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公开(公告)号：US12010963B2
公开(公告)日：2024-06-18
申请号：US17720652
申请日：2022-04-14
发明人： Yangyan Zhou , Weijian Sun , Wenxiu Chen , Aowei Mo , Peng Guo , Penghao Xu
CPC分类号： A01H4/002 , A01G22/05 , A01G24/15 , A01G24/22 , A01H4/001 , A01H5/08 , A01H6/368 , C12N5/0025 , C12N5/04 , C12N2500/05 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/60 , C12N2501/30 , C12N2533/76
摘要： The invention provides a callus induction medium for blueberry tissue culture, taking woody plant medium (WPM) as a basic medium, and including: 0.5-5.0 mg/L forchlorfenuron (CPPU) and 0.1-0.4 mg/L 2-isopentenyladenine (2-ip). The present invention also provides a callus culture method for blueberry, including inoculating the blueberry explant into the above callus induction medium to conduct induction culture in order to form blueberry callus. The present invention also discloses the medium combination and culture method to culture the above blueberry callus to blueberry tissue culture plant. For the above medium and culture method, the differentiation effect is good, efficiency is high, one can conduct continuous differentiation, and the effect is better on multiple varieties.
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公开(公告)号：US11925166B2
公开(公告)日：2024-03-12
申请号：US17617215
申请日：2020-06-05
申请人： Mars, Incorporated
摘要： Provided herein are methods for establishing Theobroma cell suspension cultures using young leaves as a source of explants. Also provided are induction, proliferation, and suspension media used for producing Theobroma cell suspension cultures. These methods and media may be useful for producing secondary metabolites in Theobroma, as well as for isolating virus particles associated with Theobroma diseases.
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公开(公告)号：US20240260529A1
公开(公告)日：2024-08-08
申请号：US18439198
申请日：2024-02-12
申请人： Lowes TC Pty Ltd
发明人： Greg Lowe
CPC分类号： A01H4/001 , A01G2/10 , A01G9/0293 , A01G9/0299 , A01G27/005 , A01H4/002 , A01H4/003
摘要： A plant propagation system is provided that includes a holder for holding at least two plants in relative spaced apart relation to enable a predetermined operation (such as, for example, a cutting operation) to be performed on each of the plants within the holder during a single pass.
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公开(公告)号：US12022786B2
公开(公告)日：2024-07-02
申请号：US17431036
申请日：2020-02-14
申请人： Muhammed Majeed , Kalyanam Nagabhushanam , Natarajan Sankaran , Sivakumar Arumugam , Muthuraman Gnanamani , Kulithalai Viswanathan Krishnamurthy , Ugraiah Amilineni
发明人： Muhammed Majeed , Kalyanam Nagabhushanam , Natarajan Sankaran , Sivakumar Arumugam , Muthuraman Gnanamani , Kulithalai Viswanathan Krishnamurthy , Ugraiah Amilineni
摘要： The present invention discloses a method of isolating secondary metabolites, specifically arjunolic acid, from the calli and/or the suspension cultures derived from the pluripotent cambium tissue of Terminalia arjuna. The invention also discloses a method of inducing callus, establishing and maintaining suspension cultures of callus derived from the cambium of Terminalia arjuna for the isolation of secondary metabolites.
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公开(公告)号：US20240284845A1
公开(公告)日：2024-08-29
申请号：US18587413
申请日：2024-02-26
申请人： PROFILE PRODUCTS LLC
发明人： Michael Dan ROBESON , Daniel Scott NORDEN , Stanton Reid SMITH , Gary Lane BOWERS , Ryan Michael Knauer , Timothy Daniel Knauer
IPC分类号： A01H4/00
CPC分类号： A01H4/002
摘要： A fibrous propagation medium includes a volume of refined, acidic wood and/or bark fiber having about 2.3-5.0 vol. % of a particle size of 2.36-4.74 mm (sieve #8), based on the total volume of the wood fiber, moisture content of about 16-28 wt. %, and water holding capacity of about 50-95%, according to the NCSU Promoter Analysis.
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公开(公告)号：US20230180689A1
公开(公告)日：2023-06-15
申请号：US18079850
申请日：2022-12-12
发明人： Xiaolu Chen , Fulai Yu , Yongfeng Xiao , Yulan Li , Qin Luo , Ping Yu , Yinghua CHEN , Yong YANG , Kai WANG , Chao YUAN , Dan WANG , Lingliang GUAN , Yingbo ZHANG , Mei HUANG , Xiaoli XIE , Zhenxia CHEN
摘要： The present disclosure provides a method for obtaining adventitious tetraploid buds of Blumea balsamifera, comprising the following steps: selecting a root segment of diploid B. balsamifera as an explant, culturing the root segment in a chromosome doubling inducing medium supplemented with 0.025-0.1 mg/L 1-naphthaleneacetic acid (NAA), 1.0-2.0 mg/L 6-benzylaminopurine (6-BA), and 90-150 mg/L colchicine, inducing explant cells, and simultaneously doubling chromosomes and differentiating the adventitious buds. The present disclosure fills the blank of using a root of B. balsamifera as the explant and increases effective explant sources during the propagation, proliferation and biotechnological breeding of B. balsamifera. More importantly, root cells of the B. balsamifera are directly differentiated into adventitious buds while chromosomes are doubled, and a callus formation process is not needed, so that the technical links are simplified and the variation of regeneration buds and the generation of chimeras are reduced.
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公开(公告)号：US20240315187A1
公开(公告)日：2024-09-26
申请号：US18615464
申请日：2024-03-25
申请人： PROFILE PRODUCTS LLC
IPC分类号： A01H4/00
摘要： A propagation plug includes an irradiated, hydrophilic substrate body comprising a growing medium having bound organic fibers, the substrate body being rewettable and having a moisture content of less than about 20 wt. %, based on the total weight of the body, as measured according to ASTM test method no. D2216 and being sterile as evidenced by having an aerobic bacterial count of
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公开(公告)号：US20240008428A1
公开(公告)日：2024-01-11
申请号：US18038428
申请日：2021-11-24
发明人： Neal Wright
摘要： An apparatus (100) for use in culturing Sphagnum (106) is provided. The apparatus (100) comprises a culture vessel (102) for Sphagnum (106) and a culture medium (104) arranged in the culture vessel (102). The culture vessel (102) comprises an enclosed airspace (108) above the culture medium (104), and Sphagnum (106) arranged in the culture medium (104). The apparatus (100) further comprises means (110, 112) for supplying carbon dioxide into the enclosed airspace (108).
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公开(公告)号：US20230404005A1
公开(公告)日：2023-12-21
申请号：US18209503
申请日：2023-06-14
发明人： Kaile MAI , Rongzhen LI , Changsan ZHU , Lixin FENG , Yuanchang LU , Xianzhao LIU , Jienan SU , Jianhua LAN , Debao LIAO , Kun QIN
IPC分类号： A01H4/00
摘要： A tissue culture method and a propagation method of Cathaya argyrophylla are described herein. An adventitious bud can be induced from an explant of the Cathaya argyrophylla using a suitable induction medium, with an induction rate reaching 69.22% to 73.33%. The induced adventitious bud is inoculated into a suitable proliferation medium, such that the adventitious bud can proliferate and strengthen seedlings, where the adventitious bud shows a proliferation rate reaching 46.23% to 55%. The adventitious bud after proliferation and seedling strengthening are inoculated into a suitable rooting medium to allow rooting culture, thereby obtaining a complete tissue culture seedling of the Cathaya argyrophylla. The tissue culture method is of great significance for protecting the Cathaya argyrophylla, increasing a plant quantity of the Cathaya argyrophylla, and expanding a population of the Cathaya argyrophylla to prevent extinction.
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公开(公告)号：US11800842B2
公开(公告)日：2023-10-31
申请号：US18079850
申请日：2022-12-12
发明人： Xiaolu Chen , Fulai Yu , Yongfeng Xiao , Yulan Li , Qin Luo , Ping Yu , Yinghua Chen , Yong Yang , Kai Wang , Chao Yuan , Dan Wang , Lingliang Guan , Yingbo Zhang , Mei Huang , Xiaoli Xie , Zhenxia Chen
摘要： The present disclosure provides a method for obtaining adventitious tetraploid buds of Blumea balsamifera, comprising the following steps: selecting a root segment of diploid B. balsamifera as an explant, culturing the root segment in a chromosome doubling inducing medium supplemented with 0.025-0.1 mg/L 1-naphthaleneacetic acid (NAA), 1.0-2.0 mg/L 6-benzylaminopurine (6-BA), and 90-150 mg/L colchicine, inducing explant cells, and simultaneously doubling chromosomes and differentiating the adventitious buds. The present disclosure fills the blank of using a root of B. balsamifera as the explant and increases effective explant sources during the propagation, proliferation and biotechnological breeding of B. balsamifera. More importantly, root cells of the B. balsamifera are directly differentiated into adventitious buds while chromosomes are doubled, and a callus formation process is not needed, so that the technical links are simplified and the variation of regeneration buds and the generation of chimeras are reduced.
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