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公开(公告)号：US20180251814A1
公开(公告)日：2018-09-06
申请号：US15559689
申请日：2016-03-18
申请人： VILNIUS UNIVERSITY
IPC分类号： C12Q1/6806 , C12Q1/6853 , C12Q1/6876
CPC分类号： C12Q1/6806 , C12Q1/6853 , C12Q1/6876 , C12Q2521/125 , C12Q2563/137
摘要： Provided is a method for modifying a ssRNA at the 3′ end, said method comprising contacting the strand with a ssRNA 2′-O-methyltransferase in the presence of a co-factor, under conditions which allow for the transfer by the ssRNA 2′-O-methyltransferase of a part of the co-factor onto 3′ end of the ssRNA to form a modified ssRNA, wherein the ssRNA bears 2′-OH group at 3′ terminal nucleotide and wherein the part of the co-factor transferred comprises a reporter group or a functional group.
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公开(公告)号：US09988673B2
公开(公告)日：2018-06-05
申请号：US15072677
申请日：2016-03-17
申请人： Vilnius University
CPC分类号： C12Q1/6853 , C12P19/34 , C12Q1/6846 , C12Q1/6869 , C12Q1/6876 , C12Q2521/125 , C12Q2525/117 , C12Q2525/155 , C12Q2537/1373
摘要： A method for producing a nucleic acid molecule from a template nucleic acid sequence and a linking unit attached to a primer, which method comprises a step of contacting the template nucleic acid sequence with a nucleic acid polymerase under conditions which allow the nucleic acid polymerase to produce the nucleic acid molecule from the primer based on the template nucleic acid sequence, wherein the linking unit is attached to a target site in the template nucleic acid sequence with a covalent linkage.
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公开(公告)号：US20180073057A1
公开(公告)日：2018-03-15
申请号：US15817178
申请日：2017-11-18
申请人： Dimitra Tsavachidou
发明人： Dimitra Tsavachidou
CPC分类号： C12Q1/6806 , C12P19/34 , C12Q1/68 , C12Q1/6855 , C12Q1/6869 , C12Q2521/125 , C12Q2521/301 , C12Q2521/319 , C12Q2525/179 , C12Q2525/301
摘要： Methods for constructing consecutively connected and optionally truncated copies of nucleic acid molecules are disclosed. Consecutively connected copies of nucleic acid molecules can be used to perform sequencing of the same nucleic acid molecules several times, improving overall accuracy of sequencing. Sequencing of truncated copies of nucleic acid molecules can be used to deduce the sequences of nucleic acid molecules from assembling short sequenced segments. Connected copies of nucleic acid molecules can be constructed by first attaching hairpin adaptors to the nucleic acid molecules, and then using strand displacing polymerases to generate complementary strands of the nucleic acid molecule strands connected by the hairpin adaptors.
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公开(公告)号：US20150148240A1
公开(公告)日：2015-05-28
申请号：US14578125
申请日：2014-12-19
申请人： Timothy H. Bestor , John R. Edwards , Jingyue Ju , Xiaoxu Li
发明人： Timothy H. Bestor , John R. Edwards , Jingyue Ju , Xiaoxu Li
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C07H19/23 , C12Q1/6827 , C12Q1/6874 , C12Q2521/125 , C12Q2527/125 , C12Q2537/164
摘要： This invention provides methods of derivatizing a double-stranded DNA comprising contacting double-stranded DNA with a CpG methyltransferase and an s-adenosylmethionine analog. This invention also provides methods of sequencing DNA to determine methylation patterns.
摘要翻译： 本发明提供衍生双链DNA的方法，包括使双链DNA与CpG甲基转移酶和s-腺苷甲硫氨酸类似物接触。 本发明还提供了测序DNA以测定甲基化模式的方法。
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公开(公告)号：US20140363815A1
公开(公告)日：2014-12-11
申请号：US14363442
申请日：2012-12-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6846 , C12Q2523/125 , C12Q2531/113 , C12Q2521/125
摘要： The present invention relates to methods and kits for the detection of 5-hydroxymethylcytosine (5hmC) and/or 5-methylcytosine (5meC). In some embodiments, the present invention relates to methods and kits for detection of 5hmC and/or 5meC in nucleic acid (e.g., DNA, RNA). In some embodiments, the present invention relates to detection of 5hmC in genomic DNA, e.g., mammalian genomic DNA
摘要翻译： 本发明涉及用于检测5-羟甲基胞嘧啶（5hmC）和/或5-甲基胞嘧啶（5meC）的方法和试剂盒。 在一些实施方案中，本发明涉及用于检测核酸（例如DNA，RNA）中的5meC和/或5meC的方法和试剂盒。 在一些实施方案中，本发明涉及在基因组DNA例如哺乳动物基因组DNA中检测5hmC
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6.发明授权Derivatization of biomolecules by covalent coupling of non-cofactor compounds using methyltransferases 有权通过使用甲基转移酶的非辅因子化合物的共价偶联衍生生物分子公开(公告)号：US08822146B2
公开(公告)日：2014-09-02
申请号：US13262340
申请日：2010-04-01
CPC分类号： C12Q1/6827 , C07H21/00 , C12Q1/48 , C12Q1/6816 , G01N2333/91011 , C12Q2563/107 , C12Q2521/125
摘要： The present invention relates to a use of non-cofactor compounds, represented by formulas (I) or (II) wherein R and Z are independently selected from H, D, C1-C12-alkyl, preferably C1-C4-alkyl, alkenyl, alkinyl, phenyl or -LX, wherein X represents a functional group or a reporter group attached via a linker group L, and QH is selected from —SH, —SeH, —NHNH2 or —ONH2, for a targeted modification or derivatization of a biomolecule by covalent coupling to the biomolecule in the presence of a directing methyltransferase. Further development of the method of targeted modification and derivatization are the method for targeted labeling a biomolecule and method for detecting unmethylated target sites in a biomolecule comprising modification of the biomolecule according to the present invention.
摘要翻译： 本发明涉及由式（I）或（II）表示的非辅助因子化合物的用途，其中R和Z独立地选自H，D，C 1 -C 12烷基，优选C 1 -C 4烷基，烯基， 炔基，苯基或-LX，其中X表示通过连接基团L连接的官能团或报告基团，QH选自-SH，-SeH，-NHNH 2或-ONH 2，用于生物分子的靶向修饰或衍生化 通过在导向甲基转移酶的存在下与生物分子共价偶联。 靶向修饰和衍生化方法的进一步开发是用于靶向生物分子标记的方法和用于检测生物分子中未甲基化靶位点的方法，其包括根据本发明的生物分子的修饰。
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公开(公告)号：US20140213461A1
公开(公告)日：2014-07-31
申请号：US13965166
申请日：2013-08-12
发明人： Radoje Drmanac , Matthew J. Callow
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译： 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。
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公开(公告)号：US20120100534A1
公开(公告)日：2012-04-26
申请号：US12329365
申请日：2008-12-05
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6855 , C12Q2531/125 , C12Q2521/313 , C12Q2521/125 , C12Q2537/163 , C12Q2525/191 , C12Q2521/531 , C12Q2521/501 , C12Q2525/307 , C12Q2525/151
摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.
摘要翻译： 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。
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公开(公告)号：US20120070829A1
公开(公告)日：2012-03-22
申请号：US13229132
申请日：2011-09-09
申请人： Steven Okino , Yan Wang
发明人： Steven Okino , Yan Wang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6804 , C12Q1/6806 , C12Q1/6837 , C12Q2521/301 , C12Q2525/204 , C12Q2521/125 , C12Q2521/331
摘要： Methods for analyzing chromosomal DNA, including chromatin, are provided.
摘要翻译： 提供了分析染色体DNA（包括染色质）的方法。
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公开(公告)号：US20090305241A1
公开(公告)日：2009-12-10
申请号：US12006779
申请日：2008-01-04
申请人： Robert Fischer , Jon Penterman , Jin Hoe Huh , Tzung-Fu Hsieh
发明人： Robert Fischer , Jon Penterman , Jin Hoe Huh , Tzung-Fu Hsieh
CPC分类号： C12Q1/6844 , C12N9/0073 , C12N15/8216 , C12N15/8218 , C12N15/8261 , C12N15/827 , Y02A40/146 , C12Q2521/125
摘要： Polypeptides with DNA demethylase activity as well as methods of their use are provided.
摘要翻译： 提供了具有脱甲基酶活性的多肽及其使用方法。
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