公开(公告)号：US12129495B2

公开(公告)日：2024-10-29

申请号：US18046895

申请日：2022-10-14

申请人： Codexis, Inc.

发明人： Ericka Bermudez ,  David Elgart ,  Nikki D. Kruse ,  Mathew G. Miller ,  Vesna Mitchell ,  Jovana Nazor ,  Nandhitha Subramanian

IPC分类号： C12N9/10 ,  C12N9/12 ,  C12N15/63

CPC分类号： C12N9/1252 ,  C12N15/63 ,  C12Y207/07007

摘要： The present disclosure relates to engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The present disclosure also provides methods of using the engineered DNA polymerase polypeptides or compositions thereof for diagnostic and other purposes.

公开(公告)号：US12129494B2

公开(公告)日：2024-10-29

申请号：US17558454

申请日：2021-12-21

申请人： Codexis, Inc.

发明人： Mathew G. Miller ,  Chinping Chng ,  Oscar Alvizo ,  Melissa Ann Mayo ,  James Nicholas Riggins ,  Xiang Yi ,  Jonathan S. Penfield ,  Gjalt W. Huisman ,  Jared Davis ,  Yasushi Saotome

IPC分类号： C12N9/12 ,  C12P19/34 ,  C12N15/00

CPC分类号： C12N9/1247 ,  C12P19/34 ,  C12Y207/07006 ,  C12N15/00

摘要： The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5′)ppp(5′)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

公开(公告)号：US20240318152A1

公开(公告)日：2024-09-26

申请号：US18516054

申请日：2023-11-21

申请人： Codexis, Inc.

发明人： David Entwistle ,  Jason Fell ,  Stephan Jenne ,  Anders Matthew Knight ,  Simon Ng ,  Ryan D. Reeves ,  Zara Maxine Seibel ,  Jonathan Vroom

IPC分类号： C12N9/02

CPC分类号： C12N9/0071 ,  C12Y114/14001

摘要： The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity on a 1-tert-Butoxycarbonylaminocyclopentanoic acid substrate.

公开(公告)号：US12071642B2

公开(公告)日：2024-08-27

申请号：US18462237

申请日：2023-09-06

申请人： Codexis, Inc. ,  Societe des Produits Nestle S.A.

发明人： Chinping Chng ,  Nikki Dellas ,  Da Duan ,  Ravi David Garcia ,  Harvinder Chagger Maniar

IPC分类号： C12N9/26 ,  A61K38/47 ,  A61P1/18 ,  C12N15/09 ,  C12N15/10 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12N9/2414 ,  A61K38/47 ,  A61P1/18 ,  C12Y302/01001

摘要： The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH

公开(公告)号：US12060592B2

公开(公告)日：2024-08-13

申请号：US17089498

申请日：2020-11-04

申请人： Codexis Inc.

发明人： Steven J. Collier ,  Ee Ling Teo ,  Joly Sukumaran ,  Robert J. Wilson ,  Junye Xu

IPC分类号： C12N9/10 ,  C07K14/00 ,  C12N15/52 ,  C12P7/62 ,  C12P17/06

CPC分类号： C12P17/06 ,  C07K14/00 ,  C12N9/1025 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/62 ,  C12Y203/01 ,  Y02P20/52

摘要： Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.

公开(公告)号：US20240263154A1

公开(公告)日：2024-08-08

申请号：US18628461

申请日：2024-04-05

申请人： Codexis, Inc.

发明人： Mathew G. Miller ,  Vesna Mitchell ,  Jovana Nazor ,  Donald S. Baskerville ,  Nikki Dellas ,  David Elgart ,  Jonathan Vroom ,  Sandy M. Gomes ,  Nandhitha Subramanian ,  Ericka Bermudez

IPC分类号： C12N9/12

CPC分类号： C12N9/1252

摘要： The present invention provides engineered DNA polymerase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered DNA polymerase polypeptides. The invention also provides methods for use of the compositions comprising the engineered DNA polymerase polypeptides for diagnostic and other purposes.

公开(公告)号：US20240228982A9

公开(公告)日：2024-07-11

申请号：US18471524

申请日：2023-09-21

申请人： Codexis, Inc.

发明人： Oscar Alvizo ,  Melissa Ann Mayo ,  Jeffrey C. Moore

IPC分类号： C12N9/06 ,  C12P13/00

CPC分类号： C12N9/0028 ,  C12P13/001 ,  C07K14/195

摘要： The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US20240209408A1

公开(公告)日：2024-06-27

申请号：US18485925

申请日：2023-10-12

申请人： Codexis, Inc.

发明人： David Entwistle ,  Stephanie Marie Forgel ,  Anders Matthew Knight ,  Mikayla Krawczyk ,  Philip Provencher ,  Amani Shoubber ,  Jonathan Vroom ,  David Watts ,  Leland Wong

IPC分类号： C12P19/38 ,  C12N9/02 ,  C12N9/12

CPC分类号： C12P19/38 ,  C12N9/0008 ,  C12N9/1205 ,  C12Y102/03003 ,  C12Y207/01

摘要： The present invention provides engineered 3′O-kinase polypeptides useful for construction of materials used in template-independent polynucleotide synthesis, as well as compositions and methods of utilizing these engineered polypeptides. The present disclosure also describes one-pot methods for conversion of a natural or modified nucleoside to a nucleoside tetraphosphate or NQP.

公开(公告)号：US11987818B2

公开(公告)日：2024-05-21

申请号：US17330686

申请日：2021-05-26

申请人： Codexis, Inc.

发明人： Margie Tabuga Borra-Garske ,  Oscar Alvizo ,  Melissa Ann Mayo ,  Stephan Jenne ,  Auric Anthony Sowell-Kantz ,  Carmela Molinaro

IPC分类号： C12N9/06 ,  C12N9/02

CPC分类号： C12N9/0028 ,  C12N9/0036 ,  C12Y105/01 ,  C12Y106/00

摘要： The present application provides engineered polypeptides having imine or oxime reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US20240141304A1

公开(公告)日：2024-05-02

申请号：US18472540

申请日：2023-09-22

申请人： Codexis, Inc.

发明人： Onorato Campopiano ,  Emily Mundorff ,  Birthe Borup ,  Rama Voladri

IPC分类号： C12N9/04 ,  C12P17/10

CPC分类号： C12N9/0006 ,  C12P17/10 ,  C12Y101/01184 ,  Y02P20/52

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.