公开(公告)号：US20240182876A1

公开(公告)日：2024-06-06

申请号：US18485855

申请日：2023-10-12

申请人： Codexis, Inc.

发明人： David Entwistle ,  Stephanie Forget ,  Michelle Li ,  Niusha Mahmoodi ,  Ryan Reeves ,  Ljubica Vojcic ,  Jonathan Vroom ,  David Watts ,  Leland Ken Wong

IPC分类号： C12N9/12 ,  C12N15/63

CPC分类号： C12N9/1264 ,  C12N15/63 ,  C12Y207/07031

摘要： The present invention provides engineered terminal deoxynucleotidyl transferase (TdT) polypeptides useful in template-independent polynucleotide synthesis, as well as compositions, methods of utilizing these engineered polypeptides, and polynucleotides encoding the engineered terminal deoxynucleotidyl transferases.

公开(公告)号：US20240010999A1

公开(公告)日：2024-01-11

申请号：US18364419

申请日：2023-08-02

申请人： Codexis, Inc.

发明人： Jonathan Vroom ,  Stephanie Sue Galanie ,  Jack Liang ,  Joyce Liu ,  Nikki Dellas ,  Melissa Ann Mayo ,  David Entwistle

IPC分类号： C12N9/10 ,  C07H15/252 ,  C12P19/56

CPC分类号： C12N9/1062 ,  C12N9/1048 ,  C07H15/252 ,  C12P19/56 ,  C12Y204/01013

摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

公开(公告)号：US20180223264A1

公开(公告)日：2018-08-09

申请号：US15886094

申请日：2018-02-01

申请人： Codexis, Inc.

发明人： Jonathan Vroom ,  Stephanie Sue Galanie ,  Nikki Dellas ,  Jack Liang ,  Joyce Liu ,  David Entwistle ,  Courtney Dianne Moffett

IPC分类号： C12N9/10 ,  C12N9/88 ,  C12P19/56 ,  C12P19/18

CPC分类号： C12N9/1048 ,  C12N9/1051 ,  C12N9/88 ,  C12P19/18 ,  C12P19/56 ,  C12Y204/01 ,  C12Y204/01013

摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

公开(公告)号：US20220106575A1

公开(公告)日：2022-04-07

申请号：US17553455

申请日：2021-12-16

申请人： Codexis, Inc.

发明人： Oscar Alvizo ,  Nicholas J. Agard ,  Xinkai Xie ,  David Entwistle ,  Birgit Kosjek

IPC分类号： C12N9/04 ,  C12P17/12 ,  C12P41/00 ,  C12P7/40 ,  C12P7/02

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US20220056431A1

公开(公告)日：2022-02-24

申请号：US17522370

申请日：2021-11-09

申请人： Codexis, Inc.

发明人： Scott J. Novick ,  Ravi David Garcia ,  Charlene Ching ,  Nikki Dellas ,  David Entwistle ,  Vesna Mitchell

IPC分类号： C12N9/88

摘要： The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.

公开(公告)号：US11236308B2

公开(公告)日：2022-02-01

申请号：US16874574

申请日：2020-05-14

申请人： Codexis, Inc.

发明人： Oscar Alvizo ,  Nicholas J. Agard ,  Xinkai Xie ,  David Entwistle ,  Birgit Kosjek

IPC分类号： C12N9/04 ,  C12P41/00 ,  C12P7/02 ,  C12P17/12 ,  C12P7/40

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

公开(公告)号：US11198861B2

公开(公告)日：2021-12-14

申请号：US16441458

申请日：2019-06-14

申请人： Codexis, Inc.

发明人： Scott J. Novick ,  Ravi David Garcia ,  Charlene Ching ,  Nikki Dellas ,  David Entwistle ,  Vesna Mitchell

IPC分类号： C12N9/88 ,  C12N9/06 ,  C12N1/20 ,  C12N15/00 ,  C12N15/70

摘要： The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.

公开(公告)号：US20210054348A1

公开(公告)日：2021-02-25

申请号：US17081904

申请日：2020-10-27

申请人： Codexis, Inc.

发明人： Jonathan Vroom ,  Stephanie Sue Galanie ,  Nikki Dellas ,  Jack Liang ,  Joyce Liu ,  David Entwistle ,  Courtney Dianne Moffett

IPC分类号： C12N9/10 ,  C12P19/18 ,  C12P19/56 ,  C12N9/88

摘要： The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

公开(公告)号：US20210032605A1

公开(公告)日：2021-02-04

申请号：US17072910

申请日：2020-10-16

申请人： Codexis, Inc.

发明人： Jovana Nazor ,  Robert Osborne ,  Jack Liang ,  Jonathan Vroom ,  Xiyun Zhang ,  David Entwistle ,  Rama Voladri ,  Ravi David Garcia ,  Jeffrey C. Moore ,  Shane Grosser ,  Birgit Kosjek ,  Matthew Truppo

IPC分类号： C12N9/02 ,  C12P17/12

摘要： The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

公开(公告)号：US20190177707A1

公开(公告)日：2019-06-13

申请号：US16218003

申请日：2018-12-12

申请人： Codexis, Inc.

发明人： Rama Voladri ,  David Entwistle ,  Christopher Michael Micklitsch ,  Vesna Mitchell ,  Nikki Dellas ,  Xiyun Zhang ,  Antoinette Sero ,  Brent Dorr ,  Douglas E. Fuerst

IPC分类号： C12N9/18

摘要： The present invention provides engineered carboxyesterase enzymes having improved properties as compared to a naturally occurring wild-type carboxyesterase enzymes, as well as polynucleotides encoding the engineered carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes in amidation reactions.