公开(公告)号：US20230404005A1

公开(公告)日：2023-12-21

申请号：US18209503

申请日：2023-06-14

申请人： GX ECO-ENGINEERING VOCATIONAL & TECHNICAL COLLEGE

发明人： Kaile MAI ,  Rongzhen LI ,  Changsan ZHU ,  Lixin FENG ,  Yuanchang LU ,  Xianzhao LIU ,  Jienan SU ,  Jianhua LAN ,  Debao LIAO ,  Kun QIN

IPC分类号： A01H4/00

CPC分类号： A01H4/008 ,  A01H4/002

摘要： A tissue culture method and a propagation method of Cathaya argyrophylla are described herein. An adventitious bud can be induced from an explant of the Cathaya argyrophylla using a suitable induction medium, with an induction rate reaching 69.22% to 73.33%. The induced adventitious bud is inoculated into a suitable proliferation medium, such that the adventitious bud can proliferate and strengthen seedlings, where the adventitious bud shows a proliferation rate reaching 46.23% to 55%. The adventitious bud after proliferation and seedling strengthening are inoculated into a suitable rooting medium to allow rooting culture, thereby obtaining a complete tissue culture seedling of the Cathaya argyrophylla. The tissue culture method is of great significance for protecting the Cathaya argyrophylla, increasing a plant quantity of the Cathaya argyrophylla, and expanding a population of the Cathaya argyrophylla to prevent extinction.