公开(公告)号：US20240209408A1

公开(公告)日：2024-06-27

申请号：US18485925

申请日：2023-10-12

申请人： Codexis, Inc.

发明人： David Entwistle ,  Stephanie Marie Forgel ,  Anders Matthew Knight ,  Mikayla Krawczyk ,  Philip Provencher ,  Amani Shoubber ,  Jonathan Vroom ,  David Watts ,  Leland Wong

IPC分类号： C12P19/38 ,  C12N9/02 ,  C12N9/12

CPC分类号： C12P19/38 ,  C12N9/0008 ,  C12N9/1205 ,  C12Y102/03003 ,  C12Y207/01

摘要： The present invention provides engineered 3′O-kinase polypeptides useful for construction of materials used in template-independent polynucleotide synthesis, as well as compositions and methods of utilizing these engineered polypeptides. The present disclosure also describes one-pot methods for conversion of a natural or modified nucleoside to a nucleoside tetraphosphate or NQP.