METHODS FOR IMPROVING PLANT EMBRYO QUALITY AND GERMINATION

    公开(公告)号:US20170298321A1

    公开(公告)日:2017-10-19

    申请号:US15336303

    申请日:2016-10-27

    CPC classification number: C12N5/04 A01H4/001 A01H4/005 C12N2500/34 C12N2500/50

    Abstract: Provided is a method of producing plant embryos having improved embryo quality or germination frequency comparing to embryos developed by conventional methods. The method entails the steps of (a) incubating plant embryogenic suspensor mass (ESM) in, or on, a standard development medium supplied with glucose for a first incubation period to develop immature somatic embryos; (b) mass transferring the immature somatic embryos to a modified development medium near or at cotyledon stage; and (c) incubating the immature somatic embryos in, or on, the modified development medium for a second incubation period to develop mature somatic embryos. The modified development medium contains reduced concentration of glucose or is glucose-free and the osmolality of the modified development medium is adjusted to compensate for the loss of osmolality due to reduction or removal of glucose.

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