathogen. In certain embodiments, the invention provides methods for identifying a pathogen from a sample that involve obtaining a sample including a pathogen, conducting an assay that isolates the pathogen from the sample, culturing the isolated pathogen for at most about 24 hrs, and analyzing the pathogen.

公开(公告)号：US20140106442A1

公开(公告)日：2014-04-17

申请号：US14056796

申请日：2013-10-17

Applicant: T2 BIOSYSTEMS, INC.

Inventor： THOMAS JAY LOWERY, JR. ,  MARK JOHN AUDEH ,  MATTHEW BLANCO ,  JAMES FRANKLIN CHEPIN ,  VASILIKI DEMAS ,  RAHUL K. DHANDA ,  MARILYN LEE FRITZEMEIER ,  ISAAC KOH ,  SONIA KUMAR ,  LORI ANNE NEELY ,  BRIAN M. MOZELESKI ,  DANIELLA LYNN PLOURDE ,  CHARLES WILLIAM RITTERSHAUS ,  PARRIS S. WELLMAN

IPC: G01N27/74

CPC classification number: G01N27/745 ,  B82Y25/00 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q1/701 ,  C12Q2600/156 ,  G01N24/08 ,  G01N33/54326 ,  G01N33/5434 ,  G01R33/093 ,  G01R33/1269 ,  G01R33/281 ,  G01R33/30 ,  G01R33/302 ,  G01R33/448 ,  Y10T436/24 ,  C12Q2563/143 ,  C12Q2565/633

Abstract: This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease.

Abstract translation: 本发明的特征在于用于检测分析物的系统和方法及其在治疗和诊断疾病中的用途。