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公开(公告)号:US20220338435A1
公开(公告)日:2022-10-27
申请号:US17720652
申请日:2022-04-14
发明人: Yangyan Zhou , Weijian Sun , Wenxiu Chen , Aowei Mo , Peng Guo , Penghao Xu
摘要: The invention provides a callus induction medium for blueberry tissue culture, it takes WPM medium as basic medium, comprises: 0.5-5.0 mg/L CPPU and 0.1-0.4 mg/L 2-ip. The present invention also provides a callus culture method of blueberry, the step thereof comprises: inoculating the blueberry explant into the above callus induction medium to conduct induction culture, to form blueberry callus. The present invention also discloses the medium combination and culture method to culture the above blueberry callus to blueberry tissue culture plant. For the above medium and culture method, the differentiation effect is good, efficiency is high, and can conduct continuous differentiation, and the effect to multiple varieties are all better.
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公开(公告)号:US11864512B2
公开(公告)日:2024-01-09
申请号:US17720726
申请日:2022-04-14
发明人: Yangyan Zhou , Weijian Sun , Wenxiu Chen , Aowei Mo , Peng Guo , Penghao Xu
CPC分类号: A01H4/001 , A01G17/005 , A01G24/15 , A01G24/22 , A01H4/008
摘要: The invention discloses a medium combination for a rapid propagation medium for blueberry stem segment tissue, wherein the pre-culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising 0.1-0.5 mg/L IAA, 0.05-0.6 mg/L CPPU; the rapid culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.2-0.5 mg/L ZT; the seedling growth medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.1-2.0 mg/L 2-ip; and the rooting medium takes ½ MS medium as basis medium, comprising: 0.3-4.0 mg/L NAA. The invention further discloses a method of using the above medium combination to conduct rapid propagation of blueberry stem segment tissue, the propagation rate of this method is rapid.
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公开(公告)号:US20220330507A1
公开(公告)日:2022-10-20
申请号:US17720726
申请日:2022-04-14
发明人: Yangyan Zhou , Weijian Sun , Wenxiu Chen , Aowei Mo , Peng Guo , Penghao Xu
摘要: The invention discloses a medium combination for a rapid propagation medium for blueberry stein segment tissue, wherein the pre-culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising 0.1-0.5 mg/L IAA, 0.05-0.6 mg/L CPPU; the rapid culture medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.2-0.5 mg/L ZT; the seedling growth medium takes the MS medium containing 2-(N-morpholine) ethanesulfonic acid as basic medium, comprising: 0.1-2.0 mg/L 2-ip; and the rooting medium takes ½ MS medium as basis medium, comprising: 0.3-4.0 mg/L NAA. The invention further discloses a method of using the above medium combination to conduct rapid propagation of blueberry stein segment tissue, the propagation rate of this method is rapid.
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公开(公告)号:US12010963B2
公开(公告)日:2024-06-18
申请号:US17720652
申请日:2022-04-14
发明人: Yangyan Zhou , Weijian Sun , Wenxiu Chen , Aowei Mo , Peng Guo , Penghao Xu
CPC分类号: A01H4/002 , A01G22/05 , A01G24/15 , A01G24/22 , A01H4/001 , A01H5/08 , A01H6/368 , C12N5/0025 , C12N5/04 , C12N2500/05 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/60 , C12N2501/30 , C12N2533/76
摘要: The invention provides a callus induction medium for blueberry tissue culture, taking woody plant medium (WPM) as a basic medium, and including: 0.5-5.0 mg/L forchlorfenuron (CPPU) and 0.1-0.4 mg/L 2-isopentenyladenine (2-ip). The present invention also provides a callus culture method for blueberry, including inoculating the blueberry explant into the above callus induction medium to conduct induction culture in order to form blueberry callus. The present invention also discloses the medium combination and culture method to culture the above blueberry callus to blueberry tissue culture plant. For the above medium and culture method, the differentiation effect is good, efficiency is high, one can conduct continuous differentiation, and the effect is better on multiple varieties.
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